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The map label for this gene is mepA [H]

Identifier: 110677762

GI number: 110677762

Start: 357522

End: 358451

Strand: Direct

Name: mepA [H]

Synonym: RD1_0362

Alternate gene names: 110677762

Gene position: 357522-358451 (Clockwise)

Preceding gene: 110677761

Following gene: 110677763

Centisome position: 8.65

GC content: 61.18

Gene sequence:

>930_bases
ATGAAAACGTCACATTTCATCCTGATATCCGTGCTTGCAGTCGGCCTGATGGCCTGTTCCGAACAAAGCGCGCCACCTGC
GCAGGTCGCGCCATTGCCGACGATCGGCACCTCCGGGCATAAAGACAGCCTGAAACCGGCAAAACAGCTTTTCGGGGCGA
AATCAGCAGCCTCGCAAAGCCCGCCCGCTCCCTTCGGCAGCTATGCGAAAGGGTGCGTCGCAGGGGCCGCACAACTGCCT
GAAACAGGCCCCACATGGCAGGCGATGCGGCTGTCGCGCAACCGCAACTGGGCACACCCCGAAACGGTGAGCTTTGTGAA
AGACCTCAGCCGGATCGCAGCGCAGCAACCGGGATGGAATGGTCTCTATGTCGGGGATTTGAGCCAGCCGCGCGGGGGTC
CGATGCTGTCGGGTCATGCCAGTCACCAGATCGGGCTGGACGCAGACATCTGGCTGCGCCCTGCCACAAACCTGTCGCTG
AGCGTGGCGGAACGCGAAAACATCTCCTCCATTTCACTGCGGCGCAATGCCGGGGCCTTTGTCAACGACAACTGGACCCC
TCAACATGCCGCGATTCTCAAGGCCGCCGCATCGGACCCGCGCGTCGCGCGCATCTTCATCTTTCCCGGCGCAAAGGTTC
AGCTTTGCAACACAGCGACAGGTGACCGGTCATGGTTGCGCAAGATCAGACCGTGGTTCGGGCATCACTATCATTTCCAT
GTGCGCCTGTCCTGTCCGAGCGGATCGACCGGCTGTGTGGATCAAACCCCGCCACCCGCAGGGGACGGATGCGCGGATGC
GCAGGCGTGGGTCAATAATATCCTGAACCCGCCCCCACCTGATCCCAATGCGAAACCTCGAAAGCCAAGGCCGCCCGTGC
GCCTTGCTGATCTGCCTGCACAATGCGCCGACGTTTTACAATCGCAATAA

Upstream 100 bases:

>100_bases
ACCTCACCGGCAGCCAGCGTATGGGGATCACACCGATTGTTGTGCTTTTCATTCTGGGCCTGATCCTGCTCATATGGGTC
AAACCCAAAGGGGACTACGC

Downstream 100 bases:

>100_bases
TCAGCGCCGTGGCCCTCGCGCCGTGGTTCTGGTTCAAGCCTGTCGCGCCGCCGGATCAACCCGCTCACCTGTTGGCAGTA
ACCGACATCCCCCGCGACGA

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKTSHFILISVLAVGLMACSEQSAPPAQVAPLPTIGTSGHKDSLKPAKQLFGAKSAASQSPPAPFGSYAKGCVAGAAQLP
ETGPTWQAMRLSRNRNWAHPETVSFVKDLSRIAAQQPGWNGLYVGDLSQPRGGPMLSGHASHQIGLDADIWLRPATNLSL
SVAERENISSISLRRNAGAFVNDNWTPQHAAILKAAASDPRVARIFIFPGAKVQLCNTATGDRSWLRKIRPWFGHHYHFH
VRLSCPSGSTGCVDQTPPPAGDGCADAQAWVNNILNPPPPDPNAKPRKPRPPVRLADLPAQCADVLQSQ

Sequences:

>Translated_309_residues
MKTSHFILISVLAVGLMACSEQSAPPAQVAPLPTIGTSGHKDSLKPAKQLFGAKSAASQSPPAPFGSYAKGCVAGAAQLP
ETGPTWQAMRLSRNRNWAHPETVSFVKDLSRIAAQQPGWNGLYVGDLSQPRGGPMLSGHASHQIGLDADIWLRPATNLSL
SVAERENISSISLRRNAGAFVNDNWTPQHAAILKAAASDPRVARIFIFPGAKVQLCNTATGDRSWLRKIRPWFGHHYHFH
VRLSCPSGSTGCVDQTPPPAGDGCADAQAWVNNILNPPPPDPNAKPRKPRPPVRLADLPAQCADVLQSQ
>Mature_309_residues
MKTSHFILISVLAVGLMACSEQSAPPAQVAPLPTIGTSGHKDSLKPAKQLFGAKSAASQSPPAPFGSYAKGCVAGAAQLP
ETGPTWQAMRLSRNRNWAHPETVSFVKDLSRIAAQQPGWNGLYVGDLSQPRGGPMLSGHASHQIGLDADIWLRPATNLSL
SVAERENISSISLRRNAGAFVNDNWTPQHAAILKAAASDPRVARIFIFPGAKVQLCNTATGDRSWLRKIRPWFGHHYHFH
VRLSCPSGSTGCVDQTPPPAGDGCADAQAWVNNILNPPPPDPNAKPRKPRPPVRLADLPAQCADVLQSQ

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=247, Percent_Identity=40.080971659919, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33019; Mature: 33019

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSHFILISVLAVGLMACSEQSAPPAQVAPLPTIGTSGHKDSLKPAKQLFGAKSAASQS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCC
PPAPFGSYAKGCVAGAAQLPETGPTWQAMRLSRNRNWAHPETVSFVKDLSRIAAQQPGWN
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GLYVGDLSQPRGGPMLSGHASHQIGLDADIWLRPATNLSLSVAERENISSISLRRNAGAF
EEEECCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCCCCE
VNDNWTPQHAAILKAAASDPRVARIFIFPGAKVQLCNTATGDRSWLRKIRPWFGHHYHFH
ECCCCCCHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEE
VRLSCPSGSTGCVDQTPPPAGDGCADAQAWVNNILNPPPPDPNAKPRKPRPPVRLADLPA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCH
QCADVLQSQ
HHHHHHHCC
>Mature Secondary Structure
MKTSHFILISVLAVGLMACSEQSAPPAQVAPLPTIGTSGHKDSLKPAKQLFGAKSAASQS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCC
PPAPFGSYAKGCVAGAAQLPETGPTWQAMRLSRNRNWAHPETVSFVKDLSRIAAQQPGWN
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GLYVGDLSQPRGGPMLSGHASHQIGLDADIWLRPATNLSLSVAERENISSISLRRNAGAF
EEEECCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCCCCE
VNDNWTPQHAAILKAAASDPRVARIFIFPGAKVQLCNTATGDRSWLRKIRPWFGHHYHFH
ECCCCCCHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEE
VRLSCPSGSTGCVDQTPPPAGDGCADAQAWVNNILNPPPPDPNAKPRKPRPPVRLADLPA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCH
QCADVLQSQ
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]