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The map label for this gene is potB [H]

Identifier: 110677587

GI number: 110677587

Start: 174850

End: 176490

Strand: Direct

Name: potB [H]

Synonym: RD1_0179

Alternate gene names: 110677587

Gene position: 174850-176490 (Clockwise)

Preceding gene: 110677586

Following gene: 110677588

Centisome position: 4.23

GC content: 58.87

Gene sequence:

>1641_bases
ATGACCGATACCGCTGAACACTCCGGCCCGATGCTTGCCGCGGACGGCCGTCCGCTCAAGGCAAGCCTGAACCGTGCGTT
GCGCCGCCAAAAGATGCGCGCGCTCTTGCTGATTGCGCCGCTGTTGCTGTTCGTGCTGGTCACGTTCATCGCGCCGATTG
CGGACATGCTGTTCCGCTCTGTCGAAAACCAGATTGTATCCGACACATTGCCCCGCACCACGACCGAGCTGGCGACATGG
GATGCGGATAGCGGTGAAATCCCGTCGCAGGAAACGTTCAACGCGCTGTTCAAGGACGTCTTTATCGCGGCGGAGCGTAA
AGCGCATACGCGGCTGGGGTCCCGGCTCAACTATGAATTGACGGGTGTGTCATCCCTGTTTCGCAAATCAGGCCGCAGCG
TCGATGACATGGCGGAGGTCTATCAGGACCAGTTCGAAGACCTCAACGACTTCTGGAAAGAGGGCGAAAACTGGAGCCAG
TTGATGGGCTCGGCTTCGTGGCTCACCGCGATGAACGGCTGGAATGAGGATAGCGGACAGGCGCAGCCGGTCTTCGAGGT
GCGCGACGACATCGCCGCCGCCTTGCCGGAAACGACTGCCATCTATGAGGAATTCGCAACATTCGTTCAGATCGAGGAAG
AAGAACGCCTCACCACCGAAGACCCCTGGGCGGTTGTTTATGCGGCGCTCTATGAGGATATGGCAGGCGGCGCGGACCTG
TCGGGCATCTCCGGTGCGGGCAGTGCTGAGCTTCTGGCCGCCGCTGAAGCCGCAGCGACGTTTGAGACGGTCGATTATAC
CGCCGCCTTTTATGAGGTTGACGAGGATTGGCACGACATCGACGTCTGGCAGACCATCCAGACTTATACCCCCGACTTCA
CCTCCGGCTATTTCCTCAACGCGGTTGATATGAAGAAATCGCCGGACGGCCCGGAAATGCGCCCGGAAAACGAACAGCTG
CACATCATGTTGTTCATGCGCACATTGTTCATGTCTCTGGTGATCACCGGAAGCTGCATTCTGCTCGGCTATCCCGTGGC
GTGGATCCTGGCCAACCTGCCCGCGCGCACGGCGAACCTGTTGATGATCCTCGTGTTGTTGCCCTTCTGGACATCGCTCT
TGGTGCGCACATCCGCATGGAAGGTCATGTTGCAGCAACAGGGCGTCGTGAATGATACCCTTGTCTGGCTTGGGATTGTG
GCCGATGACAGTCGCCTTGCGCTGATAAACAACCAGACCGGCACGATCATCGCGATGACGCATATCCTGCTGCCGTTCAT
GATCCTGCCGCTCTATTCGGTGATGAAGACGATCCCGCCGAGTTATCTGCGCGCCGCCAAGTCATTGGGGGCCACGAACT
GGACAGCCTTCTGGCGGGTCTATTTCCCGCAATCCGTGCCGGGGATCGGCGCGGGGTCGATCCTCGTGTTCATCCTGTCC
ATCGGCTATTACATCACGCCTGAAATCGTCGGGGGCACCACGGGTACGTTCATTTCCAACCGGATCGCCTATCACATTTC
CAGCTCGCTCAACTGGGGCCTTGCCGCCGCACTCGGGGCCATCCTGCTGGCGGTCGTTCTGGCGCTCTACTGGGCATATG
ATAGAATTGTTGGCATCGACAACGTGAAATTGGGAGGCTGA

Upstream 100 bases:

>100_bases
CGCGCTCGCGTTCTACAATGACCCCGACTATCAGGAAAACATGAAAATCCGACAGGCAAACGCCGACGGATCCATACTCG
TTGTGGAGGGCGTTTGAGCC

Downstream 100 bases:

>100_bases
CACATGGTAGCTCTGACACCTGTATCCCGGACGGAGCCGTCCTTTTATGTCCCTGTTGTCGCTGCGTTCGGCGCCTTTGC
AGGCCTTTTTGTCGGCACAG

Product: ABC transporter, permease protein, putative

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTDTAEHSGPMLAADGRPLKASLNRALRRQKMRALLLIAPLLLFVLVTFIAPIADMLFRSVENQIVSDTLPRTTTELATW
DADSGEIPSQETFNALFKDVFIAAERKAHTRLGSRLNYELTGVSSLFRKSGRSVDDMAEVYQDQFEDLNDFWKEGENWSQ
LMGSASWLTAMNGWNEDSGQAQPVFEVRDDIAAALPETTAIYEEFATFVQIEEEERLTTEDPWAVVYAALYEDMAGGADL
SGISGAGSAELLAAAEAAATFETVDYTAAFYEVDEDWHDIDVWQTIQTYTPDFTSGYFLNAVDMKKSPDGPEMRPENEQL
HIMLFMRTLFMSLVITGSCILLGYPVAWILANLPARTANLLMILVLLPFWTSLLVRTSAWKVMLQQQGVVNDTLVWLGIV
ADDSRLALINNQTGTIIAMTHILLPFMILPLYSVMKTIPPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILS
IGYYITPEIVGGTTGTFISNRIAYHISSSLNWGLAAALGAILLAVVLALYWAYDRIVGIDNVKLGG

Sequences:

>Translated_546_residues
MTDTAEHSGPMLAADGRPLKASLNRALRRQKMRALLLIAPLLLFVLVTFIAPIADMLFRSVENQIVSDTLPRTTTELATW
DADSGEIPSQETFNALFKDVFIAAERKAHTRLGSRLNYELTGVSSLFRKSGRSVDDMAEVYQDQFEDLNDFWKEGENWSQ
LMGSASWLTAMNGWNEDSGQAQPVFEVRDDIAAALPETTAIYEEFATFVQIEEEERLTTEDPWAVVYAALYEDMAGGADL
SGISGAGSAELLAAAEAAATFETVDYTAAFYEVDEDWHDIDVWQTIQTYTPDFTSGYFLNAVDMKKSPDGPEMRPENEQL
HIMLFMRTLFMSLVITGSCILLGYPVAWILANLPARTANLLMILVLLPFWTSLLVRTSAWKVMLQQQGVVNDTLVWLGIV
ADDSRLALINNQTGTIIAMTHILLPFMILPLYSVMKTIPPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILS
IGYYITPEIVGGTTGTFISNRIAYHISSSLNWGLAAALGAILLAVVLALYWAYDRIVGIDNVKLGG
>Mature_545_residues
TDTAEHSGPMLAADGRPLKASLNRALRRQKMRALLLIAPLLLFVLVTFIAPIADMLFRSVENQIVSDTLPRTTTELATWD
ADSGEIPSQETFNALFKDVFIAAERKAHTRLGSRLNYELTGVSSLFRKSGRSVDDMAEVYQDQFEDLNDFWKEGENWSQL
MGSASWLTAMNGWNEDSGQAQPVFEVRDDIAAALPETTAIYEEFATFVQIEEEERLTTEDPWAVVYAALYEDMAGGADLS
GISGAGSAELLAAAEAAATFETVDYTAAFYEVDEDWHDIDVWQTIQTYTPDFTSGYFLNAVDMKKSPDGPEMRPENEQLH
IMLFMRTLFMSLVITGSCILLGYPVAWILANLPARTANLLMILVLLPFWTSLLVRTSAWKVMLQQQGVVNDTLVWLGIVA
DDSRLALINNQTGTIIAMTHILLPFMILPLYSVMKTIPPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILSI
GYYITPEIVGGTTGTFISNRIAYHISSSLNWGLAAALGAILLAVVLALYWAYDRIVGIDNVKLGG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=223, Percent_Identity=34.0807174887892, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=33.6363636363636, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787713, Length=225, Percent_Identity=32.8888888888889, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 60359; Mature: 60228

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTAEHSGPMLAADGRPLKASLNRALRRQKMRALLLIAPLLLFVLVTFIAPIADMLFRS
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VENQIVSDTLPRTTTELATWDADSGEIPSQETFNALFKDVFIAAERKAHTRLGSRLNYEL
HHHHHHHHCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
TGVSSLFRKSGRSVDDMAEVYQDQFEDLNDFWKEGENWSQLMGSASWLTAMNGWNEDSGQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCCCCC
AQPVFEVRDDIAAALPETTAIYEEFATFVQIEEEERLTTEDPWAVVYAALYEDMAGGADL
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHEECHHCCCCCCCCHHHHHHHHHHHHCCCCCC
SGISGAGSAELLAAAEAAATFETVDYTAAFYEVDEDWHDIDVWQTIQTYTPDFTSGYFLN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEE
AVDMKKSPDGPEMRPENEQLHIMLFMRTLFMSLVITGSCILLGYPVAWILANLPARTANL
EEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
LMILVLLPFWTSLLVRTSAWKVMLQQQGVVNDTLVWLGIVADDSRLALINNQTGTIIAMT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHH
HILLPFMILPLYSVMKTIPPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
IGYYITPEIVGGTTGTFISNRIAYHISSSLNWGLAAALGAILLAVVLALYWAYDRIVGID
HCHHCCCHHCCCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVKLGG
CEECCC
>Mature Secondary Structure 
TDTAEHSGPMLAADGRPLKASLNRALRRQKMRALLLIAPLLLFVLVTFIAPIADMLFRS
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VENQIVSDTLPRTTTELATWDADSGEIPSQETFNALFKDVFIAAERKAHTRLGSRLNYEL
HHHHHHHHCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
TGVSSLFRKSGRSVDDMAEVYQDQFEDLNDFWKEGENWSQLMGSASWLTAMNGWNEDSGQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCCCCC
AQPVFEVRDDIAAALPETTAIYEEFATFVQIEEEERLTTEDPWAVVYAALYEDMAGGADL
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHEECHHCCCCCCCCHHHHHHHHHHHHCCCCCC
SGISGAGSAELLAAAEAAATFETVDYTAAFYEVDEDWHDIDVWQTIQTYTPDFTSGYFLN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEE
AVDMKKSPDGPEMRPENEQLHIMLFMRTLFMSLVITGSCILLGYPVAWILANLPARTANL
EEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
LMILVLLPFWTSLLVRTSAWKVMLQQQGVVNDTLVWLGIVADDSRLALINNQTGTIIAMT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHH
HILLPFMILPLYSVMKTIPPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
IGYYITPEIVGGTTGTFISNRIAYHISSSLNWGLAAALGAILLAVVLALYWAYDRIVGID
HCHHCCCHHCCCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVKLGG
CEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]