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The map label for this gene is mcbR [H]

Identifier: 110677582

GI number: 110677582

Start: 169773

End: 170432

Strand: Direct

Name: mcbR [H]

Synonym: RD1_0171

Alternate gene names: 110677582

Gene position: 169773-170432 (Clockwise)

Preceding gene: 110677576

Following gene: 110677583

Centisome position: 4.11

GC content: 54.39

Gene sequence:

>660_bases
ATGGAATACGTAAAACTCCCGGCGCATCAATCCGTTTATGAGGATATCCGCGACCAGATCCTTTTTGGTGACATGGCGCC
GGGGCAGCCGGTGACCATCCAGGGGCTGGCTGATACACTCAAGGCGGGTATGACACCAGTGCGTGAGGCCATACGCAGGC
TGACTTCTGACGGGGCGTTGAACATGACTGGCAATCGACGTGTGAGCGTGCCGGTTATCACGCACACATGCCTTGAAGAG
CTTGAATTCATGCGAAAAACGCTGGAACCAGAACTGGCTTTCCGCGCTGTTTCGAAAATTACGGATGCTGCGAAAGAGAT
GCTTCATGCCCATGACGACGCGCTGAATTCAGCCATCTCGCAGGGCGATATCAAAGGGTATCTGACGCATAATTACCACT
TTCATGCGATCCTGTACCAAGCGGCACAGGCCCCCATCATGACCGCCACAGTGGATCGACTGTGGTTGCGGTTCGGACCT
TCCTTACGGGTTGTGTGTGGTCGGCATGGCACCATGAACCTGCCTGATAAACACGCAGATCTGCTTGCCGCATTGGACAG
CAAAGACGCCAGATGCGTCCGTGAAGCGATGGCTGAGGACGTCCATCAGGGCATCATTCAAATCCGGGAAGCTCTTGATG
CGACAATGAAACCGGATTGA

Upstream 100 bases:

>100_bases
TCATGCGAGATGAGCTTGTGCAAGCGATGCATGTTTTGCACAAACAAAGGAAGACACAGTTGCGCTGGCGCTGCTTGAAA
AAAAGGCAAAAGGACGTCTT

Downstream 100 bases:

>100_bases
TTCGATTGACTCCTCGTAATTTGATCATATTCTGAAATCGAAACCCTATTGTACATTTCAACAAAGGCAGGTGCCCTATG
TCCGCTATCTCGAATCATAT

Product: transcriptional regulator, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MEYVKLPAHQSVYEDIRDQILFGDMAPGQPVTIQGLADTLKAGMTPVREAIRRLTSDGALNMTGNRRVSVPVITHTCLEE
LEFMRKTLEPELAFRAVSKITDAAKEMLHAHDDALNSAISQGDIKGYLTHNYHFHAILYQAAQAPIMTATVDRLWLRFGP
SLRVVCGRHGTMNLPDKHADLLAALDSKDARCVREAMAEDVHQGIIQIREALDATMKPD

Sequences:

>Translated_219_residues
MEYVKLPAHQSVYEDIRDQILFGDMAPGQPVTIQGLADTLKAGMTPVREAIRRLTSDGALNMTGNRRVSVPVITHTCLEE
LEFMRKTLEPELAFRAVSKITDAAKEMLHAHDDALNSAISQGDIKGYLTHNYHFHAILYQAAQAPIMTATVDRLWLRFGP
SLRVVCGRHGTMNLPDKHADLLAALDSKDARCVREAMAEDVHQGIIQIREALDATMKPD
>Mature_219_residues
MEYVKLPAHQSVYEDIRDQILFGDMAPGQPVTIQGLADTLKAGMTPVREAIRRLTSDGALNMTGNRRVSVPVITHTCLEE
LEFMRKTLEPELAFRAVSKITDAAKEMLHAHDDALNSAISQGDIKGYLTHNYHFHAILYQAAQAPIMTATVDRLWLRFGP
SLRVVCGRHGTMNLPDKHADLLAALDSKDARCVREAMAEDVHQGIIQIREALDATMKPD

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=198, Percent_Identity=29.7979797979798, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787821, Length=219, Percent_Identity=25.1141552511416, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24340; Mature: 24340

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYVKLPAHQSVYEDIRDQILFGDMAPGQPVTIQGLADTLKAGMTPVREAIRRLTSDGAL
CCCEECCCHHHHHHHHHHHEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHCCCCE
NMTGNRRVSVPVITHTCLEELEFMRKTLEPELAFRAVSKITDAAKEMLHAHDDALNSAIS
EECCCCEEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDIKGYLTHNYHFHAILYQAAQAPIMTATVDRLWLRFGPSLRVVCGRHGTMNLPDKHAD
CCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
LLAALDSKDARCVREAMAEDVHQGIIQIREALDATMKPD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEYVKLPAHQSVYEDIRDQILFGDMAPGQPVTIQGLADTLKAGMTPVREAIRRLTSDGAL
CCCEECCCHHHHHHHHHHHEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHCCCCE
NMTGNRRVSVPVITHTCLEELEFMRKTLEPELAFRAVSKITDAAKEMLHAHDDALNSAIS
EECCCCEEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDIKGYLTHNYHFHAILYQAAQAPIMTATVDRLWLRFGPSLRVVCGRHGTMNLPDKHAD
CCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
LLAALDSKDARCVREAMAEDVHQGIIQIREALDATMKPD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]