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The map label for this gene is ptsN [H]

Identifier: 110635990

GI number: 110635990

Start: 3955191

End: 3955655

Strand: Reverse

Name: ptsN [H]

Synonym: Meso_3665

Alternate gene names: 110635990

Gene position: 3955655-3955191 (Counterclockwise)

Preceding gene: 110635991

Following gene: 110635987

Centisome position: 89.65

GC content: 60.43

Gene sequence:

>465_bases
ATGGATCTGAGCGATCTTATCGAGCCTTCGGCGATCCTGCCTGCCTTGAAGGCGAATTCCAAAAAGCAGGTGTTGCAGCT
GCTGGCGGAGAAAGCCGCTGAGCGAACCGGTCTGCCGGAACGCGAAATCTTCGAAACCATCCTGCAACGGGAAAAACTTG
GCTCCACCGGGGTCGGCAACGGTATTGCCATCCCGCATGGGAAGCTTCCCGGCATCAAGCAAATCACCGGCGTTTTCGCC
AGGCTGGAAACGCCGGTGGAATTCGATTCGCTCGACGATCTTCCCGTCGATCTGGTGTTCCTGCTGCTTGCGCCGGAGGG
CGCAGGAGCCGATCATTTGAAGGCACTTTCGCGCATTGCCCGTGTGCTGCGCGACGCGGATACGACCGCTAAGATACGCG
GCACGCGCGACGCCGCCGCCATCCACACCTTCTTGTCGGAAGCTCCCGCCTCGAACGCTGCCTGA

Upstream 100 bases:

>100_bases
GCGTGCCGATGGCAATATCGGATGGATCGATCCTTCCGCTACAAACGGATAGGCTGGGCCGCCGGCCCGTCAAAGGGAAT
GGCAAGCAGGGGAATATGAA

Downstream 100 bases:

>100_bases
ATTCGGAGTCCTTTCGCCACGCAAACCGCTTGCGTGGCGCGTCATCCCATAGTTTGCCGTCTAGTGAAGACTGACGGGCT
TGAGATCGTTCTGGAAAGCA

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MDLSDLIEPSAILPALKANSKKQVLQLLAEKAAERTGLPEREIFETILQREKLGSTGVGNGIAIPHGKLPGIKQITGVFA
RLETPVEFDSLDDLPVDLVFLLLAPEGAGADHLKALSRIARVLRDADTTAKIRGTRDAAAIHTFLSEAPASNAA

Sequences:

>Translated_154_residues
MDLSDLIEPSAILPALKANSKKQVLQLLAEKAAERTGLPEREIFETILQREKLGSTGVGNGIAIPHGKLPGIKQITGVFA
RLETPVEFDSLDDLPVDLVFLLLAPEGAGADHLKALSRIARVLRDADTTAKIRGTRDAAAIHTFLSEAPASNAA
>Mature_154_residues
MDLSDLIEPSAILPALKANSKKQVLQLLAEKAAERTGLPEREIFETILQREKLGSTGVGNGIAIPHGKLPGIKQITGVFA
RLETPVEFDSLDDLPVDLVFLLLAPEGAGADHLKALSRIARVLRDADTTAKIRGTRDAAAIHTFLSEAPASNAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=39.8648648648649, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI1786951, Length=136, Percent_Identity=30.8823529411765, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1788726, Length=129, Percent_Identity=34.1085271317829, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16382; Mature: 16382

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSDLIEPSAILPALKANSKKQVLQLLAEKAAERTGLPEREIFETILQREKLGSTGVGN
CCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLPGIKQITGVFARLETPVEFDSLDDLPVDLVFLLLAPEGAGADHLKALSRIA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RVLRDADTTAKIRGTRDAAAIHTFLSEAPASNAA
HHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDLSDLIEPSAILPALKANSKKQVLQLLAEKAAERTGLPEREIFETILQREKLGSTGVGN
CCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLPGIKQITGVFARLETPVEFDSLDDLPVDLVFLLLAPEGAGADHLKALSRIA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RVLRDADTTAKIRGTRDAAAIHTFLSEAPASNAA
HHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]