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The map label for this gene is tilS [H]

Identifier: 110635497

GI number: 110635497

Start: 3440968

End: 3442308

Strand: Reverse

Name: tilS [H]

Synonym: Meso_3168

Alternate gene names: 110635497

Gene position: 3442308-3440968 (Counterclockwise)

Preceding gene: 110635498

Following gene: 110635496

Centisome position: 78.01

GC content: 66.74

Gene sequence:

>1341_bases
TTGCTGAGCGACACGGCCTCGCCGGAACCGGGCGCGCTCTTTTCTGACATCCGCTTCGCCGGACGCAAAACGGTTGTTGC
GGCGGTTTCGGGCGGCGGCGATTCACTCGCCCTTCTCTTTCTCCTTCAAGATTTTCTGGAACGGCAGCCTTCGGGACCAT
CGCTCCTGGCGGTGACCGTCGATCATGGGCTCAGGCCCGACTCGGCAACGGAAGCGCGAGCTGTCGGCGAACTCGCCGCT
GCGCGGGGCATCGCTCACCGCACGCTTAGATGGGAAGAAGCAAAGCCCAAAGCCGGCATTTCCGCCGCGGCGCGGGAGGC
GCGATTGGCCCTGCTTGCGCGCGCTGCGCGCGAAGCGGGAACGGATCTCGTTTTCACGGCACACACCGCCGACGACCAGG
CGGAAACGCTGGCTATGCGGCTTTCGCGGGGCGGCGGGCGCGGCGCGGCGGGGATTGCGCCCGCCACGCTCTTTGAAGGG
GACGTCTGGTTCACGCGGCCGCTGCTTCACACGCGTCGCGCGGCGCTCAGGACATATCTCGGTGAGCGCGGCATCAGCTG
GTTCGAGGACCCGAGCAACCGGGATCCGCGTTTCGAGCGGGCTCGGGTGCGCGCCTGCCTGGACGAAGACCGTACCCAGG
CCCTTCTGGCGGAGGCGGAAAATGCGCAGCGCCTTCGGCAGGAGCAAGGGCGGGCCGCCGCAGATCTCATCGGGAGCGCC
GCGACGCGTGTCGCTCCCGGCCTCCTTCGTTTCGCGCTCGCGGCCGCTTCGGAAGAAAAAGGCGGGCTCTATGCGCTTCG
CATCGTGCTTGCCGTGGCCGGCGGCAGCCCTCATCTGGCCGACGAAGCGCGCTCGGCGGCGCTCCTGCGCCGCGCCCTTG
AGGATGAGCGGTGCCGCGTCAGTCTTTCCCGCGCCGTCGTTACGAGAAAGCGGGATTGGCTGTATCTCCATCGCGAAAGG
CGCAACCTGCCGGAGACTTCCCTTTCCGGCGAGCAGATCTGGGACGGGCGCTACCGTCTGGTTCCGGCAGGCCAATTGAA
AGGCGCGTGCGTTGCTGCTTTCGGCCCGGCAAACGCGGCTGCCCGCGATGTTGCGGAAGCGGAAGCGCCAGCCGGCCTTC
TACGGTCGGCACTTGCGGCTGAGCCGGCCATCTGGCGGGGTGATCTTTGCCTTGGGCTTGCAAGCGATATGGAGGGGCTT
GTGCTCCAGCGCCTGGCCCAACCCTGGGCAAGGCTTCTGCCCTCTTTCGACCTTGCTCCCGCGTCCGCTCTTCTGGAACT
CTTGAACGGTTCTGTGCCGCCGATCCTGCCATTCGATGGCCATAAAGAACAGATGGGATAA

Upstream 100 bases:

>100_bases
GCGACGTGGCATGCGCGACCTATACCGAGATCGGCCATCGTTACCCGGACGTCTCCTCCGCGCTCAAGGAGCGCGTGAAG
CAGGAACAGGCGCTTGCGGG

Downstream 100 bases:

>100_bases
ACCATGCTGCTCGGATTTGCCTTTCATATTGCCGGAATGCTTGGCAACGCTGCGCTTGCTCCCTATGTTAGGGAAGACGA
TGCCGGCCTCGGCGCACTCA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLSDTASPEPGALFSDIRFAGRKTVVAAVSGGGDSLALLFLLQDFLERQPSGPSLLAVTVDHGLRPDSATEARAVGELAA
ARGIAHRTLRWEEAKPKAGISAAAREARLALLARAAREAGTDLVFTAHTADDQAETLAMRLSRGGGRGAAGIAPATLFEG
DVWFTRPLLHTRRAALRTYLGERGISWFEDPSNRDPRFERARVRACLDEDRTQALLAEAENAQRLRQEQGRAAADLIGSA
ATRVAPGLLRFALAAASEEKGGLYALRIVLAVAGGSPHLADEARSAALLRRALEDERCRVSLSRAVVTRKRDWLYLHRER
RNLPETSLSGEQIWDGRYRLVPAGQLKGACVAAFGPANAAARDVAEAEAPAGLLRSALAAEPAIWRGDLCLGLASDMEGL
VLQRLAQPWARLLPSFDLAPASALLELLNGSVPPILPFDGHKEQMG

Sequences:

>Translated_446_residues
MLSDTASPEPGALFSDIRFAGRKTVVAAVSGGGDSLALLFLLQDFLERQPSGPSLLAVTVDHGLRPDSATEARAVGELAA
ARGIAHRTLRWEEAKPKAGISAAAREARLALLARAAREAGTDLVFTAHTADDQAETLAMRLSRGGGRGAAGIAPATLFEG
DVWFTRPLLHTRRAALRTYLGERGISWFEDPSNRDPRFERARVRACLDEDRTQALLAEAENAQRLRQEQGRAAADLIGSA
ATRVAPGLLRFALAAASEEKGGLYALRIVLAVAGGSPHLADEARSAALLRRALEDERCRVSLSRAVVTRKRDWLYLHRER
RNLPETSLSGEQIWDGRYRLVPAGQLKGACVAAFGPANAAARDVAEAEAPAGLLRSALAAEPAIWRGDLCLGLASDMEGL
VLQRLAQPWARLLPSFDLAPASALLELLNGSVPPILPFDGHKEQMG
>Mature_446_residues
MLSDTASPEPGALFSDIRFAGRKTVVAAVSGGGDSLALLFLLQDFLERQPSGPSLLAVTVDHGLRPDSATEARAVGELAA
ARGIAHRTLRWEEAKPKAGISAAAREARLALLARAAREAGTDLVFTAHTADDQAETLAMRLSRGGGRGAAGIAPATLFEG
DVWFTRPLLHTRRAALRTYLGERGISWFEDPSNRDPRFERARVRACLDEDRTQALLAEAENAQRLRQEQGRAAADLIGSA
ATRVAPGLLRFALAAASEEKGGLYALRIVLAVAGGSPHLADEARSAALLRRALEDERCRVSLSRAVVTRKRDWLYLHRER
RNLPETSLSGEQIWDGRYRLVPAGQLKGACVAAFGPANAAARDVAEAEAPAGLLRSALAAEPAIWRGDLCLGLASDMEGL
VLQRLAQPWARLLPSFDLAPASALLELLNGSVPPILPFDGHKEQMG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=183, Percent_Identity=31.1475409836066, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 47734; Mature: 47734

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDTASPEPGALFSDIRFAGRKTVVAAVSGGGDSLALLFLLQDFLERQPSGPSLLAVTV
CCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
DHGLRPDSATEARAVGELAAARGIAHRTLRWEEAKPKAGISAAAREARLALLARAAREAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
TDLVFTAHTADDQAETLAMRLSRGGGRGAAGIAPATLFEGDVWFTRPLLHTRRAALRTYL
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
GERGISWFEDPSNRDPRFERARVRACLDEDRTQALLAEAENAQRLRQEQGRAAADLIGSA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ATRVAPGLLRFALAAASEEKGGLYALRIVLAVAGGSPHLADEARSAALLRRALEDERCRV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SLSRAVVTRKRDWLYLHRERRNLPETSLSGEQIWDGRYRLVPAGQLKGACVAAFGPANAA
HHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCEECCCEEEEECCCCCCCEEEEECCCCHH
ARDVAEAEAPAGLLRSALAAEPAIWRGDLCLGLASDMEGLVLQRLAQPWARLLPSFDLAP
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ASALLELLNGSVPPILPFDGHKEQMG
HHHHHHHHCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MLSDTASPEPGALFSDIRFAGRKTVVAAVSGGGDSLALLFLLQDFLERQPSGPSLLAVTV
CCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
DHGLRPDSATEARAVGELAAARGIAHRTLRWEEAKPKAGISAAAREARLALLARAAREAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
TDLVFTAHTADDQAETLAMRLSRGGGRGAAGIAPATLFEGDVWFTRPLLHTRRAALRTYL
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
GERGISWFEDPSNRDPRFERARVRACLDEDRTQALLAEAENAQRLRQEQGRAAADLIGSA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ATRVAPGLLRFALAAASEEKGGLYALRIVLAVAGGSPHLADEARSAALLRRALEDERCRV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SLSRAVVTRKRDWLYLHRERRNLPETSLSGEQIWDGRYRLVPAGQLKGACVAAFGPANAA
HHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCEECCCEEEEECCCCCCCEEEEECCCCHH
ARDVAEAEAPAGLLRSALAAEPAIWRGDLCLGLASDMEGLVLQRLAQPWARLLPSFDLAP
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ASALLELLNGSVPPILPFDGHKEQMG
HHHHHHHHCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]