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The map label for this gene is purA

Identifier: 110635488

GI number: 110635488

Start: 3428101

End: 3429399

Strand: Reverse

Name: purA

Synonym: Meso_3159

Alternate gene names: 110635488

Gene position: 3429399-3428101 (Counterclockwise)

Preceding gene: 110635490

Following gene: 110635486

Centisome position: 77.72

GC content: 62.36

Gene sequence:

>1299_bases
ATGGCGAATGTGGTGGTCGTCGGCTCGCAGTGGGGCGACGAAGGCAAGGGAAAAATTGTCGATTGGCTTTCCGAGCGCGC
GGATGTGGTCGTGCGTTATCAGGGTGGCCACAACGCTGGCCATACGCTTGTGATCGATGGGGTGAGCTACAAGCTTTCGC
TGCTGCCCTCGGGAGTGGTGCGGTCGGGCAAGCTCTCCCTCATCGGCAATGGTGTCGTGTTCGATCCCCATGCCTTCGTG
GCGGAAATAGAGAGGCTGAAAGGGCAGGGCATCTCTGTGACGCCGGAAAGCCTGAAGGTCGCGGAAAACACGGCACTCAT
ACTGTCGCTGCACAGGGAACTCGACGCGTTCCGGGAGGATGCCGCCTCAAATTCGGGAACCAAGATCGGGACCACCCGCC
GCGGCATCGGTCCGGCCTATGAAGACAAGGTAGGACGGCGCGCCATCCGCGTCATGGATCTTGCCGACCCGGAGACACTA
TCGCTCAAGGTGGACCGGCTGCTGACCCATCACAATGCGCTTCGCCGCGGCCTGGGGCACGCCGAAGTCTCCCACGAGGC
CATCATGCAGGAGCTGACCTCGGTGGCCGACAAGATCCTGCCCTTCGTCGACCGGGTGTGGAAAATACTTGAGGATGCCC
GCCGCGCCGGCAGCCGCATCCTCTTTGAGGGTGCCCAAGGCACGATGCTCGATATCGATCATGGCACCTATCCGTTCGTG
ACCTCCTCGAACACGGTTGCTGGCCAGGCCGCCACCGGGTCCGGTCTGGGTCCCGGTGCGGTGGGCTATGTGCTTGGCAT
TACAAAAGCCTATACGACGCGCGTGGGCGAAGGGCCCTTCCCGACCGAGCAGGACAACGAGATCGGTGAATTTCTGGGCA
ACCGCGGACATGAATTCGGGACCGTCACCGGCCGTAAACGCCGCTGCGGTTGGTTCGATGCGGTGCAGGTGCGCCAGTCC
GTGGTGACAAACGGCATGAGCGGCATCGCCCTCACCAAGCTCGACGTGCTCGACGGGATGGAGGAGATAAAGGTCTGCAC
GGGCTACAGGCTGGACGGCCAGGTGATCGACTATCTTCCCGCGAGCCAAGGCGCGCAGGCGCGGCTCGACCCGATCTACG
AGACGCTGGAAGGCTGGAAGGAAACGACGCGCGGCGCCCGCAAATGGAATGATTTGCCTGCGCAGGCGGTGAAGTATGTC
CGCCATATCGAAGAGCTTATCGGCGCGCCGGTCGCGATTTTGTCGACCAGTCCGGAGCGGGAGGATGCGATCCTGGTAAA
GGACCCGTTCCAAGACTAG

Upstream 100 bases:

>100_bases
GGTGGTGAAAGAGGGGGTCGCATCGAGGACCGCATGGCGGTATAGAACCCCTGGTCTTGGTAAGGCTGGCGCGTTTGCCG
GCCCGTAGGAAGGAAGCGTT

Downstream 100 bases:

>100_bases
CGCATCGGCGTGACATCGGAATCGGTTCTCCGAATGCCGATGCGTAGACGATAATTTACAGCGTTCTTTGTGCGTCCTAA
AGGACGCACGGCGCTGTAGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGVSYKLSLLPSGVVRSGKLSLIGNGVVFDPHAFV
AEIERLKGQGISVTPESLKVAENTALILSLHRELDAFREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADPETL
SLKVDRLLTHHNALRRGLGHAEVSHEAIMQELTSVADKILPFVDRVWKILEDARRAGSRILFEGAQGTMLDIDHGTYPFV
TSSNTVAGQAATGSGLGPGAVGYVLGITKAYTTRVGEGPFPTEQDNEIGEFLGNRGHEFGTVTGRKRRCGWFDAVQVRQS
VVTNGMSGIALTKLDVLDGMEEIKVCTGYRLDGQVIDYLPASQGAQARLDPIYETLEGWKETTRGARKWNDLPAQAVKYV
RHIEELIGAPVAILSTSPEREDAILVKDPFQD

Sequences:

>Translated_432_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGVSYKLSLLPSGVVRSGKLSLIGNGVVFDPHAFV
AEIERLKGQGISVTPESLKVAENTALILSLHRELDAFREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADPETL
SLKVDRLLTHHNALRRGLGHAEVSHEAIMQELTSVADKILPFVDRVWKILEDARRAGSRILFEGAQGTMLDIDHGTYPFV
TSSNTVAGQAATGSGLGPGAVGYVLGITKAYTTRVGEGPFPTEQDNEIGEFLGNRGHEFGTVTGRKRRCGWFDAVQVRQS
VVTNGMSGIALTKLDVLDGMEEIKVCTGYRLDGQVIDYLPASQGAQARLDPIYETLEGWKETTRGARKWNDLPAQAVKYV
RHIEELIGAPVAILSTSPEREDAILVKDPFQD
>Mature_431_residues
ANVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGVSYKLSLLPSGVVRSGKLSLIGNGVVFDPHAFVA
EIERLKGQGISVTPESLKVAENTALILSLHRELDAFREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADPETLS
LKVDRLLTHHNALRRGLGHAEVSHEAIMQELTSVADKILPFVDRVWKILEDARRAGSRILFEGAQGTMLDIDHGTYPFVT
SSNTVAGQAATGSGLGPGAVGYVLGITKAYTTRVGEGPFPTEQDNEIGEFLGNRGHEFGTVTGRKRRCGWFDAVQVRQSV
VTNGMSGIALTKLDVLDGMEEIKVCTGYRLDGQVIDYLPASQGAQARLDPIYETLEGWKETTRGARKWNDLPAQAVKYVR
HIEELIGAPVAILSTSPEREDAILVKDPFQD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=44.4444444444444, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=44, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=42.8934010152284, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=53.2558139534884, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=44.0559440559441, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=44.0559440559441, Blast_Score=342, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=43.0555555555556, Blast_Score=342, Evalue=9e-95,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=42.4528301886792, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_MESSB (Q11DJ4)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_675696.1
- ProteinModelPortal:   Q11DJ4
- SMR:   Q11DJ4
- STRING:   Q11DJ4
- GeneID:   4181819
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_3159
- NMPDR:   fig|266779.1.peg.126
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q11DJ4
- ProtClustDB:   PRK01117
- BioCyc:   MSP266779:MESO_3159-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46832; Mature: 46700

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 132-132 BINDING 146-146 BINDING 226-226 BINDING 241-241 BINDING 305-305 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGVSYKLSLLPSGVV
CCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCCCC
RSGKLSLIGNGVVFDPHAFVAEIERLKGQGISVTPESLKVAENTALILSLHRELDAFRED
CCCCEEEEECCEEECCHHHHHHHHHHCCCCCEECHHHHHHHCCCCHHHHHHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADPETLSLKVDRLLTHHNALRRGLGH
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCC
AEVSHEAIMQELTSVADKILPFVDRVWKILEDARRAGSRILFEGAQGTMLDIDHGTYPFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEE
TSSNTVAGQAATGSGLGPGAVGYVLGITKAYTTRVGEGPFPTEQDNEIGEFLGNRGHEFG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCC
TVTGRKRRCGWFDAVQVRQSVVTNGMSGIALTKLDVLDGMEEIKVCTGYRLDGQVIDYLP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHCCCCCCCCCEECCC
ASQGAQARLDPIYETLEGWKETTRGARKWNDLPAQAVKYVRHIEELIGAPVAILSTSPER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
EDAILVKDPFQD
CCEEEECCCCCC
>Mature Secondary Structure 
ANVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGVSYKLSLLPSGVV
CCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCCCC
RSGKLSLIGNGVVFDPHAFVAEIERLKGQGISVTPESLKVAENTALILSLHRELDAFRED
CCCCEEEEECCEEECCHHHHHHHHHHCCCCCEECHHHHHHHCCCCHHHHHHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADPETLSLKVDRLLTHHNALRRGLGH
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCC
AEVSHEAIMQELTSVADKILPFVDRVWKILEDARRAGSRILFEGAQGTMLDIDHGTYPFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEE
TSSNTVAGQAATGSGLGPGAVGYVLGITKAYTTRVGEGPFPTEQDNEIGEFLGNRGHEFG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCC
TVTGRKRRCGWFDAVQVRQSVVTNGMSGIALTKLDVLDGMEEIKVCTGYRLDGQVIDYLP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHCCCCCCCCCEECCC
ASQGAQARLDPIYETLEGWKETTRGARKWNDLPAQAVKYVRHIEELIGAPVAILSTSPER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
EDAILVKDPFQD
CCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA