BacMap

Definition	Mesorhizobium sp. BNC1, complete genome.
Accession	NC_008254
Length	4,412,446

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The map label for this gene is dkgB [H]

Identifier: 110635381

GI number: 110635381

Start: 3326959

End: 3327783

Strand: Reverse

Name: dkgB [H]

Synonym: Meso_3052

Alternate gene names: 110635381

Gene position: 3327783-3326959 (Counterclockwise)

Preceding gene: 110635383

Following gene: 110635380

Centisome position: 75.42

GC content: 62.91

Gene sequence:

>825_bases
ATGCAAACCGTCACCGCTCACGGCGCGACCATCCCTGCTCTCGGCTTCGGCGTCTTCCGAATGTCCGACGCAGAGGTGGA
GCGCATTATCCCGGCCGCGCTCGAGGCCGGCTTCCGCCACTTCGACACTGCTCAAATCTACCAGAACGAAGCCGCGCTCG
GCCGGGCGCTGGAGCGTGCCGGTGCCCGCCGCGAGGATTTGTTTCTGACCACCAAGGTATGGGTCGATAACTATAGCGCC
GACAAGTTCGCCGCTTCTGTCGACGAAAGCCTCGAAAAGCTGAAGGTCGATCAGGTTGATCTCCTTCTCCTGCATTGGCC
GGCCCATGAGGTTCCGGTGGCGGCGCAGATCGAGAGGTTGAATGCCGTGAAGGACGCCGGAAAGACCCGGTTTATCGGCG
TATCCAACCAGAACATCGCGCAGATGCGGGAGTCGATCGCCCGCTCGGCCGCGCCGATCGTCACCAACCAGGTGGAATTG
CATCCCTATCTGCCCCAGCACTCGCTTGTCGCGGCCGCAAGGGACAGCGCTGTCGCAGTCACCGCCTATTACGGCATGGC
CGACGGCGCTGTGCCGAAGGACCCGGTACTGCAGCAGATCGGCGCCAGATACGGCAAGAGCGCCGCACAGGTTGGGCTAC
GCTGGCTTGTCCAGCAGGGCTATGTCGCCCTTTCCAAAACGGCTAACCCTGCACGCGTCGCGGAAAACATCGCGATCTTC
GATTTCGCCCTTGATGAAACCGATATGGCCGCCATCGCCAAGCTTGCCCGCCCCGACGGGCGGCTCGTCAGCCCTCCCGG
CCTTGCTCCTGATTGGGACGCCTAA

Upstream 100 bases:

>100_bases
ACAAACGCGTCCGCGCTCGATTCCACCCCGCATTCCCGGCGAATGGCGCTCGGGATCCGACACGGCAAGGCCGTTTGACC
AATCCAGAAGGACTTTCGCA

Downstream 100 bases:

>100_bases
GGAGCTTCAATCATGAACGCCAATGTCTCGACGGCCGGAACGACGGCCGAAGCATCCTCGTCCCCCCGCTGGGCGCTTCT
CGCTCTTGCGATCGGCGCTT

Product: aldo/keto reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERAGARREDLFLTTKVWVDNYSA
DKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERLNAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVEL
HPYLPQHSLVAAARDSAVAVTAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF
DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA

Sequences:

>Translated_274_residues
MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERAGARREDLFLTTKVWVDNYSA
DKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERLNAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVEL
HPYLPQHSLVAAARDSAVAVTAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF
DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA
>Mature_274_residues
MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERAGARREDLFLTTKVWVDNYSA
DKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERLNAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVEL
HPYLPQHSLVAAARDSAVAVTAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF
DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=284, Percent_Identity=29.5774647887324, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI24497585, Length=289, Percent_Identity=31.8339100346021, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI5174695, Length=290, Percent_Identity=31.3793103448276, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=30.8474576271186, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI5453543, Length=289, Percent_Identity=30.7958477508651, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI310109922, Length=270, Percent_Identity=31.4814814814815, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=30.4498269896194, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=30.1038062283737, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=30.1038062283737, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI24497583, Length=292, Percent_Identity=30.8219178082192, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=29.7945205479452, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=29.7945205479452, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI291291012, Length=269, Percent_Identity=28.6245353159851, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4502049, Length=287, Percent_Identity=29.9651567944251, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI300116273, Length=272, Percent_Identity=30.1470588235294, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI300116271, Length=269, Percent_Identity=30.1115241635688, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI310109924, Length=151, Percent_Identity=31.1258278145695, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310109926, Length=107, Percent_Identity=35.5140186915888, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI207028673, Length=107, Percent_Identity=34.5794392523364, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI27436969, Length=290, Percent_Identity=23.7931034482759, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4504825, Length=290, Percent_Identity=23.7931034482759, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1786400, Length=266, Percent_Identity=43.609022556391, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI87082198, Length=261, Percent_Identity=35.2490421455939, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1788081, Length=265, Percent_Identity=32.0754716981132, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87081735, Length=144, Percent_Identity=35.4166666666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788070, Length=312, Percent_Identity=24.3589743589744, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17550248, Length=279, Percent_Identity=34.0501792114695, Blast_Score=153, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17537075, Length=292, Percent_Identity=32.1917808219178, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17537077, Length=279, Percent_Identity=31.8996415770609, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=31.5985130111524, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17564128, Length=284, Percent_Identity=31.6901408450704, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17566692, Length=278, Percent_Identity=32.0143884892086, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17552492, Length=258, Percent_Identity=32.1705426356589, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=29.368029739777, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17537079, Length=303, Percent_Identity=29.7029702970297, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17561300, Length=266, Percent_Identity=28.9473684210526, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=30.03663003663, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17562292, Length=288, Percent_Identity=26.7361111111111, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320576, Length=285, Percent_Identity=34.7368421052632, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6324694, Length=291, Percent_Identity=34.020618556701, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319625, Length=279, Percent_Identity=30.4659498207885, Blast_Score=128, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6322556, Length=265, Percent_Identity=26.7924528301887, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320079, Length=249, Percent_Identity=28.5140562248996, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24657054, Length=277, Percent_Identity=32.8519855595668, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21356425, Length=278, Percent_Identity=33.8129496402878, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24644950, Length=290, Percent_Identity=34.1379310344828, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221378297, Length=310, Percent_Identity=31.2903225806452, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24663317, Length=281, Percent_Identity=32.0284697508897, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=30.5084745762712, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI20129731, Length=274, Percent_Identity=32.4817518248175, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI45553081, Length=275, Percent_Identity=30.9090909090909, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24662785, Length=295, Percent_Identity=29.4915254237288, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=28.6206896551724, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI281366140, Length=240, Percent_Identity=31.6666666666667, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29639; Mature: 29639

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERA
CCEEECCCCCCHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GARREDLFLTTKVWVDNYSADKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERL
CCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
NAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVELHPYLPQHSLVAAARDSAVAV
HHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEECEEEECCCCCCHHHHHHHCCCEEEE
TAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF
EEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHCEEEE
DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA
EEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCC
>Mature Secondary Structure
MQTVTAHGATIPALGFGVFRMSDAEVERIIPAALEAGFRHFDTAQIYQNEAALGRALERA
CCEEECCCCCCHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GARREDLFLTTKVWVDNYSADKFAASVDESLEKLKVDQVDLLLLHWPAHEVPVAAQIERL
CCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
NAVKDAGKTRFIGVSNQNIAQMRESIARSAAPIVTNQVELHPYLPQHSLVAAARDSAVAV
HHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEECEEEECCCCCCHHHHHHHCCCEEEE
TAYYGMADGAVPKDPVLQQIGARYGKSAAQVGLRWLVQQGYVALSKTANPARVAENIAIF
EEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHCEEEE
DFALDETDMAAIAKLARPDGRLVSPPGLAPDWDA
EEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]