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The map label for this gene is mdlB [H]

Identifier: 110635374

GI number: 110635374

Start: 3319322

End: 3320446

Strand: Reverse

Name: mdlB [H]

Synonym: Meso_3045

Alternate gene names: 110635374

Gene position: 3320446-3319322 (Counterclockwise)

Preceding gene: 110635375

Following gene: 110635373

Centisome position: 75.25

GC content: 64.98

Gene sequence:

>1125_bases
ATGCGCTACCGGTCGAAAGCGCCTCTGGTCTGTGCGGATGATCTGCGGGAAGCAGCCCGCCGGCGCATGCCGCGCTTCGC
GTTCGATTTCATCGACGGGGCGGCGGGAAGCGAGGCGGGACTGGCACGCAACCGAGCAGCCTTCGAGGCGGAGCTGCTCA
GGCCACGCGCGCTGGTCAATACCGATGGCGACCTGAAGACCGGGAGGCGTTTCCTGGGGCAGGAATGGTCGGTTCCGTTC
GGCATCTCGCCCATTGGGCTGGCTGGCATCGCCTGGCCGGGAATCGATCTGGTCCTGGCTGGTGCGGCGGAGCGCAACGG
TGCGCCTTATATTGCCTCCACGCCGGCCACCGCCAGCCTTGAGGATCTCAAACGCACGGCAGCCGTTTCCGCCTGGTTCC
AGCTTTATGTCGGCCGTTCACAGGAGATCGTGGATGACCTGATCGACCGGGCCGCGGCCGCGGGTTACGAGGTTCTGGTT
GTCACAGTCGACGTGCCGCGTCCGGGCAAGCGCCGGCGGGACCTGCGCAACGGCTTTGCCCTGCCGTTGAAGATCGGCCC
GCGTATGATCGCGGATCTCATGACGCATCCGAAGTGGTCGCTTGAAGTTCTGCGTAAGGGCGCGCCCCGCTTCGCCAACC
TGGAACGTTATGCCCCTCCCGGTGCCGGCGCGCGGTCCCTTGCGGAGTTGATGGCCGGGCAGAGCTCCGGGCGGCTTGAT
TGGACGCTTTTGGCGCAGCTACGCGAGCGCTGGCAAGGACGGCTGGTCGTCAAGGGCATCATGGCACCCGAGGACGCTAT
GCGTGCTCGCGAAGAGGGGGTGGATGCGGTAATCGTGTCCAATCATGGAGGGCGCCAGCTTGATAGCGCGCCGGCCACCC
TGCATGCGCTGAAGCAAATCCGCGAAGCCGTCGGGCCGGATTATTCGCTGGCGGTTGATGGCGGGATCCGCACCGGCGAG
GACATCCTGAAAAGCCTATTCGCCGGCGCGGATTTCACTTTTCTCGGGCGTCCGTTCCTCTATGCCGTTGCAGCGCGGGG
GACGAATGGGGCGAACGATCTCTTTGACATGCTCCGCGCAGAACTCGTAAACGCCATGGCACAGGTCGGGCGTTGTGAAC
TGTGA

Upstream 100 bases:

>100_bases
GAAATTACCGGACACACCGGGGCTGGGTTTCGCAGCGGATCCGGCTGCATTGCGCGAGTTTGCGGCACGGCCGACATCGC
GCGGAAAAGGAAAGGGCTGA

Downstream 100 bases:

>100_bases
CTGTTTTCGCTGTTTTGAGAGGCGTTTTGCGGGCACGTTGTTTGAGACTGACTGAGAGGTTTGCCGGATGACCCGTCCAG
ATGATGTCGTGGAGCAAGCG

Product: FMN-dependent alpha-hydroxy acid dehydrogenase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRYRSKAPLVCADDLREAARRRMPRFAFDFIDGAAGSEAGLARNRAAFEAELLRPRALVNTDGDLKTGRRFLGQEWSVPF
GISPIGLAGIAWPGIDLVLAGAAERNGAPYIASTPATASLEDLKRTAAVSAWFQLYVGRSQEIVDDLIDRAAAAGYEVLV
VTVDVPRPGKRRRDLRNGFALPLKIGPRMIADLMTHPKWSLEVLRKGAPRFANLERYAPPGAGARSLAELMAGQSSGRLD
WTLLAQLRERWQGRLVVKGIMAPEDAMRAREEGVDAVIVSNHGGRQLDSAPATLHALKQIREAVGPDYSLAVDGGIRTGE
DILKSLFAGADFTFLGRPFLYAVAARGTNGANDLFDMLRAELVNAMAQVGRCEL

Sequences:

>Translated_374_residues
MRYRSKAPLVCADDLREAARRRMPRFAFDFIDGAAGSEAGLARNRAAFEAELLRPRALVNTDGDLKTGRRFLGQEWSVPF
GISPIGLAGIAWPGIDLVLAGAAERNGAPYIASTPATASLEDLKRTAAVSAWFQLYVGRSQEIVDDLIDRAAAAGYEVLV
VTVDVPRPGKRRRDLRNGFALPLKIGPRMIADLMTHPKWSLEVLRKGAPRFANLERYAPPGAGARSLAELMAGQSSGRLD
WTLLAQLRERWQGRLVVKGIMAPEDAMRAREEGVDAVIVSNHGGRQLDSAPATLHALKQIREAVGPDYSLAVDGGIRTGE
DILKSLFAGADFTFLGRPFLYAVAARGTNGANDLFDMLRAELVNAMAQVGRCEL
>Mature_374_residues
MRYRSKAPLVCADDLREAARRRMPRFAFDFIDGAAGSEAGLARNRAAFEAELLRPRALVNTDGDLKTGRRFLGQEWSVPF
GISPIGLAGIAWPGIDLVLAGAAERNGAPYIASTPATASLEDLKRTAAVSAWFQLYVGRSQEIVDDLIDRAAAAGYEVLV
VTVDVPRPGKRRRDLRNGFALPLKIGPRMIADLMTHPKWSLEVLRKGAPRFANLERYAPPGAGARSLAELMAGQSSGRLD
WTLLAQLRERWQGRLVVKGIMAPEDAMRAREEGVDAVIVSNHGGRQLDSAPATLHALKQIREAVGPDYSLAVDGGIRTGE
DILKSLFAGADFTFLGRPFLYAVAARGTNGANDLFDMLRAELVNAMAQVGRCEL

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI54234014, Length=363, Percent_Identity=34.435261707989, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI7705393, Length=363, Percent_Identity=34.435261707989, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI11068137, Length=367, Percent_Identity=32.1525885558583, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI1790033, Length=363, Percent_Identity=35.8126721763085, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI193208036, Length=371, Percent_Identity=33.9622641509434, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI32566217, Length=295, Percent_Identity=33.8983050847458, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6323587, Length=369, Percent_Identity=29.810298102981, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI281363140, Length=377, Percent_Identity=33.6870026525199, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI78707190, Length=377, Percent_Identity=33.6870026525199, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI78707188, Length=371, Percent_Identity=33.9622641509434, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 40575; Mature: 40575

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYRSKAPLVCADDLREAARRRMPRFAFDFIDGAAGSEAGLARNRAAFEAELLRPRALVN
CCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHEEC
TDGDLKTGRRFLGQEWSVPFGISPIGLAGIAWPGIDLVLAGAAERNGAPYIASTPATASL
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHEEEECCCCCCCCCEEECCCCCCCH
EDLKRTAAVSAWFQLYVGRSQEIVDDLIDRAAAAGYEVLVVTVDVPRPGKRRRDLRNGFA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCE
LPLKIGPRMIADLMTHPKWSLEVLRKGAPRFANLERYAPPGAGARSLAELMAGQSSGRLD
EEEECCHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCH
WTLLAQLRERWQGRLVVKGIMAPEDAMRAREEGVDAVIVSNHGGRQLDSAPATLHALKQI
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHH
REAVGPDYSLAVDGGIRTGEDILKSLFAGADFTFLGRPFLYAVAARGTNGANDLFDMLRA
HHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
ELVNAMAQVGRCEL
HHHHHHHHHCCCCC
>Mature Secondary Structure
MRYRSKAPLVCADDLREAARRRMPRFAFDFIDGAAGSEAGLARNRAAFEAELLRPRALVN
CCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHEEC
TDGDLKTGRRFLGQEWSVPFGISPIGLAGIAWPGIDLVLAGAAERNGAPYIASTPATASL
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHEEEECCCCCCCCCEEECCCCCCCH
EDLKRTAAVSAWFQLYVGRSQEIVDDLIDRAAAAGYEVLVVTVDVPRPGKRRRDLRNGFA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCE
LPLKIGPRMIADLMTHPKWSLEVLRKGAPRFANLERYAPPGAGARSLAELMAGQSSGRLD
EEEECCHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCH
WTLLAQLRERWQGRLVVKGIMAPEDAMRAREEGVDAVIVSNHGGRQLDSAPATLHALKQI
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHH
REAVGPDYSLAVDGGIRTGEDILKSLFAGADFTFLGRPFLYAVAARGTNGANDLFDMLRA
HHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
ELVNAMAQVGRCEL
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]