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The map label for this gene is metE

Identifier: 110635035

GI number: 110635035

Start: 2934883

End: 2937192

Strand: Reverse

Name: metE

Synonym: Meso_2701

Alternate gene names: 110635035

Gene position: 2937192-2934883 (Counterclockwise)

Preceding gene: 110635036

Following gene: 110635034

Centisome position: 66.57

GC content: 64.29

Gene sequence:

>2310_bases
ATGTCACAAATACCCGTTGCCACGCTCGGCGTGCCACGTATAGGCCGCCGCCGTGAACTCAAATTCGCGCTGGAAGCATA
TTGGGGGGGCGAAAGCTCGGAGGGCGAGCTTCTTGCCGCTGCCGCGAAGATCCGCGCTGAAAACTGGTCTCTGCAGAAGG
CGGCAGGCGCCAGCATCATCCCGTCCAACGATTTCACGCTTTACGACCACGTGCTGGATATGGCCGTGACGCTGGGCGCC
ATTCCTGAAATCTACGGCTGGGCCGGCGGCGCTGTGCCGCTCGACACCTATTTCGCCATGGCGCGCGGCAGCCAGGAAAG
AGAGGCTTGCGGCCATTCGCATCATGACGGCACCGGTGTGCCGGCGCTGGAGATGACGAAGTGGTTCGATACGAACTATC
ACTATATGGTGCCGGAATTCGATGCCGATCAGACGTTCCGGCTCGCCTCGCTGAAGCCGGTCGAGGAGTTCGACGAGGCA
AAGCGGCAGGGAATCCACACGCGGCCGGTGCTGCTGGGGCCGGTCTCTTTTCTCAAGCTCGGCAAGCGCAGGGACGGTGG
CGACCCGCTGGAGCTCCTGCCGCGGCTGGTGCCGGTCTACGCGGAATTGCTGGGCAAGCTGCTGCGGGCGGGCGCGGACT
GGGTTCAGATCGATGAACCCTGCCTCGTGCTCGACCTTACGGAAAGGGAGCGGCGAGCTTTCACCATCGCCTATGACGCG
CTGGCGGCGACCGGAGGTCCCAAGCTGATGCTCGCCACCTATTTCGGCGGGCTGGGCGACAATCTGCCAACGGCAGCCGC
CCTGCCGGTCGCCGGGCTGCACCTCGACCTCGTGCGCGCGCCGGATCAGCTTCAAGATGTGCTTTCGGCGGCGCCGGAGG
AGCGCGTGATCTCGTTGGGTGTCGTGGATGGCCGCAACATCTGGCGCACGGACATTGCTGCACTTCTCGACAGGCTGGGT
CCGATCGTGAAGGGCAATCCCGGTCGATTCCAGTTAGCGCCGTCCTGTTCCCTCCTTCATGTGCCCCTGGATCTCTCGAT
CGAAACCGGGGTCGATCCGGAGCTCAGGAGCTGGCTTGCCTTCGCCGTGCAGAAGGTGGAAGAACTCTCCATTCTCTCCC
ACGCGCTGTCGGCCGACCGCTCGCTGGTTCAGTCCCTCATCGACATGGCTTCACGGGCGCTTGCCGTGCGGGCTTCTTCG
ACCCGCGTGAATGATCCGCATGTCGCTGGCCGGCTCTCCGCCGTCACGCCCGAGATGGCGAAGCGTTCCGAGCCGTTTTC
CGAGCGGCGCAAGCTGCAGCAGGAGCGGCTGAACCTGCCGGCATTCCCGACCACGACCATCGGCTCCTTCCCGCAGACGG
CGGAGGTGCGCCAGGCGCGCGCCGCCTGTGCGAAGGGGGAGCTCAGCGAAGCCGGTTATGATGGCTTCCTCAAGGGGGAG
ACGGAGAAGGCGATCCGGTGGCAAGAGGAGATCGGCATCGATGTGCTCGTGCATGGCGAGTTCGAGCGCAACGATATGGT
GCAGTATTTCAGCGAGCAGCTTTCGGGCTATGCCTTCACCAAGCAGGGCTGGGTCCAGAGCTATGGTTCGCGCTGCGTCC
GCCCGCCCATCATCTTCGGTGACGTTTCCCGGCCGCAGCCGATGACGGTCGATTGGGCCACCTATGCCCAGTCGCTCACC
GACAAGCCGGTGAAGGGTATGCTCACGGGACCCGTCACGATGCTGCAATGGTCGTTCGTGCGTGACGACCTGCCGCGCGA
GACGGTGTGCCGCCAGATCGCATTTGCGTTGCGAGACGAAGTGAGCGACCTCGAAGCAGCGGGGATCGGGGTTATCCAGA
TCGACGAGCCGGCGCTGCGTGAAGGCCTGCCGCTGCGGGAAGCCGAGCGGCCGGCCTATCTCGACTGGGCAGTGGAGTGC
TTCCGTCTGGCCTCCTCCGGCGTGAAGCCCGAGACCCAGATACACACCCACATGTGCTACGCGGAGTTCAACGACATCAT
CGATGCGATCGCGGCGATGGATGCGGATGTGATTTCCATCGAAACCTCGCGATCCAAGATGGAGCTTCTGCGAGCGTTTC
GCGCGTTCCGCTATCCCAACGATATCGGACCCGGCGTCTTCGACATCCATTCGCCGCGGGTGCCGCCCGCCGACGAGATG
GCCGACCTCCTGCGGAAGGCGGAGGACGGGCTGGATCCGCGCCAGATCTGGGTCAATCCCGATTGCGGGCTGAAGACCCG
CAAATGGGAGGAAGTCCGCTTCGCGCTTGCCAATATGGTGGAGGCAGCGCGGCAGATGCGGGCGGGCTGA

Upstream 100 bases:

>100_bases
ACGTCCACGGGCGGGGTGTCCCGGTGGCCGCAGCGAGGCGGCGCGTGCCGCTTTCGCGGGCGTCCGCGTGCCCATGCCCC
CTAATGAAAGGGGTGAAACC

Downstream 100 bases:

>100_bases
TGAAAATCGGCGTTCCCGCACCAACTCCCTTCCGGCGCGGGAACGCCGCCACAACGCCGGCGTTTCTGCCCCGAAAGGGT
GCAGCCATGGCCGGACGGCA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 769; Mature: 768

Protein sequence:

>769_residues
MSQIPVATLGVPRIGRRRELKFALEAYWGGESSEGELLAAAAKIRAENWSLQKAAGASIIPSNDFTLYDHVLDMAVTLGA
IPEIYGWAGGAVPLDTYFAMARGSQEREACGHSHHDGTGVPALEMTKWFDTNYHYMVPEFDADQTFRLASLKPVEEFDEA
KRQGIHTRPVLLGPVSFLKLGKRRDGGDPLELLPRLVPVYAELLGKLLRAGADWVQIDEPCLVLDLTERERRAFTIAYDA
LAATGGPKLMLATYFGGLGDNLPTAAALPVAGLHLDLVRAPDQLQDVLSAAPEERVISLGVVDGRNIWRTDIAALLDRLG
PIVKGNPGRFQLAPSCSLLHVPLDLSIETGVDPELRSWLAFAVQKVEELSILSHALSADRSLVQSLIDMASRALAVRASS
TRVNDPHVAGRLSAVTPEMAKRSEPFSERRKLQQERLNLPAFPTTTIGSFPQTAEVRQARAACAKGELSEAGYDGFLKGE
TEKAIRWQEEIGIDVLVHGEFERNDMVQYFSEQLSGYAFTKQGWVQSYGSRCVRPPIIFGDVSRPQPMTVDWATYAQSLT
DKPVKGMLTGPVTMLQWSFVRDDLPRETVCRQIAFALRDEVSDLEAAGIGVIQIDEPALREGLPLREAERPAYLDWAVEC
FRLASSGVKPETQIHTHMCYAEFNDIIDAIAAMDADVISIETSRSKMELLRAFRAFRYPNDIGPGVFDIHSPRVPPADEM
ADLLRKAEDGLDPRQIWVNPDCGLKTRKWEEVRFALANMVEAARQMRAG

Sequences:

>Translated_769_residues
MSQIPVATLGVPRIGRRRELKFALEAYWGGESSEGELLAAAAKIRAENWSLQKAAGASIIPSNDFTLYDHVLDMAVTLGA
IPEIYGWAGGAVPLDTYFAMARGSQEREACGHSHHDGTGVPALEMTKWFDTNYHYMVPEFDADQTFRLASLKPVEEFDEA
KRQGIHTRPVLLGPVSFLKLGKRRDGGDPLELLPRLVPVYAELLGKLLRAGADWVQIDEPCLVLDLTERERRAFTIAYDA
LAATGGPKLMLATYFGGLGDNLPTAAALPVAGLHLDLVRAPDQLQDVLSAAPEERVISLGVVDGRNIWRTDIAALLDRLG
PIVKGNPGRFQLAPSCSLLHVPLDLSIETGVDPELRSWLAFAVQKVEELSILSHALSADRSLVQSLIDMASRALAVRASS
TRVNDPHVAGRLSAVTPEMAKRSEPFSERRKLQQERLNLPAFPTTTIGSFPQTAEVRQARAACAKGELSEAGYDGFLKGE
TEKAIRWQEEIGIDVLVHGEFERNDMVQYFSEQLSGYAFTKQGWVQSYGSRCVRPPIIFGDVSRPQPMTVDWATYAQSLT
DKPVKGMLTGPVTMLQWSFVRDDLPRETVCRQIAFALRDEVSDLEAAGIGVIQIDEPALREGLPLREAERPAYLDWAVEC
FRLASSGVKPETQIHTHMCYAEFNDIIDAIAAMDADVISIETSRSKMELLRAFRAFRYPNDIGPGVFDIHSPRVPPADEM
ADLLRKAEDGLDPRQIWVNPDCGLKTRKWEEVRFALANMVEAARQMRAG
>Mature_768_residues
SQIPVATLGVPRIGRRRELKFALEAYWGGESSEGELLAAAAKIRAENWSLQKAAGASIIPSNDFTLYDHVLDMAVTLGAI
PEIYGWAGGAVPLDTYFAMARGSQEREACGHSHHDGTGVPALEMTKWFDTNYHYMVPEFDADQTFRLASLKPVEEFDEAK
RQGIHTRPVLLGPVSFLKLGKRRDGGDPLELLPRLVPVYAELLGKLLRAGADWVQIDEPCLVLDLTERERRAFTIAYDAL
AATGGPKLMLATYFGGLGDNLPTAAALPVAGLHLDLVRAPDQLQDVLSAAPEERVISLGVVDGRNIWRTDIAALLDRLGP
IVKGNPGRFQLAPSCSLLHVPLDLSIETGVDPELRSWLAFAVQKVEELSILSHALSADRSLVQSLIDMASRALAVRASST
RVNDPHVAGRLSAVTPEMAKRSEPFSERRKLQQERLNLPAFPTTTIGSFPQTAEVRQARAACAKGELSEAGYDGFLKGET
EKAIRWQEEIGIDVLVHGEFERNDMVQYFSEQLSGYAFTKQGWVQSYGSRCVRPPIIFGDVSRPQPMTVDWATYAQSLTD
KPVKGMLTGPVTMLQWSFVRDDLPRETVCRQIAFALRDEVSDLEAAGIGVIQIDEPALREGLPLREAERPAYLDWAVECF
RLASSGVKPETQIHTHMCYAEFNDIIDAIAAMDADVISIETSRSKMELLRAFRAFRYPNDIGPGVFDIHSPRVPPADEMA
DLLRKAEDGLDPRQIWVNPDCGLKTRKWEEVRFALANMVEAARQMRAG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=765, Percent_Identity=52.9411764705882, Blast_Score=794, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=770, Percent_Identity=50.5194805194805, Blast_Score=748, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_MESSB (Q11EU7)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_675243.1
- ProteinModelPortal:   Q11EU7
- SMR:   Q11EU7
- STRING:   Q11EU7
- GeneID:   4181727
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_2701
- NMPDR:   fig|266779.1.peg.1666
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- PhylomeDB:   Q11EU7
- ProtClustDB:   PRK05222
- BioCyc:   MSP266779:MESO_2701-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 84936; Mature: 84805

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIPVATLGVPRIGRRRELKFALEAYWGGESSEGELLAAAAKIRAENWSLQKAAGASII
CCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCHHHHCCCCCC
PSNDFTLYDHVLDMAVTLGAIPEIYGWAGGAVPLDTYFAMARGSQEREACGHSHHDGTGV
CCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCC
PALEMTKWFDTNYHYMVPEFDADQTFRLASLKPVEEFDEAKRQGIHTRPVLLGPVSFLKL
CHHHHHHHCCCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHH
GKRRDGGDPLELLPRLVPVYAELLGKLLRAGADWVQIDEPCLVLDLTERERRAFTIAYDA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEEEEEHH
LAATGGPKLMLATYFGGLGDNLPTAAALPVAGLHLDLVRAPDQLQDVLSAAPEERVISLG
HHCCCCCCEEHEEHHCCCCCCCCCHHHCCCCCCEEHHHCCCHHHHHHHHCCCCHHEEEEE
VVDGRNIWRTDIAALLDRLGPIVKGNPGRFQLAPSCSLLHVPLDLSIETGVDPELRSWLA
EECCCCHHHHHHHHHHHHCCCCEECCCCCEEECCCCEEEEECEEEEECCCCCHHHHHHHH
FAVQKVEELSILSHALSADRSLVQSLIDMASRALAVRASSTRVNDPHVAGRLSAVTPEMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCHHHHCCHHHH
KRSEPFSERRKLQQERLNLPAFPTTTIGSFPQTAEVRQARAACAKGELSEAGYDGFLKGE
HCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TEKAIRWQEEIGIDVLVHGEFERNDMVQYFSEQLSGYAFTKQGWVQSYGSRCVRPPIIFG
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCEEEC
DVSRPQPMTVDWATYAQSLTDKPVKGMLTGPVTMLQWSFVRDDLPRETVCRQIAFALRDE
CCCCCCCCEEEHHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VSDLEAAGIGVIQIDEPALREGLPLREAERPAYLDWAVECFRLASSGVKPETQIHTHMCY
HHHHHHCCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AEFNDIIDAIAAMDADVISIETSRSKMELLRAFRAFRYPNDIGPGVFDIHSPRVPPADEM
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHH
ADLLRKAEDGLDPRQIWVNPDCGLKTRKWEEVRFALANMVEAARQMRAG
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQIPVATLGVPRIGRRRELKFALEAYWGGESSEGELLAAAAKIRAENWSLQKAAGASII
CCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCHHHHCCCCCC
PSNDFTLYDHVLDMAVTLGAIPEIYGWAGGAVPLDTYFAMARGSQEREACGHSHHDGTGV
CCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCC
PALEMTKWFDTNYHYMVPEFDADQTFRLASLKPVEEFDEAKRQGIHTRPVLLGPVSFLKL
CHHHHHHHCCCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHH
GKRRDGGDPLELLPRLVPVYAELLGKLLRAGADWVQIDEPCLVLDLTERERRAFTIAYDA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEEEEEHH
LAATGGPKLMLATYFGGLGDNLPTAAALPVAGLHLDLVRAPDQLQDVLSAAPEERVISLG
HHCCCCCCEEHEEHHCCCCCCCCCHHHCCCCCCEEHHHCCCHHHHHHHHCCCCHHEEEEE
VVDGRNIWRTDIAALLDRLGPIVKGNPGRFQLAPSCSLLHVPLDLSIETGVDPELRSWLA
EECCCCHHHHHHHHHHHHCCCCEECCCCCEEECCCCEEEEECEEEEECCCCCHHHHHHHH
FAVQKVEELSILSHALSADRSLVQSLIDMASRALAVRASSTRVNDPHVAGRLSAVTPEMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCHHHHCCHHHH
KRSEPFSERRKLQQERLNLPAFPTTTIGSFPQTAEVRQARAACAKGELSEAGYDGFLKGE
HCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TEKAIRWQEEIGIDVLVHGEFERNDMVQYFSEQLSGYAFTKQGWVQSYGSRCVRPPIIFG
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCEEEC
DVSRPQPMTVDWATYAQSLTDKPVKGMLTGPVTMLQWSFVRDDLPRETVCRQIAFALRDE
CCCCCCCCEEEHHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VSDLEAAGIGVIQIDEPALREGLPLREAERPAYLDWAVECFRLASSGVKPETQIHTHMCY
HHHHHHCCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AEFNDIIDAIAAMDADVISIETSRSKMELLRAFRAFRYPNDIGPGVFDIHSPRVPPADEM
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHH
ADLLRKAEDGLDPRQIWVNPDCGLKTRKWEEVRFALANMVEAARQMRAG
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA