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The map label for this gene is thiC [H]

Identifier: 110634902

GI number: 110634902

Start: 2750313

End: 2752139

Strand: Direct

Name: thiC [H]

Synonym: Meso_2561

Alternate gene names: 110634902

Gene position: 2750313-2752139 (Clockwise)

Preceding gene: 110634901

Following gene: 110634903

Centisome position: 62.33

GC content: 62.34

Gene sequence:

>1827_bases
ATGGATGCCCGGAACAACCCCAAGCCCGAGATTACAACAGGCCCCCTTCCCGCCTCCAGCAAGGTCTACAAGCCCGGCAC
GCTGCACTCTGAGATCCGCGTGCCGATGCGCGAGATCGCCGTCCACCCCACGGCGGGCGAACCGCCCGTTACGGTCTATG
ATTCTTCCGGACCCTACACGGATACGAGCGTCACCATCGACATCGAGAAGGGCCTGCCGCGCCTGCGCGAAACCTGGATC
GAGGCGCGTGGCGACGTGGAGCGCTATGACGGCCGCATCGTCAAGCCGGAGGACAACGGCTTCGTATCGGGCGAGCGGCT
GACGCCTGAATTCCCAGTCCGCAACCGTCCGCTTAAGGCGCGGGACGGCAAGGCAGTCACACAGCTTGCCTATGCGCGGG
CGGGCATTGTGACGCCGGAGATGGAGTTCATCGCCATTCGCGAAAATCTCGGGCGCGAAAAGCTGAAGGAAAAGCTCGTC
CGTGACGGTGAGAGCTTCGGCGCGGCGGTTCCCGACTATGTGACGCCGGAGTTCGTGCGAGACGAGGTGGCGCGCGGGCG
GGCCATCATTCCAGCCAATATCAACCACCCCGAATCCGAGCCGATGATCATCGGCCGCAATTTCCTGACCAAGATCAACG
CCAATATCGGCAATTCCGCTGTCACCTCCTCCATGGCCGAGGAGGTGGAAAAGCTGGTCTGGGCAACGCGATGGGGCGCG
GACACGGTTATGGATCTTTCGACCGGCCGCAACATCCACAATATCCGTGAATGGATCATCCGCAATTCCCCCGTACCCAT
CGGTACGGTGCCGATCTATCAGGCGCTGGAGAAGGTGGGCGGCATTGCCGAGGAGCTCACCTGGGAGGTCTTCCGCGATA
CGCTTATCGAGCAGGCCGAGCAGGGCGTGGATTATTTCACCATCCATGCCGGTGTCAGGCTCCACTATGTGCCGCTGACG
GTGAACCGCGTAACGGGCATTGTCTCGCGTGGCGGGTCCATCATGGCGAAGTGGTGCCTCGCTCACCATAAGGAGAGCTT
TCTCTACGAGCATTTCGCGGAGATCTGCGACATCTGCCGCGCCTATGACGTCTCCTTCTCGCTGGGCGACGGACTTCGCC
CCGGCTCGATCGCGGATGCCAATGACGCAGCGCAATTTGCCGAGTTGGAGACGCTGGGCGAGCTGACGAAAATCGCCTGG
GCCAAGGATTGCCAGGTGATGATCGAGGGACCCGGCCATGTTCCCATGCACAAGATCAAGGAGAACATGGACAAGCAGCT
CGAGATCTGCGGCGAGGCGCCATTCTATACGCTCGGGCCGCTCACAACCGACATCGCGCCGGGCTACGACCACATCACTT
CGGCCATCGGCGCCGCAATGATCGGCTGGTTCGGAACGGCCATGCTCTGTTACGTGACCCCCAAGGAGCATCTGGGCCTG
CCCGACCGTGATGACGTGAAGACCGGCGTCATCACTTACAAGATCGCGGCGCACGCCGCTGACCTGGCCAAGGGCCACCC
GGCGGCCAAGGTGAGGGACGACGCGTTGTCGCGCGCCCGTTTCGAATTCCGCTGGGAGGACCAGTTCAACCTCTCGCTCG
ACCCCGAAACGGCGCGCGCGCTACATGATGAGACCCTGCCCAAGGAGGCGCATAAAGTGGCGCATTTCTGCTCCATGTGC
GGCCCGAAATTCTGCTCCATGCGGATCTCGCACGACATTCGGGCCGAGGCGCAGAAGGAAGGCATGGCCGCAATGGCCGA
GAAGTACCGCGAGGGCGGGGATTTGTATGTGGCTGTGGAGGAGACGGCAGAGACGCAGGGCGCTTGA

Upstream 100 bases:

>100_bases
CGGTCGTAAGATCAAAGGGAATATCGTTTTTCCCGGCAAGCTTCTCCCCTCCTCCTTCGGCAAGAACTGGGTCTCCAACG
CGAACGCGGAGAGACTCGCC

Downstream 100 bases:

>100_bases
GGACAGGTGGATTTGCTATGCTTGGGAAAGCCGGTGGCCTTATACCCCCCTCTGCCCGGCCGGGCATCTCCCCCTCAAGG
GGGGAGATTGGGCCGGCGGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MDARNNPKPEITTGPLPASSKVYKPGTLHSEIRVPMREIAVHPTAGEPPVTVYDSSGPYTDTSVTIDIEKGLPRLRETWI
EARGDVERYDGRIVKPEDNGFVSGERLTPEFPVRNRPLKARDGKAVTQLAYARAGIVTPEMEFIAIRENLGREKLKEKLV
RDGESFGAAVPDYVTPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLTKINANIGNSAVTSSMAEEVEKLVWATRWGA
DTVMDLSTGRNIHNIREWIIRNSPVPIGTVPIYQALEKVGGIAEELTWEVFRDTLIEQAEQGVDYFTIHAGVRLHYVPLT
VNRVTGIVSRGGSIMAKWCLAHHKESFLYEHFAEICDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAW
AKDCQVMIEGPGHVPMHKIKENMDKQLEICGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
PDRDDVKTGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARALHDETLPKEAHKVAHFCSMC
GPKFCSMRISHDIRAEAQKEGMAAMAEKYREGGDLYVAVEETAETQGA

Sequences:

>Translated_608_residues
MDARNNPKPEITTGPLPASSKVYKPGTLHSEIRVPMREIAVHPTAGEPPVTVYDSSGPYTDTSVTIDIEKGLPRLRETWI
EARGDVERYDGRIVKPEDNGFVSGERLTPEFPVRNRPLKARDGKAVTQLAYARAGIVTPEMEFIAIRENLGREKLKEKLV
RDGESFGAAVPDYVTPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLTKINANIGNSAVTSSMAEEVEKLVWATRWGA
DTVMDLSTGRNIHNIREWIIRNSPVPIGTVPIYQALEKVGGIAEELTWEVFRDTLIEQAEQGVDYFTIHAGVRLHYVPLT
VNRVTGIVSRGGSIMAKWCLAHHKESFLYEHFAEICDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAW
AKDCQVMIEGPGHVPMHKIKENMDKQLEICGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
PDRDDVKTGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARALHDETLPKEAHKVAHFCSMC
GPKFCSMRISHDIRAEAQKEGMAAMAEKYREGGDLYVAVEETAETQGA
>Mature_608_residues
MDARNNPKPEITTGPLPASSKVYKPGTLHSEIRVPMREIAVHPTAGEPPVTVYDSSGPYTDTSVTIDIEKGLPRLRETWI
EARGDVERYDGRIVKPEDNGFVSGERLTPEFPVRNRPLKARDGKAVTQLAYARAGIVTPEMEFIAIRENLGREKLKEKLV
RDGESFGAAVPDYVTPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLTKINANIGNSAVTSSMAEEVEKLVWATRWGA
DTVMDLSTGRNIHNIREWIIRNSPVPIGTVPIYQALEKVGGIAEELTWEVFRDTLIEQAEQGVDYFTIHAGVRLHYVPLT
VNRVTGIVSRGGSIMAKWCLAHHKESFLYEHFAEICDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAW
AKDCQVMIEGPGHVPMHKIKENMDKQLEICGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
PDRDDVKTGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARALHDETLPKEAHKVAHFCSMC
GPKFCSMRISHDIRAEAQKEGMAAMAEKYREGGDLYVAVEETAETQGA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=605, Percent_Identity=68.9256198347107, Blast_Score=856, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 67259; Mature: 67259

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARNNPKPEITTGPLPASSKVYKPGTLHSEIRVPMREIAVHPTAGEPPVTVYDSSGPYT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHEECCCCCCCCEEEECCCCCCC
DTSVTIDIEKGLPRLRETWIEARGDVERYDGRIVKPEDNGFVSGERLTPEFPVRNRPLKA
CCEEEEEHHHCCHHHHHHHHHHCCCHHHHCCEEECCCCCCEECCCCCCCCCCCCCCCCCC
RDGKAVTQLAYARAGIVTPEMEFIAIRENLGREKLKEKLVRDGESFGAAVPDYVTPEFVR
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHH
DEVARGRAIIPANINHPESEPMIIGRNFLTKINANIGNSAVTSSMAEEVEKLVWATRWGA
HHHHCCCEEEECCCCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DTVMDLSTGRNIHNIREWIIRNSPVPIGTVPIYQALEKVGGIAEELTWEVFRDTLIEQAE
CCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QGVDYFTIHAGVRLHYVPLTVNRVTGIVSRGGSIMAKWCLAHHKESFLYEHFAEICDICR
CCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKDCQVMIEGPGHVPMHKIK
HHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
ENMDKQLEICGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
HHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCC
PDRDDVKTGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARA
CCCCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCEEEEEECCCCCEEECHHHHHH
LHDETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGMAAMAEKYREGGDLYVAVE
HHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ETAETQGA
CCCCCCCC
>Mature Secondary Structure
MDARNNPKPEITTGPLPASSKVYKPGTLHSEIRVPMREIAVHPTAGEPPVTVYDSSGPYT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHEECCCCCCCCEEEECCCCCCC
DTSVTIDIEKGLPRLRETWIEARGDVERYDGRIVKPEDNGFVSGERLTPEFPVRNRPLKA
CCEEEEEHHHCCHHHHHHHHHHCCCHHHHCCEEECCCCCCEECCCCCCCCCCCCCCCCCC
RDGKAVTQLAYARAGIVTPEMEFIAIRENLGREKLKEKLVRDGESFGAAVPDYVTPEFVR
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHH
DEVARGRAIIPANINHPESEPMIIGRNFLTKINANIGNSAVTSSMAEEVEKLVWATRWGA
HHHHCCCEEEECCCCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DTVMDLSTGRNIHNIREWIIRNSPVPIGTVPIYQALEKVGGIAEELTWEVFRDTLIEQAE
CCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QGVDYFTIHAGVRLHYVPLTVNRVTGIVSRGGSIMAKWCLAHHKESFLYEHFAEICDICR
CCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKDCQVMIEGPGHVPMHKIK
HHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
ENMDKQLEICGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
HHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCC
PDRDDVKTGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARA
CCCCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCEEEEEECCCCCEEECHHHHHH
LHDETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGMAAMAEKYREGGDLYVAVE
HHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ETAETQGA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968; 12003951 [H]