Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 110634883

GI number: 110634883

Start: 2723619

End: 2724521

Strand: Reverse

Name: opuAB [H]

Synonym: Meso_2542

Alternate gene names: 110634883

Gene position: 2724521-2723619 (Counterclockwise)

Preceding gene: 110634884

Following gene: 110634882

Centisome position: 61.75

GC content: 60.58

Gene sequence:

>903_bases
TTGGGCGCACCGCACTTCCGTGCGGTGCGCTTTCCCAAAGCCCTGAAGGAGCGTGCGGTGTTTCCCAAGAGTCTCGAATT
CTCGATAAGCCAACCGATAAACGATTTCGTCGCCTTCCTGATCGAGAGCTATGGCGAGACCTTTCGCGCCATTTCGAACT
TCATCCTGAGCGGGCTGTTGCAGATCGAGTGGGTTCTCAGGGCACTGCCCTGGTGGGGTACAATCCTCATATTCATGGCG
GTGGCTTACCTGTGCGTGCGCAACTACAAGCTGCCGCTTCTGGTCGGGGGCCTGTTGATGCTCGCCGGCATGCTGGGGCT
GTGGGATCTCACCATGCAGACGCTGGCGCTCATGCTGATGGCGACGCTGATCTCGTGTCTCGTCGGCATACCTACCGGCG
TTCTGGTCGCGAAGGTTCGCGTCATGCGCAACATCCTGATCCCGATGCTCGACATCATGCAGACGATGCCGTCCTTCGTT
TACCTGATCCCGGCGATCATGCTCTTCGGTCTGGGAAAGGTGCCGGCGGTCCTGGCCACCATCATTTACGGCGTGCCGCC
GCTAATCCGCCTGACCTCCCTTGGCATCCGGCAGGTCGACCAGGAGGTGGTTGAGGCCGCGTTGGCGTTCGGCAGCAGCT
CGCGCCAGATTCTCTTCGGCGTGGAGCTCCCCCTTGCAGTGCCCACGATCCTCGCGGGCCTGAATCAGACCATCATGATG
TCGCTTTCGATGGTGGTCGTGTCTTCGATGATCGGCGCCCGCGGCCTAGGTCAGCAGGTTCTTGAAGGAATTCAGACGCT
GGACGTCGGCAAGGGCCTCGAGGCAGGCGTCGGCATCGTAATCCTCGCAGTGGTTCTTGACCGGATAACGCAAGGCCTCG
GCCAATCGCAACGCAGGGACTAG

Upstream 100 bases:

>100_bases
TGCAGCTACGTGGTTCCTGAAGAACTACGCGCATCTCTGGACCAATTGGGTCAGCACCGAAGCGGCAGAAAAAATCAACC
AGGGACTGAACTAAATCCGG

Downstream 100 bases:

>100_bases
GACCATGGCTGCCATCGAACTTCAGAACGTTTACAAGATTTTCGGCCGGCGCAACAAAGAGGCGCTGAAAATGGTGCAGG
CCGGCGTGAGCAAGGCCGAC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MGAPHFRAVRFPKALKERAVFPKSLEFSISQPINDFVAFLIESYGETFRAISNFILSGLLQIEWVLRALPWWGTILIFMA
VAYLCVRNYKLPLLVGGLLMLAGMLGLWDLTMQTLALMLMATLISCLVGIPTGVLVAKVRVMRNILIPMLDIMQTMPSFV
YLIPAIMLFGLGKVPAVLATIIYGVPPLIRLTSLGIRQVDQEVVEAALAFGSSSRQILFGVELPLAVPTILAGLNQTIMM
SLSMVVVSSMIGARGLGQQVLEGIQTLDVGKGLEAGVGIVILAVVLDRITQGLGQSQRRD

Sequences:

>Translated_300_residues
MGAPHFRAVRFPKALKERAVFPKSLEFSISQPINDFVAFLIESYGETFRAISNFILSGLLQIEWVLRALPWWGTILIFMA
VAYLCVRNYKLPLLVGGLLMLAGMLGLWDLTMQTLALMLMATLISCLVGIPTGVLVAKVRVMRNILIPMLDIMQTMPSFV
YLIPAIMLFGLGKVPAVLATIIYGVPPLIRLTSLGIRQVDQEVVEAALAFGSSSRQILFGVELPLAVPTILAGLNQTIMM
SLSMVVVSSMIGARGLGQQVLEGIQTLDVGKGLEAGVGIVILAVVLDRITQGLGQSQRRD
>Mature_299_residues
GAPHFRAVRFPKALKERAVFPKSLEFSISQPINDFVAFLIESYGETFRAISNFILSGLLQIEWVLRALPWWGTILIFMAV
AYLCVRNYKLPLLVGGLLMLAGMLGLWDLTMQTLALMLMATLISCLVGIPTGVLVAKVRVMRNILIPMLDIMQTMPSFVY
LIPAIMLFGLGKVPAVLATIIYGVPPLIRLTSLGIRQVDQEVVEAALAFGSSSRQILFGVELPLAVPTILAGLNQTIMMS
LSMVVVSSMIGARGLGQQVLEGIQTLDVGKGLEAGVGIVILAVVLDRITQGLGQSQRRD

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=265, Percent_Identity=46.0377358490566, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=29.2307692307692, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=28.042328042328, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786564, Length=166, Percent_Identity=31.3253012048193, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32595; Mature: 32464

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAPHFRAVRFPKALKERAVFPKSLEFSISQPINDFVAFLIESYGETFRAISNFILSGLL
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIEWVLRALPWWGTILIFMAVAYLCVRNYKLPLLVGGLLMLAGMLGLWDLTMQTLALMLM
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLISCLVGIPTGVLVAKVRVMRNILIPMLDIMQTMPSFVYLIPAIMLFGLGKVPAVLAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IIYGVPPLIRLTSLGIRQVDQEVVEAALAFGSSSRQILFGVELPLAVPTILAGLNQTIMM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCHHHHH
SLSMVVVSSMIGARGLGQQVLEGIQTLDVGKGLEAGVGIVILAVVLDRITQGLGQSQRRD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GAPHFRAVRFPKALKERAVFPKSLEFSISQPINDFVAFLIESYGETFRAISNFILSGLL
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIEWVLRALPWWGTILIFMAVAYLCVRNYKLPLLVGGLLMLAGMLGLWDLTMQTLALMLM
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLISCLVGIPTGVLVAKVRVMRNILIPMLDIMQTMPSFVYLIPAIMLFGLGKVPAVLAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IIYGVPPLIRLTSLGIRQVDQEVVEAALAFGSSSRQILFGVELPLAVPTILAGLNQTIMM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCHHHHH
SLSMVVVSSMIGARGLGQQVLEGIQTLDVGKGLEAGVGIVILAVVLDRITQGLGQSQRRD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]