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The map label for this gene is lutR [H]

Identifier: 110634861

GI number: 110634861

Start: 2698562

End: 2699278

Strand: Reverse

Name: lutR [H]

Synonym: Meso_2520

Alternate gene names: 110634861

Gene position: 2699278-2698562 (Counterclockwise)

Preceding gene: 110634862

Following gene: 110634860

Centisome position: 61.17

GC content: 60.67

Gene sequence:

>717_bases
ATGCAAGGCGCCACCCGAAAATCTGCGAATGGTTACGAACGCGTGTTCGGATTCCTGCGGGAGCAATTGCTCAGTGGCGC
GCTCAAGGAAGGCGACCTGCTTCTGCCAGAACGGGAGCTGTGTCAGGCTCTGGAAGTAAGCCGACCAGTGCTGCGCGAAG
CGTTGCGCGCGCTCTCTATCTTCGGTGTCATCGAGATTCGACACGGCGTCGGCTCGGTCGTGTCGAGGCCGAATATCGAC
ATCCTAGGCGACTTCTTCAGCTTCGCCCTTGCGAATAAGGGCAATGCAGTGGACGAAGTGATGGAGGCCCGGATAGCCGT
CGAGTGCCAAGCGGCACGTCTCGCGGTTTCGCGTGCGGGTATCGGTGATTTCGAGAGAATGCGCGGGTTCGCTGATGCGA
TCGCCAAAACGATCGATGATCCCGTTGTCGGGGCCCGAGCCGATTACGAATTTCACATGGCCCTCGTCGATGCCAGCGCC
GCTACCGCGCTGCGCAGCATGTATATCGCCATCGCCGATCTTCTTCAGCGTTCGCATGTGGAGCGCCGGGTGTTGGTCGC
AGCCGATGCGAGCACACGCCAGCAGATCATCGACGACCACTACCGCATCCTCACCACCCTGACAGAAGGCAATCCGGACG
AAGCCGAGCGCGCGGTCAGAAACCACTTCGAGATCGGGCGGGCGATCAGGATGAGGATGTCCGTCCACCGAGCCTGA

Upstream 100 bases:

>100_bases
GCTCGAAAGCGGGGGCTACGCTCCCGCCTTACCTGCGCTTGATGTTCGCGGCCGGGTCATGCTAGAGAGGTAATACCACT
AACGATCTCACTGAAGTTTT

Downstream 100 bases:

>100_bases
GACAGGCAAATTTACAACCCAGCCGGGAGGAAAGTATGAGACTTTTTACCATAACTGCTTGCGGTATTCTGGCCGCGCTC
ACCATGACGGGATCGGCCCT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MQGATRKSANGYERVFGFLREQLLSGALKEGDLLLPERELCQALEVSRPVLREALRALSIFGVIEIRHGVGSVVSRPNID
ILGDFFSFALANKGNAVDEVMEARIAVECQAARLAVSRAGIGDFERMRGFADAIAKTIDDPVVGARADYEFHMALVDASA
ATALRSMYIAIADLLQRSHVERRVLVAADASTRQQIIDDHYRILTTLTEGNPDEAERAVRNHFEIGRAIRMRMSVHRA

Sequences:

>Translated_238_residues
MQGATRKSANGYERVFGFLREQLLSGALKEGDLLLPERELCQALEVSRPVLREALRALSIFGVIEIRHGVGSVVSRPNID
ILGDFFSFALANKGNAVDEVMEARIAVECQAARLAVSRAGIGDFERMRGFADAIAKTIDDPVVGARADYEFHMALVDASA
ATALRSMYIAIADLLQRSHVERRVLVAADASTRQQIIDDHYRILTTLTEGNPDEAERAVRNHFEIGRAIRMRMSVHRA
>Mature_238_residues
MQGATRKSANGYERVFGFLREQLLSGALKEGDLLLPERELCQALEVSRPVLREALRALSIFGVIEIRHGVGSVVSRPNID
ILGDFFSFALANKGNAVDEVMEARIAVECQAARLAVSRAGIGDFERMRGFADAIAKTIDDPVVGARADYEFHMALVDASA
ATALRSMYIAIADLLQRSHVERRVLVAADASTRQQIIDDHYRILTTLTEGNPDEAERAVRNHFEIGRAIRMRMSVHRA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=236, Percent_Identity=25.4237288135593, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1789621, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1786303, Length=219, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790032, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789353, Length=205, Percent_Identity=27.8048780487805, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48994955, Length=213, Percent_Identity=26.7605633802817, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082208, Length=217, Percent_Identity=26.7281105990783, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26290; Mature: 26290

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGATRKSANGYERVFGFLREQLLSGALKEGDLLLPERELCQALEVSRPVLREALRALSI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
FGVIEIRHGVGSVVSRPNIDILGDFFSFALANKGNAVDEVMEARIAVECQAARLAVSRAG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IGDFERMRGFADAIAKTIDDPVVGARADYEFHMALVDASAATALRSMYIAIADLLQRSHV
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRVLVAADASTRQQIIDDHYRILTTLTEGNPDEAERAVRNHFEIGRAIRMRMSVHRA
HHHEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGATRKSANGYERVFGFLREQLLSGALKEGDLLLPERELCQALEVSRPVLREALRALSI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
FGVIEIRHGVGSVVSRPNIDILGDFFSFALANKGNAVDEVMEARIAVECQAARLAVSRAG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IGDFERMRGFADAIAKTIDDPVVGARADYEFHMALVDASAATALRSMYIAIADLLQRSHV
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRVLVAADASTRQQIIDDHYRILTTLTEGNPDEAERAVRNHFEIGRAIRMRMSVHRA
HHHEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]