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The map label for this gene is gcvA [H]

Identifier: 110634823

GI number: 110634823

Start: 2660204

End: 2661115

Strand: Direct

Name: gcvA [H]

Synonym: Meso_2482

Alternate gene names: 110634823

Gene position: 2660204-2661115 (Clockwise)

Preceding gene: 110634819

Following gene: 110634824

Centisome position: 60.29

GC content: 64.04

Gene sequence:

>912_bases
ATGACCCGCCCGCGCCGCTTTCTTCCTTCTATCAGCCTCCTCTCGGCCTTTGAGGCCGTGCTTCGGACGGGATCGACCGC
CGCCGCGGCGCGCGAGCTCGACCTGACGCAGGGCGCCGTCAGCAGGCTTTTGCAGAACCTGGAGGAGCAGCTTGGGCAAC
AGCTCTTCATCCGCCATCGCCGCCGCCTCATCGTAACAGAAGCCGCTCGATCTTATGGACGCGATATCACGCGAGCGCTG
GACCTGATCCAGCGCGCCAGCATAGAGCTTACGACCAATCCGGGTGGCGGTGTGCTTTCGCTTGCCATCCTGCCTGCCTT
CGGCACACGCTGGCTGGCGCCGCGCCTCGGCTCCTTTCTCGGTGCGCATCCAGGCATCACCATCAATCTCGCAACACGGC
TGAAGCGCTTCAATTTCTCCGCCGAAGGCTTTGATGCCGCAATCCATTTCGGGTCGGACGACTGGCGCGATGCAGAGCAC
ATGAAACTGTTCGAAGAACAGCTTACCGCCTGCGCGGCCCCTTCTCTTCTGGCCAAGGTCCCTGTTGAAACCGCAGAGGA
TCTGAGGCGCCTGCAACTCTTTCAGATGGAGTCGCGGCGAAATGCCTGGTCCGTTTGGTTCACGGCTCAGGGCGCCGAGG
CCACACCGAGCCGCGGCATGCTATTCGACCAATATGCCCCCATGACACAGGCGGCTGTGGCAGGCCTCGGCGTGGCCCTT
CTGCCCGAATACCTCGCGGAGCCGGAAATCGCCGACGGTCGCCTAGTGCCGCTTCTCAAACGCGCCGTGCGGGGCGACGG
CTCCTATTGGCTGGTCTGGCCCGAAGCCCGCGCCAGCTACCCTCCCCTGGAAGCGTTTCGAACCTGGCTTGCGGGGGAAA
CGGCGCCTCTGCGGGAACGCACGGAAGGCTGA

Upstream 100 bases:

>100_bases
TTAGATCGCGTGAAGGATCACCTGCAGATGCGTTTACCTGTAGCATACGGGCCGGAAAAATCCTATTTGAGCGTAAGTTG
ATGCATAAAACTCATATGTG

Downstream 100 bases:

>100_bases
CTGAGGCCCGCCGCGCTTCTGGCCGGCGGATTAACTTCCCCGCTACCCGTACCGGAACGTAGTCGATTGGCGCTCGTTAG
TTCGGACGGCCCCAGGAGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTRPRRFLPSISLLSAFEAVLRTGSTAAAARELDLTQGAVSRLLQNLEEQLGQQLFIRHRRRLIVTEAARSYGRDITRAL
DLIQRASIELTTNPGGGVLSLAILPAFGTRWLAPRLGSFLGAHPGITINLATRLKRFNFSAEGFDAAIHFGSDDWRDAEH
MKLFEEQLTACAAPSLLAKVPVETAEDLRRLQLFQMESRRNAWSVWFTAQGAEATPSRGMLFDQYAPMTQAAVAGLGVAL
LPEYLAEPEIADGRLVPLLKRAVRGDGSYWLVWPEARASYPPLEAFRTWLAGETAPLRERTEG

Sequences:

>Translated_303_residues
MTRPRRFLPSISLLSAFEAVLRTGSTAAAARELDLTQGAVSRLLQNLEEQLGQQLFIRHRRRLIVTEAARSYGRDITRAL
DLIQRASIELTTNPGGGVLSLAILPAFGTRWLAPRLGSFLGAHPGITINLATRLKRFNFSAEGFDAAIHFGSDDWRDAEH
MKLFEEQLTACAAPSLLAKVPVETAEDLRRLQLFQMESRRNAWSVWFTAQGAEATPSRGMLFDQYAPMTQAAVAGLGVAL
LPEYLAEPEIADGRLVPLLKRAVRGDGSYWLVWPEARASYPPLEAFRTWLAGETAPLRERTEG
>Mature_302_residues
TRPRRFLPSISLLSAFEAVLRTGSTAAAARELDLTQGAVSRLLQNLEEQLGQQLFIRHRRRLIVTEAARSYGRDITRALD
LIQRASIELTTNPGGGVLSLAILPAFGTRWLAPRLGSFLGAHPGITINLATRLKRFNFSAEGFDAAIHFGSDDWRDAEHM
KLFEEQLTACAAPSLLAKVPVETAEDLRRLQLFQMESRRNAWSVWFTAQGAEATPSRGMLFDQYAPMTQAAVAGLGVALL
PEYLAEPEIADGRLVPLLKRAVRGDGSYWLVWPEARASYPPLEAFRTWLAGETAPLRERTEG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=33.8983050847458, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=34.6153846153846, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=28.9655172413793, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=27.4576271186441, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=26.4604810996564, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789639, Length=253, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787589, Length=275, Percent_Identity=24.3636363636364, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33555; Mature: 33424

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPRRFLPSISLLSAFEAVLRTGSTAAAARELDLTQGAVSRLLQNLEEQLGQQLFIRHR
CCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLIVTEAARSYGRDITRALDLIQRASIELTTNPGGGVLSLAILPAFGTRWLAPRLGSFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHCHHHHHHHHHHHH
GAHPGITINLATRLKRFNFSAEGFDAAIHFGSDDWRDAEHMKLFEEQLTACAAPSLLAKV
CCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHHHC
PVETAEDLRRLQLFQMESRRNAWSVWFTAQGAEATPSRGMLFDQYAPMTQAAVAGLGVAL
CHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEHHHCCCHHHHHHHHHHHHH
LPEYLAEPEIADGRLVPLLKRAVRGDGSYWLVWPEARASYPPLEAFRTWLAGETAPLRER
HHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHC
TEG
CCC
>Mature Secondary Structure 
TRPRRFLPSISLLSAFEAVLRTGSTAAAARELDLTQGAVSRLLQNLEEQLGQQLFIRHR
CCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLIVTEAARSYGRDITRALDLIQRASIELTTNPGGGVLSLAILPAFGTRWLAPRLGSFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHCHHHHHHHHHHHH
GAHPGITINLATRLKRFNFSAEGFDAAIHFGSDDWRDAEHMKLFEEQLTACAAPSLLAKV
CCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHHHC
PVETAEDLRRLQLFQMESRRNAWSVWFTAQGAEATPSRGMLFDQYAPMTQAAVAGLGVAL
CHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEHHHCCCHHHHHHHHHHHHH
LPEYLAEPEIADGRLVPLLKRAVRGDGSYWLVWPEARASYPPLEAFRTWLAGETAPLRER
HHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHC
TEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]