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The map label for this gene is gatA [H]

Identifier: 110634721

GI number: 110634721

Start: 2533919

End: 2535361

Strand: Direct

Name: gatA [H]

Synonym: Meso_2379

Alternate gene names: 110634721

Gene position: 2533919-2535361 (Clockwise)

Preceding gene: 110634720

Following gene: 110634723

Centisome position: 57.43

GC content: 64.52

Gene sequence:

>1443_bases
ATGCAAGGCTGGCGGGAGGAACTGGCGGCGCCCCGCAGTCTGCCTCCCTGTCCGGGAGATGAAGAGGATATAGAATTCGC
CCTCCCCGCTCCGGATGAAGACACGATCGTTGCGGCGGCTGCGGCATATGCCGCACGCGATCTTTCTCCGGTCGAGCTGG
TCAAGCACATGCTGCTGAGGATTGAGAGACTGGACGGGACGCTCCACAGCTATATCACCGTGCTGCCTGAAGCCGCCCTG
GCGCAGGCCCGTGAGGCCGAAGTGCGAATGATGCGGAGGCAGCCGCGCGGCCCGCTCGACGGCATCCCCTATAACCTCAA
GGATATAATCGACACGGCCGGGATCCGCACGACCTGCCACTCTCGTCTTAGGACCGATGTCGTTCCCGTCCGGGACGCCG
ACGCCGCCGCACGTCTGAAGTCAGCGGGAGGCGTTTTATTGGGCAAGAGCGCGACGCATGAATTCGCCTTCGGCGGGCCG
ACCGACGACACACCCTTCCCGCAACCACGAAACCCCTGGGATACTGAACGCTATGCCGGCGGCTCGTCCAGCGGCGCGGC
CGTCGCCGTCGCAGCGAGGATGGCGCTTGGTTCCGTCGGAACCGATACCGGCGGCTCGTTGCGCATCCCAAGCTCCTTTT
GCGGGGTAGTCTCGATGAAGCCCAGTCGCGGGCGCATCAACACGGAAGGGGTTTTTCCGCTCGCCCCCTCGCTCGATCAC
GTCGGCCCTGTCGCGGCGAATGCAGCGGACTGCGCCGTTCTCTTTGAAGCGCTCGCCGTCGACGGGCTTGTCGAACCGCG
GATGCCTGCTGACGGTCTGAAAGGCTTGACGGCCGGTCTGCCGCGGGACTGGATCGACAACCAGGCCGAGCTCTCACCGG
CGGTGCGCCGGGCGTTGCGACAAATGGAGGACTTGGCCCGCGATCTTGGCGCGACAACAGTTGATCTTGCGCCACCTCCC
CTGTCCGAATTTCGTGCCGCCGTGTCCCTGCTTACCGCGTGGGAGGCATATCGCATCCACGCGGTTGACCTCGGACGCCA
GTTCCTTCGCTATTCGGATGTCTTCCGCCGGCGTGTCGCCCCCGCCGCGCTGCTGGACGAAGCCGACCACGCTTCCATGA
TTGCGGCCAAAACCCGTCTTTCGGCCGCGTTTAATGCCCTCTTTGAACAATGCGACTTCATTTTGATGCCTGCCACGGCC
ACCACGGCCCCTCTGCTGACCGGGCTCGACCCGCTGGCCGAAGCTGCACGCCCATCTTCCTTCTCTCAGCCGACCAATCT
GACAGGCAGCCCCTCCCTGACCATCCGCGCCGGGTTCGACCGGAAAGGACTGCCGATCGGTCTCCAGCTTGTGGGGCCGA
TGCGGGCGGAGGAGCTGTTATTTGCCATCGCTTGCAGGATAGAGGATGCGATGCCGCGCCGCCTTTACCGGTCAGGCGAA
TAG

Upstream 100 bases:

>100_bases
AGAGGCTGGCTTCATGACCTCGGACGCTGCCCGCCTGAGGCAGCTCTTCGGCCCACTTCCCGATGAAGAGATCATCCAGA
TCACTCGACGCCGGCAGATT

Downstream 100 bases:

>100_bases
CCAAGGGCGGAAGAGATCTGAAGCGCACTATCCACAACGAGCTCTGCCGCCTTCTCGGTGGACATTTCAGGCGCGGACCA
AGGGGTATAGGCCACGCTGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MQGWREELAAPRSLPPCPGDEEDIEFALPAPDEDTIVAAAAAYAARDLSPVELVKHMLLRIERLDGTLHSYITVLPEAAL
AQAREAEVRMMRRQPRGPLDGIPYNLKDIIDTAGIRTTCHSRLRTDVVPVRDADAAARLKSAGGVLLGKSATHEFAFGGP
TDDTPFPQPRNPWDTERYAGGSSSGAAVAVAARMALGSVGTDTGGSLRIPSSFCGVVSMKPSRGRINTEGVFPLAPSLDH
VGPVAANAADCAVLFEALAVDGLVEPRMPADGLKGLTAGLPRDWIDNQAELSPAVRRALRQMEDLARDLGATTVDLAPPP
LSEFRAAVSLLTAWEAYRIHAVDLGRQFLRYSDVFRRRVAPAALLDEADHASMIAAKTRLSAAFNALFEQCDFILMPATA
TTAPLLTGLDPLAEAARPSSFSQPTNLTGSPSLTIRAGFDRKGLPIGLQLVGPMRAEELLFAIACRIEDAMPRRLYRSGE

Sequences:

>Translated_480_residues
MQGWREELAAPRSLPPCPGDEEDIEFALPAPDEDTIVAAAAAYAARDLSPVELVKHMLLRIERLDGTLHSYITVLPEAAL
AQAREAEVRMMRRQPRGPLDGIPYNLKDIIDTAGIRTTCHSRLRTDVVPVRDADAAARLKSAGGVLLGKSATHEFAFGGP
TDDTPFPQPRNPWDTERYAGGSSSGAAVAVAARMALGSVGTDTGGSLRIPSSFCGVVSMKPSRGRINTEGVFPLAPSLDH
VGPVAANAADCAVLFEALAVDGLVEPRMPADGLKGLTAGLPRDWIDNQAELSPAVRRALRQMEDLARDLGATTVDLAPPP
LSEFRAAVSLLTAWEAYRIHAVDLGRQFLRYSDVFRRRVAPAALLDEADHASMIAAKTRLSAAFNALFEQCDFILMPATA
TTAPLLTGLDPLAEAARPSSFSQPTNLTGSPSLTIRAGFDRKGLPIGLQLVGPMRAEELLFAIACRIEDAMPRRLYRSGE
>Mature_480_residues
MQGWREELAAPRSLPPCPGDEEDIEFALPAPDEDTIVAAAAAYAARDLSPVELVKHMLLRIERLDGTLHSYITVLPEAAL
AQAREAEVRMMRRQPRGPLDGIPYNLKDIIDTAGIRTTCHSRLRTDVVPVRDADAAARLKSAGGVLLGKSATHEFAFGGP
TDDTPFPQPRNPWDTERYAGGSSSGAAVAVAARMALGSVGTDTGGSLRIPSSFCGVVSMKPSRGRINTEGVFPLAPSLDH
VGPVAANAADCAVLFEALAVDGLVEPRMPADGLKGLTAGLPRDWIDNQAELSPAVRRALRQMEDLARDLGATTVDLAPPP
LSEFRAAVSLLTAWEAYRIHAVDLGRQFLRYSDVFRRRVAPAALLDEADHASMIAAKTRLSAAFNALFEQCDFILMPATA
TTAPLLTGLDPLAEAARPSSFSQPTNLTGSPSLTIRAGFDRKGLPIGLQLVGPMRAEELLFAIACRIEDAMPRRLYRSGE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=491, Percent_Identity=28.5132382892057, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI195972892, Length=482, Percent_Identity=22.4066390041494, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=26.6075388026608, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=23.6252545824847, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=201, Percent_Identity=26.865671641791, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=395, Percent_Identity=27.3417721518987, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=24.8803827751196, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=30.4526748971193, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24644968, Length=474, Percent_Identity=23.2067510548523, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078093, Length=488, Percent_Identity=25.2049180327869, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=23.9754098360656, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=23.9754098360656, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=23.9754098360656, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=23.9754098360656, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=479, Percent_Identity=24.6346555323591, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648435, Length=479, Percent_Identity=24.0083507306889, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648437, Length=479, Percent_Identity=24.0083507306889, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45550774, Length=504, Percent_Identity=24.2063492063492, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=409, Percent_Identity=24.6943765281174, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648439, Length=409, Percent_Identity=24.6943765281174, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51383; Mature: 51383

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGWREELAAPRSLPPCPGDEEDIEFALPAPDEDTIVAAAAAYAARDLSPVELVKHMLLR
CCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IERLDGTLHSYITVLPEAALAQAREAEVRMMRRQPRGPLDGIPYNLKDIIDTAGIRTTCH
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHH
SRLRTDVVPVRDADAAARLKSAGGVLLGKSATHEFAFGGPTDDTPFPQPRNPWDTERYAG
HHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
GSSSGAAVAVAARMALGSVGTDTGGSLRIPSSFCGVVSMKPSRGRINTEGVFPLAPSLDH
CCCCCHHHHHHHHHHHCCCCCCCCCCEECCHHHCCEEEECCCCCCCCCCCCCCCCCCCHH
VGPVAANAADCAVLFEALAVDGLVEPRMPADGLKGLTAGLPRDWIDNQAELSPAVRRALR
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHCCCHHHCCHHHHHHHH
QMEDLARDLGATTVDLAPPPLSEFRAAVSLLTAWEAYRIHAVDLGRQFLRYSDVFRRRVA
HHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCC
PAALLDEADHASMIAAKTRLSAAFNALFEQCDFILMPATATTAPLLTGLDPLAEAARPSS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCHHHHHHCCCCC
FSQPTNLTGSPSLTIRAGFDRKGLPIGLQLVGPMRAEELLFAIACRIEDAMPRRLYRSGE
CCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQGWREELAAPRSLPPCPGDEEDIEFALPAPDEDTIVAAAAAYAARDLSPVELVKHMLLR
CCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IERLDGTLHSYITVLPEAALAQAREAEVRMMRRQPRGPLDGIPYNLKDIIDTAGIRTTCH
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHH
SRLRTDVVPVRDADAAARLKSAGGVLLGKSATHEFAFGGPTDDTPFPQPRNPWDTERYAG
HHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
GSSSGAAVAVAARMALGSVGTDTGGSLRIPSSFCGVVSMKPSRGRINTEGVFPLAPSLDH
CCCCCHHHHHHHHHHHCCCCCCCCCCEECCHHHCCEEEECCCCCCCCCCCCCCCCCCCHH
VGPVAANAADCAVLFEALAVDGLVEPRMPADGLKGLTAGLPRDWIDNQAELSPAVRRALR
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHCCCHHHCCHHHHHHHH
QMEDLARDLGATTVDLAPPPLSEFRAAVSLLTAWEAYRIHAVDLGRQFLRYSDVFRRRVA
HHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCC
PAALLDEADHASMIAAKTRLSAAFNALFEQCDFILMPATATTAPLLTGLDPLAEAARPSS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCHHHHHHCCCCC
FSQPTNLTGSPSLTIRAGFDRKGLPIGLQLVGPMRAEELLFAIACRIEDAMPRRLYRSGE
CCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA