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The map label for this gene is greA

Identifier: 110634467

GI number: 110634467

Start: 2275039

End: 2275512

Strand: Reverse

Name: greA

Synonym: Meso_2118

Alternate gene names: 110634467

Gene position: 2275512-2275039 (Counterclockwise)

Preceding gene: 110634472

Following gene: 110634466

Centisome position: 51.57

GC content: 60.55

Gene sequence:

>474_bases
ATGAACAAGGTCCCAATGACCCTGGCTGGATACGAGTCGCTCAAGGAAGAATTGCGTTGGCGCCAACAGGAAGAGCGGCC
GCGCATTATCGAGGCGATCGCTGAGGCTCGTGCGCACGGTGACCTCTCAGAAAATGCCGAATACCACGCGGCCAAAGAGG
CCCAGAGCCTCAATGAAGGCCGCATCAGCGAGCTTGAGGATCTGATCGCGCGCGCCGAAGTCATCGACGTGACAAAGCTT
TCAGGCGATACGGTGAAGTTCGGCGCCACCGTCGTCCTCGTCGACGAGGACACCGAAGAAGAAAAGACCTATCAGATCGT
CGGCGATCAGGAGGCGGACGTGAAGTCGGGCCGCATCTCCATTTCCTCGCCGATCGCCAGGGCGCTGATCGGGAAGGGCG
TTGGCGACTTGATCGAGGTCAACGCGCCGGGTGGCGCGCGTGGCTATGAGGTCTTGCGCGTTCAGTACGGCTGA

Upstream 100 bases:

>100_bases
TGGAGTCGAACGAAACGGTTCCGTGGCCCTGGCTTTCGGACCGTTTTCTTTTTGTGTGTCGCCGGCCCGGCGGGCCTATG
AGGTCAGGAAGGTGTGAGAA

Downstream 100 bases:

>100_bases
GCTGGACGGTCATGGGGGCTCATGCCGGTCCGGCTTCCGCCGCCGGTCACCTTAATATCCACGATGTGGAGGTGGTGGCG
CCAAACCTGAAGCGCCACCT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNKVPMTLAGYESLKEELRWRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSLNEGRISELEDLIARAEVIDVTKL
SGDTVKFGATVVLVDEDTEEEKTYQIVGDQEADVKSGRISISSPIARALIGKGVGDLIEVNAPGGARGYEVLRVQYG

Sequences:

>Translated_157_residues
MNKVPMTLAGYESLKEELRWRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSLNEGRISELEDLIARAEVIDVTKL
SGDTVKFGATVVLVDEDTEEEKTYQIVGDQEADVKSGRISISSPIARALIGKGVGDLIEVNAPGGARGYEVLRVQYG
>Mature_157_residues
MNKVPMTLAGYESLKEELRWRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSLNEGRISELEDLIARAEVIDVTKL
SGDTVKFGATVVLVDEDTEEEKTYQIVGDQEADVKSGRISISSPIARALIGKGVGDLIEVNAPGGARGYEVLRVQYG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=54.140127388535, Blast_Score=168, Evalue=1e-43,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=36.1290322580645, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_MESSB (Q11GG5)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674675.1
- ProteinModelPortal:   Q11GG5
- SMR:   Q11GG5
- STRING:   Q11GG5
- GeneID:   4182606
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_2118
- NMPDR:   fig|266779.1.peg.1702
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   TPGGFVK
- PhylomeDB:   Q11GG5
- ProtClustDB:   PRK00226
- BioCyc:   MSP266779:MESO_2118-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17210; Mature: 17210

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVPMTLAGYESLKEELRWRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSLNEG
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
RISELEDLIARAEVIDVTKLSGDTVKFGATVVLVDEDTEEEKTYQIVGDQEADVKSGRIS
CHHHHHHHHHHHHHHHEEECCCCEEEECEEEEEEECCCCCCCEEEEECCCCCCCCCCEEE
ISSPIARALIGKGVGDLIEVNAPGGARGYEVLRVQYG
ECHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MNKVPMTLAGYESLKEELRWRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSLNEG
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
RISELEDLIARAEVIDVTKLSGDTVKFGATVVLVDEDTEEEKTYQIVGDQEADVKSGRIS
CHHHHHHHHHHHHHHHEEECCCCEEEECEEEEEEECCCCCCCEEEEECCCCCCCCCCEEE
ISSPIARALIGKGVGDLIEVNAPGGARGYEVLRVQYG
ECHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA