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The map label for this gene is emrA [H]

Identifier: 110634270

GI number: 110634270

Start: 2054565

End: 2055716

Strand: Reverse

Name: emrA [H]

Synonym: Meso_1920

Alternate gene names: 110634270

Gene position: 2055716-2054565 (Counterclockwise)

Preceding gene: 110634271

Following gene: 110634269

Centisome position: 46.59

GC content: 62.41

Gene sequence:

>1152_bases
ATGAACGCTATATCCAACCCAGAGGCAAAGGCGGAGCCGGTCGAGGTCGAGCAGCAGAAGGCAGGACCGGTGGCTTTGCC
GCAACCTGCCGATCTCAAGCCGAAGAAGAAAAAGAACCGCCTGCGGATGGTGCTGATGCTCGCGGCACCGCTTGCGCTTG
CCGTTGCGGGAACATGGTGGTGGGTGACCAGCGGGCGATATCAGGAGACGGACGACGCCTATCTGCAGCAGCCCAAGCTT
TCGATCGCCTCCCAGATCGCGGGCCGCGTGGTGGAATCCAATGTCGCGAACAGCATGCACGTGGAAGCCGGCGCGGTGCT
TTTCAAGGTAGACCCGGAGCCCTACCGAATCGCGCTTGCGCAGGCGGAAGCTGCTCTTGAGGGAGCGCGCCTGCAGGTAG
AGCAACTGCGCGCCTCATACAGCCAGGCCGAGGCTCAGGCGCGTGATGCAGACAGCGAAGTCGCCTATCTTGAGACGCAG
CTTAAGCGCCAGCAGGATCTTTCGGGGAAGGGTGTGGCGTCCCAGTCATCGCTGGATCAGGCAGAGCGCAACCTGCAGAA
GGCGAGGGAGAGCCTAAGCGCTACGCGGCAAGCTGTTGCGAGCGCCCTGGCCGCACTTGGCGGCAATCCGAAAATCGAGA
CGGACAAGCATCCGACGGTGCTTTCGGCTCTGGCAGCCCGTGACAAGGCCGCTTATGAGCTGGCTCAGACGACCGTGAGG
GCTCCAGCCGATGGCATCATCGCCCAGGCGTCGGATTTCCGCAATGGCCAGTTCGTCAGCACAGGCACGCCTCTGTTCTC
CCTGGTGGAGACCAGAAGCTCCTGGGTCGAGGCCAATTTCAAGGAGACGCAGCTTACCCACATGAAGCCGGGCCAAAAGG
CCGAGATCGTCTTCGATACGTATCCGGACCGGCCGCTGGAAGCCACCGTCGAGGATATCAGCGCGGGAACGGGGGCGGAG
TTCTCCCTGCTGCCTGCCCAGAACGCTACCGGCAATTGGGTAAAGGTGACGCAACGCATTCCCGTTCGCCTCAAGCTTCA
GATCGAAGAGCCTGAACTTCTGCTGCGCACCGGCATGAGCGCCACCGTGACCGTCGATACCGGGGTTTCACACGGCTTTC
CGGGCCTGACTACGGCGCACGCGACCGAATAG

Upstream 100 bases:

>100_bases
CCGCACTGGTCAGCGCTCTCGAGACAATTGCCGAAAATCTCTCCGACAGCGAGCCGATATGCGGCTCGGTGCAAAAGGCT
GCAGAAGAGGTCGTATCGAA

Downstream 100 bases:

>100_bases
GACCGCTTCACACGAGATTGGAACATCACAATGGCTGAAGCTGCCTCAGCTGCGCACGAGGCTGTCCCGCATCGTGGGCT
GATCACCGTCGCGATCATGC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNAISNPEAKAEPVEVEQQKAGPVALPQPADLKPKKKKNRLRMVLMLAAPLALAVAGTWWWVTSGRYQETDDAYLQQPKL
SIASQIAGRVVESNVANSMHVEAGAVLFKVDPEPYRIALAQAEAALEGARLQVEQLRASYSQAEAQARDADSEVAYLETQ
LKRQQDLSGKGVASQSSLDQAERNLQKARESLSATRQAVASALAALGGNPKIETDKHPTVLSALAARDKAAYELAQTTVR
APADGIIAQASDFRNGQFVSTGTPLFSLVETRSSWVEANFKETQLTHMKPGQKAEIVFDTYPDRPLEATVEDISAGTGAE
FSLLPAQNATGNWVKVTQRIPVRLKLQIEEPELLLRTGMSATVTVDTGVSHGFPGLTTAHATE

Sequences:

>Translated_383_residues
MNAISNPEAKAEPVEVEQQKAGPVALPQPADLKPKKKKNRLRMVLMLAAPLALAVAGTWWWVTSGRYQETDDAYLQQPKL
SIASQIAGRVVESNVANSMHVEAGAVLFKVDPEPYRIALAQAEAALEGARLQVEQLRASYSQAEAQARDADSEVAYLETQ
LKRQQDLSGKGVASQSSLDQAERNLQKARESLSATRQAVASALAALGGNPKIETDKHPTVLSALAARDKAAYELAQTTVR
APADGIIAQASDFRNGQFVSTGTPLFSLVETRSSWVEANFKETQLTHMKPGQKAEIVFDTYPDRPLEATVEDISAGTGAE
FSLLPAQNATGNWVKVTQRIPVRLKLQIEEPELLLRTGMSATVTVDTGVSHGFPGLTTAHATE
>Mature_383_residues
MNAISNPEAKAEPVEVEQQKAGPVALPQPADLKPKKKKNRLRMVLMLAAPLALAVAGTWWWVTSGRYQETDDAYLQQPKL
SIASQIAGRVVESNVANSMHVEAGAVLFKVDPEPYRIALAQAEAALEGARLQVEQLRASYSQAEAQARDADSEVAYLETQ
LKRQQDLSGKGVASQSSLDQAERNLQKARESLSATRQAVASALAALGGNPKIETDKHPTVLSALAARDKAAYELAQTTVR
APADGIIAQASDFRNGQFVSTGTPLFSLVETRSSWVEANFKETQLTHMKPGQKAEIVFDTYPDRPLEATVEDISAGTGAE
FSLLPAQNATGNWVKVTQRIPVRLKLQIEEPELLLRTGMSATVTVDTGVSHGFPGLTTAHATE

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=342, Percent_Identity=34.5029239766082, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789041, Length=348, Percent_Identity=33.0459770114943, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790519, Length=324, Percent_Identity=32.0987654320988, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081951, Length=317, Percent_Identity=27.7602523659306, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789637, Length=335, Percent_Identity=25.9701492537313, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790024, Length=244, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787013, Length=336, Percent_Identity=26.7857142857143, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790012, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41289; Mature: 41289

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAISNPEAKAEPVEVEQQKAGPVALPQPADLKPKKKKNRLRMVLMLAAPLALAVAGTWW
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEE
WVTSGRYQETDDAYLQQPKLSIASQIAGRVVESNVANSMHVEAGAVLFKVDPEPYRIALA
EEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCEEEEEH
QAEAALEGARLQVEQLRASYSQAEAQARDADSEVAYLETQLKRQQDLSGKGVASQSSLDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AERNLQKARESLSATRQAVASALAALGGNPKIETDKHPTVLSALAARDKAAYELAQTTVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
APADGIIAQASDFRNGQFVSTGTPLFSLVETRSSWVEANFKETQLTHMKPGQKAEIVFDT
CCCCCCEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEC
YPDRPLEATVEDISAGTGAEFSLLPAQNATGNWVKVTQRIPVRLKLQIEEPELLLRTGMS
CCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEECCEEEEEEECCCHHHHHCCCC
ATVTVDTGVSHGFPGLTTAHATE
EEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAISNPEAKAEPVEVEQQKAGPVALPQPADLKPKKKKNRLRMVLMLAAPLALAVAGTWW
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEE
WVTSGRYQETDDAYLQQPKLSIASQIAGRVVESNVANSMHVEAGAVLFKVDPEPYRIALA
EEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCEEEEEH
QAEAALEGARLQVEQLRASYSQAEAQARDADSEVAYLETQLKRQQDLSGKGVASQSSLDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AERNLQKARESLSATRQAVASALAALGGNPKIETDKHPTVLSALAARDKAAYELAQTTVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
APADGIIAQASDFRNGQFVSTGTPLFSLVETRSSWVEANFKETQLTHMKPGQKAEIVFDT
CCCCCCEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEC
YPDRPLEATVEDISAGTGAEFSLLPAQNATGNWVKVTQRIPVRLKLQIEEPELLLRTGMS
CCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEECCEEEEEEECCCHHHHHCCCC
ATVTVDTGVSHGFPGLTTAHATE
EEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]