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The map label for this gene is emrB [H]

Identifier: 110634269

GI number: 110634269

Start: 2052993

End: 2054534

Strand: Reverse

Name: emrB [H]

Synonym: Meso_1919

Alternate gene names: 110634269

Gene position: 2054534-2052993 (Counterclockwise)

Preceding gene: 110634270

Following gene: 110634265

Centisome position: 46.56

GC content: 60.77

Gene sequence:

>1542_bases
ATGGCTGAAGCTGCCTCAGCTGCGCACGAGGCTGTCCCGCATCGTGGGCTGATCACCGTCGCGATCATGCTCGGCACCAT
CATGCAGGTGCTGGATACGACGATCGCCAATGTGGCGCTGCCGTCGATGACGGGCGATCTTGGTGCTTCGCCGGACACGA
TCACGTGGGTGCTCACATCCTATATCGTGGCCGCCGCCATCATGACGCCGGTCACGGGATGGATTTCCGATCGCATCGGC
CGCCGTGAGTTCTTCCTGGTCTCGATTGCCGGTTTCACCATTTTCTCAATGTTTTGCGGCGTTGCCTGGAACCTGGAGGT
GATGGTTCTCTTCCGGCTTTTGCAAGGGGTGTTCGGCGCCGCGATCGTGCCCCTTGCACAAACCTTTCTCCTCGATATCA
ACCCCAAGGAGAACCACGGACAGGCAATGGCCGTTTTCGGCGCGGGCATTATGGTGGGCCCCATCATCGGTCCTACGCTG
GGCGGCTGGTTAACCGAGACGCTTAACTGGCGCTGGGTGTTCTTCATCAATCTGCCGGTCGGCATTCTCTGCCTGCTGGG
CGCTGCGGCTTACCTTCCACGCGCGGCCCGCCGCCTGCGGGGGTTCGATTTCTTCGGGTTCGCCATGCTATCGCTCGGCG
TCGGCGCGCTGCAGCTCATGCTTGATCGCGGCGGCGAGGTCGATTGGTTCGCCTCGCCCGAGATCTGGATAGAACTGGGG
CTGTCGATCACCGGCTTCTGGCTCTTTGCCGTTCACACGCTGACGGGCGAGAATACGTTCATCGACCGCCACATCTTCCG
CGACCGCAATCTCGTGACGGGGCTCGTGTTCATCTTCGTGGTGGGCATCATCCTGCTCGCGAGCCTTGCGCTTTTGCCGC
CCATGCTCTCGCGCATCTTCGGCTACACGACGATGCTTACGGGGCTTATCATGGCGCCGCGCGGCATTGGCACGATGATT
TCGATGATCATGGTCGGCCGGCTGGTGCGGGTAATCGACGCGCGAATCCTGGTGGCTGCGGGCCTGTCGCTGACGGCCTG
GTCGCTCTACCTCATGACCGGCTTCTCGCCGCAGATGGATGGACGCCTGATCATCGAATCCGGCATCATCCAGGGCATTG
GGCTCGGGCTTGTTTTCGTGCCGCTATCGACGATCACATTCGCCACCCTCGACCCGAAATACCGTACCGATGGCGCCAGC
CTTTTCAGCCTGACCCGCAATATCGGCTCCAGCATCGGCATCTCGGTCGTCACGGTGCTGCTTGCGAACAGCACACAGCT
CAACCATGCGGAGCTTGCCACGCACATTACGCCTTACAACGCGAACCTATGGACGGTTATGCCAGGCGCAGCCCAGGGCG
ATCCGACCTCGCTGTCGATTGTCGACCAGCTTGTAACCCAGCAGGCGCTGATGATCTCCTATATCAACGATTTCAAGTTC
ATGATGATCATAACGCTCTGCGCCATCCCTCTGGTTCTTCTACTCCGGGTGCCGAAGCGCAAAAGCGGCAGCGGAGAGGC
GGCGGTGGCGGCGATGGAATGA

Upstream 100 bases:

>100_bases
CACCGTGACCGTCGATACCGGGGTTTCACACGGCTTTCCGGGCCTGACTACGGCGCACGCGACCGAATAGGACCGCTTCA
CACGAGATTGGAACATCACA

Downstream 100 bases:

>100_bases
GTGGCTGCAAAGCTGAACATGAAAAAAGGCCGGTCCAGGACCGGCCTTTTGGATTCATAAGAGACCTGAATGATCAGGCG
TTCTCTTCGCCTTCTTCCTG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MAEAASAAHEAVPHRGLITVAIMLGTIMQVLDTTIANVALPSMTGDLGASPDTITWVLTSYIVAAAIMTPVTGWISDRIG
RREFFLVSIAGFTIFSMFCGVAWNLEVMVLFRLLQGVFGAAIVPLAQTFLLDINPKENHGQAMAVFGAGIMVGPIIGPTL
GGWLTETLNWRWVFFINLPVGILCLLGAAAYLPRAARRLRGFDFFGFAMLSLGVGALQLMLDRGGEVDWFASPEIWIELG
LSITGFWLFAVHTLTGENTFIDRHIFRDRNLVTGLVFIFVVGIILLASLALLPPMLSRIFGYTTMLTGLIMAPRGIGTMI
SMIMVGRLVRVIDARILVAAGLSLTAWSLYLMTGFSPQMDGRLIIESGIIQGIGLGLVFVPLSTITFATLDPKYRTDGAS
LFSLTRNIGSSIGISVVTVLLANSTQLNHAELATHITPYNANLWTVMPGAAQGDPTSLSIVDQLVTQQALMISYINDFKF
MMIITLCAIPLVLLLRVPKRKSGSGEAAVAAME

Sequences:

>Translated_513_residues
MAEAASAAHEAVPHRGLITVAIMLGTIMQVLDTTIANVALPSMTGDLGASPDTITWVLTSYIVAAAIMTPVTGWISDRIG
RREFFLVSIAGFTIFSMFCGVAWNLEVMVLFRLLQGVFGAAIVPLAQTFLLDINPKENHGQAMAVFGAGIMVGPIIGPTL
GGWLTETLNWRWVFFINLPVGILCLLGAAAYLPRAARRLRGFDFFGFAMLSLGVGALQLMLDRGGEVDWFASPEIWIELG
LSITGFWLFAVHTLTGENTFIDRHIFRDRNLVTGLVFIFVVGIILLASLALLPPMLSRIFGYTTMLTGLIMAPRGIGTMI
SMIMVGRLVRVIDARILVAAGLSLTAWSLYLMTGFSPQMDGRLIIESGIIQGIGLGLVFVPLSTITFATLDPKYRTDGAS
LFSLTRNIGSSIGISVVTVLLANSTQLNHAELATHITPYNANLWTVMPGAAQGDPTSLSIVDQLVTQQALMISYINDFKF
MMIITLCAIPLVLLLRVPKRKSGSGEAAVAAME
>Mature_512_residues
AEAASAAHEAVPHRGLITVAIMLGTIMQVLDTTIANVALPSMTGDLGASPDTITWVLTSYIVAAAIMTPVTGWISDRIGR
REFFLVSIAGFTIFSMFCGVAWNLEVMVLFRLLQGVFGAAIVPLAQTFLLDINPKENHGQAMAVFGAGIMVGPIIGPTLG
GWLTETLNWRWVFFINLPVGILCLLGAAAYLPRAARRLRGFDFFGFAMLSLGVGALQLMLDRGGEVDWFASPEIWIELGL
SITGFWLFAVHTLTGENTFIDRHIFRDRNLVTGLVFIFVVGIILLASLALLPPMLSRIFGYTTMLTGLIMAPRGIGTMIS
MIMVGRLVRVIDARILVAAGLSLTAWSLYLMTGFSPQMDGRLIIESGIIQGIGLGLVFVPLSTITFATLDPKYRTDGASL
FSLTRNIGSSIGISVVTVLLANSTQLNHAELATHITPYNANLWTVMPGAAQGDPTSLSIVDQLVTQQALMISYINDFKFM
MIITLCAIPLVLLLRVPKRKSGSGEAAVAAME

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=488, Percent_Identity=35.4508196721311, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1788710, Length=497, Percent_Identity=33.1991951710262, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=31.592039800995, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=32.9207920792079, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=28.3333333333333, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87082320, Length=170, Percent_Identity=24.1176470588235, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=386, Percent_Identity=24.3523316062176, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=163, Percent_Identity=24.5398773006135, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321577, Length=135, Percent_Identity=31.8518518518519, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=238, Percent_Identity=25.2100840336134, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325414, Length=135, Percent_Identity=31.8518518518519, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55315; Mature: 55184

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAASAAHEAVPHRGLITVAIMLGTIMQVLDTTIANVALPSMTGDLGASPDTITWVLTS
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
YIVAAAIMTPVTGWISDRIGRREFFLVSIAGFTIFSMFCGVAWNLEVMVLFRLLQGVFGA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIVPLAQTFLLDINPKENHGQAMAVFGAGIMVGPIIGPTLGGWLTETLNWRWVFFINLPV
HHHHHHHHHEEECCCCCCCCCEEEEECCCHHCCCHHCCCHHHHHHHCCCEEEEEEEECCH
GILCLLGAAAYLPRAARRLRGFDFFGFAMLSLGVGALQLMLDRGGEVDWFASPEIWIELG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEC
LSITGFWLFAVHTLTGENTFIDRHIFRDRNLVTGLVFIFVVGIILLASLALLPPMLSRIF
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYTTMLTGLIMAPRGIGTMISMIMVGRLVRVIDARILVAAGLSLTAWSLYLMTGFSPQMD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
GRLIIESGIIQGIGLGLVFVPLSTITFATLDPKYRTDGASLFSLTRNIGSSIGISVVTVL
CEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
LANSTQLNHAELATHITPYNANLWTVMPGAAQGDPTSLSIVDQLVTQQALMISYINDFKF
HHCCCCCCHHHHHHEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MMIITLCAIPLVLLLRVPKRKSGSGEAAVAAME
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
AEAASAAHEAVPHRGLITVAIMLGTIMQVLDTTIANVALPSMTGDLGASPDTITWVLTS
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
YIVAAAIMTPVTGWISDRIGRREFFLVSIAGFTIFSMFCGVAWNLEVMVLFRLLQGVFGA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIVPLAQTFLLDINPKENHGQAMAVFGAGIMVGPIIGPTLGGWLTETLNWRWVFFINLPV
HHHHHHHHHEEECCCCCCCCCEEEEECCCHHCCCHHCCCHHHHHHHCCCEEEEEEEECCH
GILCLLGAAAYLPRAARRLRGFDFFGFAMLSLGVGALQLMLDRGGEVDWFASPEIWIELG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEC
LSITGFWLFAVHTLTGENTFIDRHIFRDRNLVTGLVFIFVVGIILLASLALLPPMLSRIF
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYTTMLTGLIMAPRGIGTMISMIMVGRLVRVIDARILVAAGLSLTAWSLYLMTGFSPQMD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
GRLIIESGIIQGIGLGLVFVPLSTITFATLDPKYRTDGASLFSLTRNIGSSIGISVVTVL
CEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
LANSTQLNHAELATHITPYNANLWTVMPGAAQGDPTSLSIVDQLVTQQALMISYINDFKF
HHCCCCCCHHHHHHEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MMIITLCAIPLVLLLRVPKRKSGSGEAAVAAME
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]