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The map label for this gene is potD-A [H]

Identifier: 110634215

GI number: 110634215

Start: 2004250

End: 2005308

Strand: Reverse

Name: potD-A [H]

Synonym: Meso_1865

Alternate gene names: 110634215

Gene position: 2005308-2004250 (Counterclockwise)

Preceding gene: 110634216

Following gene: 110634214

Centisome position: 45.45

GC content: 59.77

Gene sequence:

>1059_bases
ATGAATGGGAGCAGACAGATGAAATATTGGAACGGTGCAGTAGCTGCGATCGCGGTCCTGATGCTTGGAGCCTCGCCATC
GAGCGCTGAAGACAAGCCGGAAAGCATCATCGTAAACACCTCGGGCGGCGAGAACGGCCCGATGCTCAGGGAAGCCTACT
TCAACGATTTCGAGGCCACGACAGGCATCAAGATCGTCGACTCGAGCCCGGCGGATTTCGGCCGGCTACGCGCGATGGTC
GAGAGCGGCAATGTGGAGTACACAGTCACCGAGGTGGAGTCGGAGGATGCTTGGCGCGCGATCGAGATGAACCTGCTGGA
GCCGATCGACGATGAGATTGTCGACCGCTCAAAATTTCCACCGCAGACGTTGAACCCCTACCTATATCCGATCTCCAGCT
ATTCTACCGTGATCGGCTATTCGACCGAGGCCTTCCCGGAAGGTGGTCCCACGAACTGGAAGGAGTTCTGGGACGTCAAG
ACGTTCCCCGGCCCACGTTCCATGCGCAACCACCCGATCGACAATCTAGAAGCGGCATTGCTTGCCGACGGTGTGGCTCC
GGAAGATCTCTATCCGCTGGATCTGGATAGAGCCTTCGCCAAGCTCGACGAAATCTATCCGCATGTCACGACGTGGTGGA
CCACCGGCGCGCAGCAGGCCCAGCAGCTGATCGACGGAGAGGTCGTGATAACCACCGGCTATAACTCGCGCCTTTACAGC
GCCATCCGCAAGGGCGCCCCGATCAAGATGGAATTTGGCGGCGGCATCCTCAAAGCGGCAGCCTATGCGATCCCAAGGGG
CGCTCCGCACGCTGAATGGGGCCAGAGATTCCTCGCCACGATGGCCAAGCCCGAGAACCAGGGCAAATACGCGCTGGCGT
TCGGCCTCTCCGGCCCGGATCCGGACCACGTGAACTATGTCCCGGAAGACGTGCGCCAGTTGCTTCCGCTGCATCCGGAC
AATCTCAAGAAGCAGGTCTGGATGGATCAGGAGTGGTGGCTGGAGAATGGCGAGGCGGTGTCCGAACGTTGGACCGAGTG
GATGCTGAGCCACAACTGA

Upstream 100 bases:

>100_bases
ATATTGAAGGAAAATGTTATTGCAAAGAATACAATGATAAATGTGGAATTCTATTTGTAAGTTTTCCAACATAATTCGAA
CCATGGGTGTGGACATCAAC

Downstream 100 bases:

>100_bases
CACCGAAGCACAGGCCTCTATGGGTTCAAACTTGTAGGACGTTCCATGCCCGATTTTCGCAATACTTCGGCACTCAAGCT
CGAGAACATTAGGAAGACCT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNGSRQMKYWNGAVAAIAVLMLGASPSSAEDKPESIIVNTSGGENGPMLREAYFNDFEATTGIKIVDSSPADFGRLRAMV
ESGNVEYTVTEVESEDAWRAIEMNLLEPIDDEIVDRSKFPPQTLNPYLYPISSYSTVIGYSTEAFPEGGPTNWKEFWDVK
TFPGPRSMRNHPIDNLEAALLADGVAPEDLYPLDLDRAFAKLDEIYPHVTTWWTTGAQQAQQLIDGEVVITTGYNSRLYS
AIRKGAPIKMEFGGGILKAAAYAIPRGAPHAEWGQRFLATMAKPENQGKYALAFGLSGPDPDHVNYVPEDVRQLLPLHPD
NLKKQVWMDQEWWLENGEAVSERWTEWMLSHN

Sequences:

>Translated_352_residues
MNGSRQMKYWNGAVAAIAVLMLGASPSSAEDKPESIIVNTSGGENGPMLREAYFNDFEATTGIKIVDSSPADFGRLRAMV
ESGNVEYTVTEVESEDAWRAIEMNLLEPIDDEIVDRSKFPPQTLNPYLYPISSYSTVIGYSTEAFPEGGPTNWKEFWDVK
TFPGPRSMRNHPIDNLEAALLADGVAPEDLYPLDLDRAFAKLDEIYPHVTTWWTTGAQQAQQLIDGEVVITTGYNSRLYS
AIRKGAPIKMEFGGGILKAAAYAIPRGAPHAEWGQRFLATMAKPENQGKYALAFGLSGPDPDHVNYVPEDVRQLLPLHPD
NLKKQVWMDQEWWLENGEAVSERWTEWMLSHN
>Mature_352_residues
MNGSRQMKYWNGAVAAIAVLMLGASPSSAEDKPESIIVNTSGGENGPMLREAYFNDFEATTGIKIVDSSPADFGRLRAMV
ESGNVEYTVTEVESEDAWRAIEMNLLEPIDDEIVDRSKFPPQTLNPYLYPISSYSTVIGYSTEAFPEGGPTNWKEFWDVK
TFPGPRSMRNHPIDNLEAALLADGVAPEDLYPLDLDRAFAKLDEIYPHVTTWWTTGAQQAQQLIDGEVVITTGYNSRLYS
AIRKGAPIKMEFGGGILKAAAYAIPRGAPHAEWGQRFLATMAKPENQGKYALAFGLSGPDPDHVNYVPEDVRQLLPLHPD
NLKKQVWMDQEWWLENGEAVSERWTEWMLSHN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39275; Mature: 39275

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGSRQMKYWNGAVAAIAVLMLGASPSSAEDKPESIIVNTSGGENGPMLREAYFNDFEAT
CCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCC
TGIKIVDSSPADFGRLRAMVESGNVEYTVTEVESEDAWRAIEMNLLEPIDDEIVDRSKFP
CCEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHCCCCCC
PQTLNPYLYPISSYSTVIGYSTEAFPEGGPTNWKEFWDVKTFPGPRSMRNHPIDNLEAAL
CCCCCCEEEECCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHH
LADGVAPEDLYPLDLDRAFAKLDEIYPHVTTWWTTGAQQAQQLIDGEVVITTGYNSRLYS
HHCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEECCCHHHHHHHHCCCEEEEECCCHHHHH
AIRKGAPIKMEFGGGILKAAAYAIPRGAPHAEWGQRFLATMAKPENQGKYALAFGLSGPD
HHHCCCCEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
PDHVNYVPEDVRQLLPLHPDNLKKQVWMDQEWWLENGEAVSERWTEWMLSHN
CCCCCCCCHHHHHHCCCCCCCHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGSRQMKYWNGAVAAIAVLMLGASPSSAEDKPESIIVNTSGGENGPMLREAYFNDFEAT
CCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCC
TGIKIVDSSPADFGRLRAMVESGNVEYTVTEVESEDAWRAIEMNLLEPIDDEIVDRSKFP
CCEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHCCCCCC
PQTLNPYLYPISSYSTVIGYSTEAFPEGGPTNWKEFWDVKTFPGPRSMRNHPIDNLEAAL
CCCCCCEEEECCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHH
LADGVAPEDLYPLDLDRAFAKLDEIYPHVTTWWTTGAQQAQQLIDGEVVITTGYNSRLYS
HHCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEECCCHHHHHHHHCCCEEEEECCCHHHHH
AIRKGAPIKMEFGGGILKAAAYAIPRGAPHAEWGQRFLATMAKPENQGKYALAFGLSGPD
HHHCCCCEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
PDHVNYVPEDVRQLLPLHPDNLKKQVWMDQEWWLENGEAVSERWTEWMLSHN
CCCCCCCCHHHHHHCCCCCCCHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]