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The map label for this gene is nifS1 [H]

Identifier: 110634133

GI number: 110634133

Start: 1903747

End: 1904913

Strand: Reverse

Name: nifS1 [H]

Synonym: Meso_1782

Alternate gene names: 110634133

Gene position: 1904913-1903747 (Counterclockwise)

Preceding gene: 110634137

Following gene: 110634132

Centisome position: 43.17

GC content: 63.84

Gene sequence:

>1167_bases
ATGAGTATGGCCGGACAGCGCATCTATCTCGATTACAATGCTACGGCGCCGCTCAGCGAGGCTGCTCGGCGCGCGGTGAT
CGATGCATTTGACGCGGCGAATCCCTCCTCCGTGCATGCTGAAGGCAGGGCGGCGCGGCAGATGGTGGAGGCCGCTCGGC
GCGACGTGGCGGCGCTGGTGAATGGAAAGGCGGCGCATGTGGTCTTCACCTCGGGCGCGACCGAGGCGGCGGCCATGCTC
CTCTCCCCCGACTGGACCATGGGCCGGGCACCCCTCACTTTCTCGCGGCTTTATGTGAGTGCGACCGACCATCCTTGCGT
GCTTTCCGGCGGCCGCTTTGCCGCGGAGACGGTGGAGAAGATACCGGTAGGATCGGACGGGATCATTGATCTTGGCGCGC
TTGAGCGCGCGCTGGCCGCTCATAACGGGACGGAGGGCCTGCCTCTGGTCGCCAGTCATGTGGCGAACAACGAGACCGGC
GTGATCCAGCCGATCGAGGAAATCGGTGCGGTGGTGAAGAGGGCGGGTGGAGTCCCGATCTTCGACGCCGTCCAGGCGGC
AGGACGCATTCCTCTCGATTTATCGGCAGGATATGCCGACTTCTTCATTCTTTCCTCGCATAAGATCGGCGGGCCGAAGG
GCGTGGGCGCGGTTGTGGGCGCGTCCGACCTGATGATGCCCCGCCCCCTGGTGACAGGAGGAGGGCAGGAGAAGGGCCAC
CGCGCCGGAACGGAAAATGTGGCTGCCATCGCGGGTTTCGGTGCGGCCGCCCGCGAAGCGGCGGCGGCCGTTTCCGCCAT
GGATGGCGTTTGCGCGAAGCGCAGCCGCATCGAGGCAATCCTTCGGTCTGTGTTGCCTGATGCGGTGATCTACTGCGAAA
AGGTGCGGCGCCTGCCCAACACTGTCTTCTTTTCCATTCCGGGCATGAAGGCGGAGACGGCGCAGATTGCCTTCGATCTT
GCTGGAATCGCAGTTTCGGCAGGTTCGGCGTGCTCGTCGGGCAAGGTCGGACCGAGCCAGGTGTTGCAAGCGATGGGCTT
CGGCGATCAGGCCGGCGCACTTCGTGTTTCGATCGGCGTTCAGACGACGGATGCGGAAATAGAGGCTTTTGCAGAGGCGC
TCGAAAAAATTGTTGCGAAACGCAAGATGGCGACCGCAGCAGCTTGA

Upstream 100 bases:

>100_bases
GTTGCCGCCCGTTGGCAAGCCGGGTAGATAGGACGGCCTGCCTAGGAAATTCAAGGAAATAACGCCTTCGCCGTCACAAT
TGTGGCAGAAGGTAACATGG

Downstream 100 bases:

>100_bases
ATAATTTGGAGCTTTTTCTGCAAAAGAAAAATGGAGTAAATGGCACATATTTTCTGGCCGCATGGGTGAATTCGATGGGT
GCGCTTCCTAAATAGGGGGC

Product: aminotransferase, class V

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSMAGQRIYLDYNATAPLSEAARRAVIDAFDAANPSSVHAEGRAARQMVEAARRDVAALVNGKAAHVVFTSGATEAAAML
LSPDWTMGRAPLTFSRLYVSATDHPCVLSGGRFAAETVEKIPVGSDGIIDLGALERALAAHNGTEGLPLVASHVANNETG
VIQPIEEIGAVVKRAGGVPIFDAVQAAGRIPLDLSAGYADFFILSSHKIGGPKGVGAVVGASDLMMPRPLVTGGGQEKGH
RAGTENVAAIAGFGAAAREAAAAVSAMDGVCAKRSRIEAILRSVLPDAVIYCEKVRRLPNTVFFSIPGMKAETAQIAFDL
AGIAVSAGSACSSGKVGPSQVLQAMGFGDQAGALRVSIGVQTTDAEIEAFAEALEKIVAKRKMATAAA

Sequences:

>Translated_388_residues
MSMAGQRIYLDYNATAPLSEAARRAVIDAFDAANPSSVHAEGRAARQMVEAARRDVAALVNGKAAHVVFTSGATEAAAML
LSPDWTMGRAPLTFSRLYVSATDHPCVLSGGRFAAETVEKIPVGSDGIIDLGALERALAAHNGTEGLPLVASHVANNETG
VIQPIEEIGAVVKRAGGVPIFDAVQAAGRIPLDLSAGYADFFILSSHKIGGPKGVGAVVGASDLMMPRPLVTGGGQEKGH
RAGTENVAAIAGFGAAAREAAAAVSAMDGVCAKRSRIEAILRSVLPDAVIYCEKVRRLPNTVFFSIPGMKAETAQIAFDL
AGIAVSAGSACSSGKVGPSQVLQAMGFGDQAGALRVSIGVQTTDAEIEAFAEALEKIVAKRKMATAAA
>Mature_387_residues
SMAGQRIYLDYNATAPLSEAARRAVIDAFDAANPSSVHAEGRAARQMVEAARRDVAALVNGKAAHVVFTSGATEAAAMLL
SPDWTMGRAPLTFSRLYVSATDHPCVLSGGRFAAETVEKIPVGSDGIIDLGALERALAAHNGTEGLPLVASHVANNETGV
IQPIEEIGAVVKRAGGVPIFDAVQAAGRIPLDLSAGYADFFILSSHKIGGPKGVGAVVGASDLMMPRPLVTGGGQEKGHR
AGTENVAAIAGFGAAAREAAAAVSAMDGVCAKRSRIEAILRSVLPDAVIYCEKVRRLPNTVFFSIPGMKAETAQIAFDLA
GIAVSAGSACSSGKVGPSQVLQAMGFGDQAGALRVSIGVQTTDAEIEAFAEALEKIVAKRKMATAAA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=399, Percent_Identity=34.5864661654135, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=30.6763285024155, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI48994898, Length=391, Percent_Identity=32.7365728900256, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787970, Length=364, Percent_Identity=23.6263736263736, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789175, Length=407, Percent_Identity=24.3243243243243, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=379, Percent_Identity=33.245382585752, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17533177, Length=336, Percent_Identity=27.3809523809524, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319831, Length=381, Percent_Identity=34.1207349081365, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI20129463, Length=396, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 39717; Mature: 39586

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMAGQRIYLDYNATAPLSEAARRAVIDAFDAANPSSVHAEGRAARQMVEAARRDVAALV
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NGKAAHVVFTSGATEAAAMLLSPDWTMGRAPLTFSRLYVSATDHPCVLSGGRFAAETVEK
CCCEEEEEEECCCCHHHHEEECCCCCCCCCCCEEEEEEEEECCCCEEEECCCHHHHHHHH
IPVGSDGIIDLGALERALAAHNGTEGLPLVASHVANNETGVIQPIEEIGAVVKRAGGVPI
CCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCH
FDAVQAAGRIPLDLSAGYADFFILSSHKIGGPKGVGAVVGASDLMMPRPLVTGGGQEKGH
HHHHHHCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHCCCHHHCCCCCCCCCCCCCCCC
RAGTENVAAIAGFGAAAREAAAAVSAMDGVCAKRSRIEAILRSVLPDAVIYCEKVRRLPN
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVFFSIPGMKAETAQIAFDLAGIAVSAGSACSSGKVGPSQVLQAMGFGDQAGALRVSIGV
EEEEECCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEE
QTTDAEIEAFAEALEKIVAKRKMATAAA
EECHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SMAGQRIYLDYNATAPLSEAARRAVIDAFDAANPSSVHAEGRAARQMVEAARRDVAALV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NGKAAHVVFTSGATEAAAMLLSPDWTMGRAPLTFSRLYVSATDHPCVLSGGRFAAETVEK
CCCEEEEEEECCCCHHHHEEECCCCCCCCCCCEEEEEEEEECCCCEEEECCCHHHHHHHH
IPVGSDGIIDLGALERALAAHNGTEGLPLVASHVANNETGVIQPIEEIGAVVKRAGGVPI
CCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCH
FDAVQAAGRIPLDLSAGYADFFILSSHKIGGPKGVGAVVGASDLMMPRPLVTGGGQEKGH
HHHHHHCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHCCCHHHCCCCCCCCCCCCCCCC
RAGTENVAAIAGFGAAAREAAAAVSAMDGVCAKRSRIEAILRSVLPDAVIYCEKVRRLPN
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVFFSIPGMKAETAQIAFDLAGIAVSAGSACSSGKVGPSQVLQAMGFGDQAGALRVSIGV
EEEEECCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEE
QTTDAEIEAFAEALEKIVAKRKMATAAA
EECHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]