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The map label for this gene is dhfrIII [H]

Identifier: 110634101

GI number: 110634101

Start: 1869998

End: 1870501

Strand: Reverse

Name: dhfrIII [H]

Synonym: Meso_1750

Alternate gene names: 110634101

Gene position: 1870501-1869998 (Counterclockwise)

Preceding gene: 110634102

Following gene: 110634100

Centisome position: 42.39

GC content: 60.12

Gene sequence:

>504_bases
ATGAAGCTTTCTCTCGTCGTGGCTGTCGCCAATAACGGCGTCATCGGCCACCAAGGTGATTTGCCGTGGCGGTTGTCCAC
AGATCTGAAGCGCTTCAAAGCCGTGACCATGGGCAAGCCCATAATCATGGGGCGAAAGACCTGGCAGAGCATAGGGAAGC
CGCTCCCGGGACGCCAGAATATCGTGATTAGCCGCAATCCCGCTTTCACGGCGGAAGGGGCGTATGCCGCCGGTTCGCTT
GACGAGGCGATCGCGCTGGCCGGCCGGCAGGACGGTATCGAGGAGATCTGCGTCATCGGAGGCGCAGAAATTTACCGGCA
GGCGCTTTCTCGCGCCGATATCCTCCATGTTACTCATGTCGAGGCCGACGTGGAGGGCGATGCCGTTTTTCCCGAAATCG
ATCCGCAGGTTTGGCGGCCCGTTCATAGCGAAAGCGTGCCCGCCGGCGAAAAGGATGTTTACCCAACGCGCTATGTGATT
TACGAGCGGCGGCAGGCGGTCTGA

Upstream 100 bases:

>100_bases
TTCGCCTTTGGCTACGAGGATTTTCGTTTGGAGGGCTATGTGGCCGATCCGACGATCCGCGCGCCCATCGCCGTATAGGC
CAACAGATTGCGAGCGTCGA

Downstream 100 bases:

>100_bases
TGCGGATTTGGGAAGCGGCGGCATGAAATGGCCGCACGACATTGAGCGGCTGCGTTGAAAGCCTGCTTCCCGACACCTAT
AAATGGCCGAACGGCAATGT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MKLSLVVAVANNGVIGHQGDLPWRLSTDLKRFKAVTMGKPIIMGRKTWQSIGKPLPGRQNIVISRNPAFTAEGAYAAGSL
DEAIALAGRQDGIEEICVIGGAEIYRQALSRADILHVTHVEADVEGDAVFPEIDPQVWRPVHSESVPAGEKDVYPTRYVI
YERRQAV

Sequences:

>Translated_167_residues
MKLSLVVAVANNGVIGHQGDLPWRLSTDLKRFKAVTMGKPIIMGRKTWQSIGKPLPGRQNIVISRNPAFTAEGAYAAGSL
DEAIALAGRQDGIEEICVIGGAEIYRQALSRADILHVTHVEADVEGDAVFPEIDPQVWRPVHSESVPAGEKDVYPTRYVI
YERRQAV
>Mature_167_residues
MKLSLVVAVANNGVIGHQGDLPWRLSTDLKRFKAVTMGKPIIMGRKTWQSIGKPLPGRQNIVISRNPAFTAEGAYAAGSL
DEAIALAGRQDGIEEICVIGGAEIYRQALSRADILHVTHVEADVEGDAVFPEIDPQVWRPVHSESVPAGEKDVYPTRYVI
YERRQAV

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=154, Percent_Identity=35.7142857142857, Blast_Score=87, Evalue=5e-18,
Organism=Homo sapiens, GI28827793, Length=154, Percent_Identity=36.3636363636364, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=45.0617283950617, Blast_Score=140, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17505899, Length=177, Percent_Identity=33.8983050847458, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324810, Length=164, Percent_Identity=33.5365853658537, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116008026, Length=180, Percent_Identity=31.6666666666667, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647458, Length=180, Percent_Identity=31.6666666666667, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18271; Mature: 18271

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSLVVAVANNGVIGHQGDLPWRLSTDLKRFKAVTMGKPIIMGRKTWQSIGKPLPGRQN
CCEEEEEEECCCCEECCCCCCCEEECHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRNPAFTAEGAYAAGSLDEAIALAGRQDGIEEICVIGGAEIYRQALSRADILHVTHV
EEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHEECCHHHHHHHHHHCCEEEEEEE
EADVEGDAVFPEIDPQVWRPVHSESVPAGEKDVYPTRYVIYERRQAV
ECCCCCCEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKLSLVVAVANNGVIGHQGDLPWRLSTDLKRFKAVTMGKPIIMGRKTWQSIGKPLPGRQN
CCEEEEEEECCCCEECCCCCCCEEECHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRNPAFTAEGAYAAGSLDEAIALAGRQDGIEEICVIGGAEIYRQALSRADILHVTHV
EEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHEECCHHHHHHHHHHCCEEEEEEE
EADVEGDAVFPEIDPQVWRPVHSESVPAGEKDVYPTRYVIYERRQAV
ECCCCCCEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]