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The map label for this gene is rne [H]

Identifier: 110634063

GI number: 110634063

Start: 1833893

End: 1836499

Strand: Direct

Name: rne [H]

Synonym: Meso_1711

Alternate gene names: 110634063

Gene position: 1833893-1836499 (Clockwise)

Preceding gene: 110634056

Following gene: 110634064

Centisome position: 41.56

GC content: 62.1

Gene sequence:

>2607_bases
ATGCCAAACAAGATGCTGATAGACGCCTCCCACCCGGAGGAAACCCGCGTTGTTGTCCTGCGCGGTAACCGTATCGAAGA
GTTCGATTTCGAATCACAAGATAAGAAGCAACTCAGAGGAAACATCTACCTTGCGCGGGTGACACGCGTAGAGCCTTCCC
TGCAGGCTGCCTTTGTTGAATACGGAGGAAACCGCCACGGCTTCCTCGCCTTCAGCGAAATTCATCCTGATTATTACCAG
ATTCCCGTTGCCGATCGGCAGGCGCTTCTGGAAGCCGAAGCCGAGGAAGCCCGCGCGGCCGACGAAGCGGAGGAAGGCGA
GGCGCAGGCCGGGCGCGGCCGCAAGCGTCGTCGTCGTGATGCTAAGACCGACGAGGTTGTGTCCGAAGGCAAGGATGTGG
CGCAGCCGGAAGCTGTCAGGGACGATACCGTCTCCGAGCCGCTTGCTGCCGATGGCAACGGCTCGGATGAAGCGAACGGA
TCGGATGAGGTCGATACGGTTGGCGCCGAGGACGCGCTTGATGAAGCGCAGAATCGCCGGCGCCCCGTGCGTCGCAACTA
TAAGATCCAGGAAGTGATCAAGCGCCGGCAAATCATGCTGGTGCAGGTGGTTAAGGAAGAACGCGGCAGCAAGGGCGCGG
CTCTCACCACCTATCTGTCGCTCGCCGGACGGTATTCCGTGCTGATGCCGAATACCGCGCGCGGCGGCGGCATCTCGCGC
AAGATCACGAATCTCCAGGACCGCAAGCGGCTCAAAGAGGTGGTCAAGGGCCTCGATGTACCGGAAGGCATGGGGGTAAT
CCTTCGCACGGCCGGTGCCAATCGCACCAAGGCTGAAATCAAGCGCGACTACGAATATCTGATGCGGCTTTGGGAGCGGG
TGCGCACTTTGACGCTCGAATCCATAGCCCCTGCCCTCGTTTATGAGGAAGGCAGCCTCATCAAGCGCGCGGTGCGCGAT
CTCTACAACAAGGATATCGACGAAATCCTGGTGGCCGGTGAAGACGGCTATCGCGAGGCCAAGGATTTCATGCGCATGCT
GATGCCGAGCCATGCGAAAGCTGTGCAGCCATACCGCGACAGCGTGCCGATTTTCGCGCGCAACGGCATCGAAGCTCAGT
TGGATAAGATGCTGCAGCCGCAGGTGACGCTAAAGTCAGGCGGCTATATCATCATCAACCAGACTGAAGCCCTTGTCGCC
ATCGACGTAAACTCCGGCCGCTCGACAAAGGAGCATTCCATCGAGGACACCGCCCTCCAGACCAATCTGGAGGCGGCGGA
AGAGATATCGAGACAGCTTCGCCTGCGCGATCTTGCGGGACTGATCGTCATCGACTTCATCGACATGGAGGAGAACCGCA
ACAACCGCGCGGTCGAAAAGCGTCTGAAAGAATGTCTGAAGAGCGACCGCGCCCGGATCCAGGTGGGGCGCATCTCGCAT
TTCGGCCTGCTGGAGATGTCGCGCCAGCGCATCCGTTCAAGCGTTCTGGAAAGCACAATGCAGCCCTGCCCCCATTGCGG
CGGCACCGGCCATGTGCGTTCCGATTCGTCTGTTGCGCTGCTCGTGGTGCGCGCCATCGAGGAATATCTGTTGAAGGATG
GACGCCACGATATCACCGTGCGCACCCCCACTTCGACGGCTCTCTACCTTCTCAATCACAAGCGCGCGACGCTCGTCGAG
ATGGAGTCGCGGTTTGGCGTGGAGATCACGATCGAGACGGACGAGGAGTTGGGAACCCAGCACTACGCCATTGTAAAAGG
CCAGCCCTCCACCCGCGTTCCGGCGTCGAGAGCGGTCGTCCAGGCCGTGCCGGAAGTCGAGGATGAAGCGGAGGAGGAAA
CCTACGTCGAAGAGACGGAGGAACTGGAGGAGAAGCAGACGGCAGCCGAGGAGCCGGGCGGTCCTGGCCGCAAGCGGCGC
CGCCGCCGCCGTCGGCGTGGAGGAAAGGACCGTGCCGCCGAGCAAGCTGCGCCGCATTCCGCGGTAACAGAGGAAATCTC
GGAGCCGGCAGCCGAGACGGGCGAGGCCGATCTCACCGCCGCCGAAACGGAAGCGGAGGCGGCAGACGTCGAGGCTGGCG
ACGGTGGACCTCGCAAGAAGCGCCACCGCGGAAAGCGCGGCGGCAAGCGCAATCGCCGGGACGAGGAAGCCGCACCGGAA
GATGCGGTAACGGCGCAGTCAGAGGAACCGGCGGGCGAAATTCAGGCAGAGGCGGCGAGCGAAGCCGTGTCGAGCGAAGC
GGCAGCGGCGACGAGCGAAGCGGCAGCGGCGGAGGCAGAGCCTGTTGCGGAGGTTTCCGCCGAAGAAGTGGTGGTGGAAG
AGAAACCAGCCAAGAAACCGCGCCGAAAGACCCGCGCGAAAAAGGATGCGGCTGCCGACGAGATCGCGCAGCCAGCCCCG
GTGGCGCAGGACGAAGAACCCGCGCCAGCCGCCCCAACGGTCGAAGCTCTGGTCGAGGACAAGCCCGAAGACAAGCCGTC
CAAGCCATCAAGGCGAACCGCTGCCAACACTTCGGAAAAGCCAAAACCCGTCGTTACATCTACCGGGGAAAGCGGTGAAG
CACCGGCGCGAAAGACCGGGTGGTGGCAGCGCAAGAGCTTCTTTTAG

Upstream 100 bases:

>100_bases
GCAGGCTTCAGATTGTCCGGCATGAGAATCCAGAAGAGCCAGGAAGAGAAGCGGAACGCTTCCATCCGGCACTGGATTCT
TGCCGGGGAGAAAAGATTTT

Downstream 100 bases:

>100_bases
GACGAGGTAGCAACGAAATAGCCGGGCACCCTGCCCGGCTATTCTCCTTGAAAATGTCCACAGCGCCCCACGCTCCTTAG
GACGGCACACAAGGATGCAG

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 868; Mature: 867

Protein sequence:

>868_residues
MPNKMLIDASHPEETRVVVLRGNRIEEFDFESQDKKQLRGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
IPVADRQALLEAEAEEARAADEAEEGEAQAGRGRKRRRRDAKTDEVVSEGKDVAQPEAVRDDTVSEPLAADGNGSDEANG
SDEVDTVGAEDALDEAQNRRRPVRRNYKIQEVIKRRQIMLVQVVKEERGSKGAALTTYLSLAGRYSVLMPNTARGGGISR
KITNLQDRKRLKEVVKGLDVPEGMGVILRTAGANRTKAEIKRDYEYLMRLWERVRTLTLESIAPALVYEEGSLIKRAVRD
LYNKDIDEILVAGEDGYREAKDFMRMLMPSHAKAVQPYRDSVPIFARNGIEAQLDKMLQPQVTLKSGGYIIINQTEALVA
IDVNSGRSTKEHSIEDTALQTNLEAAEEISRQLRLRDLAGLIVIDFIDMEENRNNRAVEKRLKECLKSDRARIQVGRISH
FGLLEMSRQRIRSSVLESTMQPCPHCGGTGHVRSDSSVALLVVRAIEEYLLKDGRHDITVRTPTSTALYLLNHKRATLVE
MESRFGVEITIETDEELGTQHYAIVKGQPSTRVPASRAVVQAVPEVEDEAEEETYVEETEELEEKQTAAEEPGGPGRKRR
RRRRRRGGKDRAAEQAAPHSAVTEEISEPAAETGEADLTAAETEAEAADVEAGDGGPRKKRHRGKRGGKRNRRDEEAAPE
DAVTAQSEEPAGEIQAEAASEAVSSEAAAATSEAAAAEAEPVAEVSAEEVVVEEKPAKKPRRKTRAKKDAAADEIAQPAP
VAQDEEPAPAAPTVEALVEDKPEDKPSKPSRRTAANTSEKPKPVVTSTGESGEAPARKTGWWQRKSFF

Sequences:

>Translated_868_residues
MPNKMLIDASHPEETRVVVLRGNRIEEFDFESQDKKQLRGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
IPVADRQALLEAEAEEARAADEAEEGEAQAGRGRKRRRRDAKTDEVVSEGKDVAQPEAVRDDTVSEPLAADGNGSDEANG
SDEVDTVGAEDALDEAQNRRRPVRRNYKIQEVIKRRQIMLVQVVKEERGSKGAALTTYLSLAGRYSVLMPNTARGGGISR
KITNLQDRKRLKEVVKGLDVPEGMGVILRTAGANRTKAEIKRDYEYLMRLWERVRTLTLESIAPALVYEEGSLIKRAVRD
LYNKDIDEILVAGEDGYREAKDFMRMLMPSHAKAVQPYRDSVPIFARNGIEAQLDKMLQPQVTLKSGGYIIINQTEALVA
IDVNSGRSTKEHSIEDTALQTNLEAAEEISRQLRLRDLAGLIVIDFIDMEENRNNRAVEKRLKECLKSDRARIQVGRISH
FGLLEMSRQRIRSSVLESTMQPCPHCGGTGHVRSDSSVALLVVRAIEEYLLKDGRHDITVRTPTSTALYLLNHKRATLVE
MESRFGVEITIETDEELGTQHYAIVKGQPSTRVPASRAVVQAVPEVEDEAEEETYVEETEELEEKQTAAEEPGGPGRKRR
RRRRRRGGKDRAAEQAAPHSAVTEEISEPAAETGEADLTAAETEAEAADVEAGDGGPRKKRHRGKRGGKRNRRDEEAAPE
DAVTAQSEEPAGEIQAEAASEAVSSEAAAATSEAAAAEAEPVAEVSAEEVVVEEKPAKKPRRKTRAKKDAAADEIAQPAP
VAQDEEPAPAAPTVEALVEDKPEDKPSKPSRRTAANTSEKPKPVVTSTGESGEAPARKTGWWQRKSFF
>Mature_867_residues
PNKMLIDASHPEETRVVVLRGNRIEEFDFESQDKKQLRGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQI
PVADRQALLEAEAEEARAADEAEEGEAQAGRGRKRRRRDAKTDEVVSEGKDVAQPEAVRDDTVSEPLAADGNGSDEANGS
DEVDTVGAEDALDEAQNRRRPVRRNYKIQEVIKRRQIMLVQVVKEERGSKGAALTTYLSLAGRYSVLMPNTARGGGISRK
ITNLQDRKRLKEVVKGLDVPEGMGVILRTAGANRTKAEIKRDYEYLMRLWERVRTLTLESIAPALVYEEGSLIKRAVRDL
YNKDIDEILVAGEDGYREAKDFMRMLMPSHAKAVQPYRDSVPIFARNGIEAQLDKMLQPQVTLKSGGYIIINQTEALVAI
DVNSGRSTKEHSIEDTALQTNLEAAEEISRQLRLRDLAGLIVIDFIDMEENRNNRAVEKRLKECLKSDRARIQVGRISHF
GLLEMSRQRIRSSVLESTMQPCPHCGGTGHVRSDSSVALLVVRAIEEYLLKDGRHDITVRTPTSTALYLLNHKRATLVEM
ESRFGVEITIETDEELGTQHYAIVKGQPSTRVPASRAVVQAVPEVEDEAEEETYVEETEELEEKQTAAEEPGGPGRKRRR
RRRRRGGKDRAAEQAAPHSAVTEEISEPAAETGEADLTAAETEAEAADVEAGDGGPRKKRHRGKRGGKRNRRDEEAAPED
AVTAQSEEPAGEIQAEAASEAVSSEAAAATSEAAAAEAEPVAEVSAEEVVVEEKPAKKPRRKTRAKKDAAADEIAQPAPV
AQDEEPAPAAPTVEALVEDKPEDKPSKPSRRTAANTSEKPKPVVTSTGESGEAPARKTGWWQRKSFF

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=401, Percent_Identity=46.3840399002494, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI87082235, Length=338, Percent_Identity=37.2781065088757, Blast_Score=198, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 95964; Mature: 95833

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKMLIDASHPEETRVVVLRGNRIEEFDFESQDKKQLRGNIYLARVTRVEPSLQAAFVE
CCCCEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQIPVADRQALLEAEAEEARAADEAEEGEAQAGRGRKRRRRD
HCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHCCHHHCCCHHHHHHC
AKTDEVVSEGKDVAQPEAVRDDTVSEPLAADGNGSDEANGSDEVDTVGAEDALDEAQNRR
CHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
RPVRRNYKIQEVIKRRQIMLVQVVKEERGSKGAALTTYLSLAGRYSVLMPNTARGGGISR
CCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCHH
KITNLQDRKRLKEVVKGLDVPEGMGVILRTAGANRTKAEIKRDYEYLMRLWERVRTLTLE
HHHCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SIAPALVYEEGSLIKRAVRDLYNKDIDEILVAGEDGYREAKDFMRMLMPSHAKAVQPYRD
HHCCHHEECCHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHCCCHHHHHCCHHC
SVPIFARNGIEAQLDKMLQPQVTLKSGGYIIINQTEALVAIDVNSGRSTKEHSIEDTALQ
CCCEEECCCCHHHHHHHCCCCEEEECCCEEEEECCCEEEEEECCCCCCCCCCCCHHHHHH
TNLEAAEEISRQLRLRDLAGLIVIDFIDMEENRNNRAVEKRLKECLKSDRARIQVGRISH
HHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEEEECCCCH
FGLLEMSRQRIRSSVLESTMQPCPHCGGTGHVRSDSSVALLVVRAIEEYLLKDGRHDITV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
RTPTSTALYLLNHKRATLVEMESRFGVEITIETDEELGTQHYAIVKGQPSTRVPASRAVV
ECCCCEEEEEEECCCHHEEEHHHHCCEEEEEECCHHHCCCEEEEEECCCCCCCCHHHHHH
QAVPEVEDEAEEETYVEETEELEEKQTAAEEPGGPGRKRRRRRRRRGGKDRAAEQAAPHS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCCHH
AVTEEISEPAAETGEADLTAAETEAEAADVEAGDGGPRKKRHRGKRGGKRNRRDEEAAPE
HHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
DAVTAQSEEPAGEIQAEAASEAVSSEAAAATSEAAAAEAEPVAEVSAEEVVVEEKPAKKP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHEECCCCCCCH
RRKTRAKKDAAADEIAQPAPVAQDEEPAPAAPTVEALVEDKPEDKPSKPSRRTAANTSEK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PKPVVTSTGESGEAPARKTGWWQRKSFF
CCCCEECCCCCCCCCCHHCCCCHHCCCC
>Mature Secondary Structure 
PNKMLIDASHPEETRVVVLRGNRIEEFDFESQDKKQLRGNIYLARVTRVEPSLQAAFVE
CCCEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQIPVADRQALLEAEAEEARAADEAEEGEAQAGRGRKRRRRD
HCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHCCHHHCCCHHHHHHC
AKTDEVVSEGKDVAQPEAVRDDTVSEPLAADGNGSDEANGSDEVDTVGAEDALDEAQNRR
CHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
RPVRRNYKIQEVIKRRQIMLVQVVKEERGSKGAALTTYLSLAGRYSVLMPNTARGGGISR
CCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCHH
KITNLQDRKRLKEVVKGLDVPEGMGVILRTAGANRTKAEIKRDYEYLMRLWERVRTLTLE
HHHCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SIAPALVYEEGSLIKRAVRDLYNKDIDEILVAGEDGYREAKDFMRMLMPSHAKAVQPYRD
HHCCHHEECCHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHCCCHHHHHCCHHC
SVPIFARNGIEAQLDKMLQPQVTLKSGGYIIINQTEALVAIDVNSGRSTKEHSIEDTALQ
CCCEEECCCCHHHHHHHCCCCEEEECCCEEEEECCCEEEEEECCCCCCCCCCCCHHHHHH
TNLEAAEEISRQLRLRDLAGLIVIDFIDMEENRNNRAVEKRLKECLKSDRARIQVGRISH
HHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEEEECCCCH
FGLLEMSRQRIRSSVLESTMQPCPHCGGTGHVRSDSSVALLVVRAIEEYLLKDGRHDITV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
RTPTSTALYLLNHKRATLVEMESRFGVEITIETDEELGTQHYAIVKGQPSTRVPASRAVV
ECCCCEEEEEEECCCHHEEEHHHHCCEEEEEECCHHHCCCEEEEEECCCCCCCCHHHHHH
QAVPEVEDEAEEETYVEETEELEEKQTAAEEPGGPGRKRRRRRRRRGGKDRAAEQAAPHS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCCHH
AVTEEISEPAAETGEADLTAAETEAEAADVEAGDGGPRKKRHRGKRGGKRNRRDEEAAPE
HHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
DAVTAQSEEPAGEIQAEAASEAVSSEAAAATSEAAAAEAEPVAEVSAEEVVVEEKPAKKP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHEECCCCCCCH
RRKTRAKKDAAADEIAQPAPVAQDEEPAPAAPTVEALVEDKPEDKPSKPSRRTAANTSEK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PKPVVTSTGESGEAPARKTGWWQRKSFF
CCCCEECCCCCCCCCCHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]