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The map label for this gene is ispDF

Identifier: 110633973

GI number: 110633973

Start: 1747305

End: 1748531

Strand: Direct

Name: ispDF

Synonym: Meso_1621

Alternate gene names: 110633973

Gene position: 1747305-1748531 (Clockwise)

Preceding gene: 110633963

Following gene: 110633974

Centisome position: 39.6

GC content: 60.55

Gene sequence:

>1227_bases
ATGAACGCACCTTTTGAAAAGGATAGGCGCAACCGTCGAGTTGCCGTTGTGGTGGTTGCTGCAGGGCGCGGCGAGCGGGC
CGGCCAGGCCCAGAACGGCCCGAAACAATACCGGCTGATTGGCGGCAAGCCTGTCATCCGCCACACACTTGAAGTCTTGG
CGAAGAGCAAACGCGTCGGCACAATCGCCGTTGCCGTCCATCCAGAGGATAGCTCCCTCTTCAACCAGGCACGCGAAGGA
CTGGATGGCGATATCCGCGCCGTTCATGGCGGAGCCTCACGGCAGGAATCGACGCGGCTTGCGCTTGCCGCGCTCGAAGA
GGTGAAGCCTGATATCGTGCTTATCCATGACGGCGTAAGGCCATTCATCGATGAAGCATTGATTGAGCGCGTCATAGAGG
CCGTGGGTGAGGACAGCGGTGCCCTTCCCGCACTTCCGGTGTCCGACACGCTGAAACGTTCGGGTGTGAACGGCATCATA
ACGGGCACCGTGCCGCGCGACGGTCTGTTTGCCGCTCAGACTCCTCAAGGCTTCCCTTTTCGCCCGATCTATGAGGCACA
CCAGAAAGCTTGGCAGTCCGGCCGTTCGGATTTCACGGACGATGCAGCTATCGCTGAATGGGCAGGCATTTCGGTCCGCC
TTGTCAGCGGCTCGCCGGACAATGTGAAGCTCACATGGGCCAAGGATATTGCTCTGGCCGACCAAAGGTTAGGACCCGCC
ATGACGGAGTTGCCGGATATCCGGGTAGGCAATGGATATGATGTGCATGGGTTCGGTCCGGGTGGCCATGTAACTCTGTG
CGGTGTTCAAATCCCTCACGGCAAAACCCTGATGGGTCATTCCGATGCGGATGTGGGTCTGCACGCCCTTACAGATGCGC
TGCTCGCCACCTGTGGGGCCGGCGATATAGGCACCCACTTTCCGCCCTCGGACCCGCAATGGAAAGGCGCAAAATCCCGC
ATTTTTGTCGAACACGCGGTCAATCTCCTACGTGCGCGCGGCGGGCGCATCGCCAATGCTGATGTCACGCTCATTTGCGA
GGCGCCGCGCATCGGGCCTTATCGCGAGGCCATGCTGGCGGAACTTTCGGATATGTTGCAGATTTCGCCGGAGCGCGTTT
CCGTCAAGGCGACTACCAATGAGGGGCTCGGTTTCATTGGCCGGGCCGAGGGGATCGCGGCGATCGCCACGGCTACGGTC
GCCTACCCTGGCTCCCTGGGAAACTGA

Upstream 100 bases:

>100_bases
CCCATTTGTTAGACATTTCGTGCGTGTTGCGCAATAGCTGCGTTGTAATGGCAGAGAATCCGGTAGCTTTTCAACCTTGC
CGCAATTATGCGTCTTCGGC

Downstream 100 bases:

>100_bases
TCGGCGGAGCTTTCGATGGAAGATAGCGAAGAACTGGCACGGGAGGTTCTACGCGTTTTCGAAGAGCGCGACTTGAAGCT
CGCCACCGCCGAATCCTGCA

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNAPFEKDRRNRRVAVVVVAAGRGERAGQAQNGPKQYRLIGGKPVIRHTLEVLAKSKRVGTIAVAVHPEDSSLFNQAREG
LDGDIRAVHGGASRQESTRLALAALEEVKPDIVLIHDGVRPFIDEALIERVIEAVGEDSGALPALPVSDTLKRSGVNGII
TGTVPRDGLFAAQTPQGFPFRPIYEAHQKAWQSGRSDFTDDAAIAEWAGISVRLVSGSPDNVKLTWAKDIALADQRLGPA
MTELPDIRVGNGYDVHGFGPGGHVTLCGVQIPHGKTLMGHSDADVGLHALTDALLATCGAGDIGTHFPPSDPQWKGAKSR
IFVEHAVNLLRARGGRIANADVTLICEAPRIGPYREAMLAELSDMLQISPERVSVKATTNEGLGFIGRAEGIAAIATATV
AYPGSLGN

Sequences:

>Translated_408_residues
MNAPFEKDRRNRRVAVVVVAAGRGERAGQAQNGPKQYRLIGGKPVIRHTLEVLAKSKRVGTIAVAVHPEDSSLFNQAREG
LDGDIRAVHGGASRQESTRLALAALEEVKPDIVLIHDGVRPFIDEALIERVIEAVGEDSGALPALPVSDTLKRSGVNGII
TGTVPRDGLFAAQTPQGFPFRPIYEAHQKAWQSGRSDFTDDAAIAEWAGISVRLVSGSPDNVKLTWAKDIALADQRLGPA
MTELPDIRVGNGYDVHGFGPGGHVTLCGVQIPHGKTLMGHSDADVGLHALTDALLATCGAGDIGTHFPPSDPQWKGAKSR
IFVEHAVNLLRARGGRIANADVTLICEAPRIGPYREAMLAELSDMLQISPERVSVKATTNEGLGFIGRAEGIAAIATATV
AYPGSLGN
>Mature_408_residues
MNAPFEKDRRNRRVAVVVVAAGRGERAGQAQNGPKQYRLIGGKPVIRHTLEVLAKSKRVGTIAVAVHPEDSSLFNQAREG
LDGDIRAVHGGASRQESTRLALAALEEVKPDIVLIHDGVRPFIDEALIERVIEAVGEDSGALPALPVSDTLKRSGVNGII
TGTVPRDGLFAAQTPQGFPFRPIYEAHQKAWQSGRSDFTDDAAIAEWAGISVRLVSGSPDNVKLTWAKDIALADQRLGPA
MTELPDIRVGNGYDVHGFGPGGHVTLCGVQIPHGKTLMGHSDADVGLHALTDALLATCGAGDIGTHFPPSDPQWKGAKSR
IFVEHAVNLLRARGGRIANADVTLICEAPRIGPYREAMLAELSDMLQISPERVSVKATTNEGLGFIGRAEGIAAIATATV
AYPGSLGN

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=238, Percent_Identity=31.5126050420168, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI157671913, Length=140, Percent_Identity=34.2857142857143, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=47.4025974025974, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=36.4035087719298, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_MESSB (Q11HV9)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674181.1
- ProteinModelPortal:   Q11HV9
- SMR:   Q11HV9
- STRING:   Q11HV9
- GeneID:   4179827
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1621
- NMPDR:   fig|266779.1.peg.367
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q11HV9
- ProtClustDB:   PRK09382
- BioCyc:   MSP266779:MESO_1621-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 43220; Mature: 43220

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPFEKDRRNRRVAVVVVAAGRGERAGQAQNGPKQYRLIGGKPVIRHTLEVLAKSKRVG
CCCCCCCHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCE
TIAVAVHPEDSSLFNQAREGLDGDIRAVHGGASRQESTRLALAALEEVKPDIVLIHDGVR
EEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCH
PFIDEALIERVIEAVGEDSGALPALPVSDTLKRSGVNGIITGTVPRDGLFAAQTPQGFPF
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCEEECCCCCCCCC
RPIYEAHQKAWQSGRSDFTDDAAIAEWAGISVRLVSGSPDNVKLTWAKDIALADQRLGPA
CHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEECCCCCEEEEEECCHHHHHHHCCCH
MTELPDIRVGNGYDVHGFGPGGHVTLCGVQIPHGKTLMGHSDADVGLHALTDALLATCGA
HHCCCCEECCCCEEECEECCCCCEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCC
GDIGTHFPPSDPQWKGAKSRIFVEHAVNLLRARGGRIANADVTLICEAPRIGPYREAMLA
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHH
ELSDMLQISPERVSVKATTNEGLGFIGRAEGIAAIATATVAYPGSLGN
HHHHHHHCCCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MNAPFEKDRRNRRVAVVVVAAGRGERAGQAQNGPKQYRLIGGKPVIRHTLEVLAKSKRVG
CCCCCCCHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCE
TIAVAVHPEDSSLFNQAREGLDGDIRAVHGGASRQESTRLALAALEEVKPDIVLIHDGVR
EEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCH
PFIDEALIERVIEAVGEDSGALPALPVSDTLKRSGVNGIITGTVPRDGLFAAQTPQGFPF
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCEEECCCCCCCCC
RPIYEAHQKAWQSGRSDFTDDAAIAEWAGISVRLVSGSPDNVKLTWAKDIALADQRLGPA
CHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEECCCCCEEEEEECCHHHHHHHCCCH
MTELPDIRVGNGYDVHGFGPGGHVTLCGVQIPHGKTLMGHSDADVGLHALTDALLATCGA
HHCCCCEECCCCEEECEECCCCCEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCC
GDIGTHFPPSDPQWKGAKSRIFVEHAVNLLRARGGRIANADVTLICEAPRIGPYREAMLA
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHH
ELSDMLQISPERVSVKATTNEGLGFIGRAEGIAAIATATVAYPGSLGN
HHHHHHHCCCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA