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The map label for this gene is hisJ [H]

Identifier: 110633915

GI number: 110633915

Start: 1680576

End: 1681361

Strand: Direct

Name: hisJ [H]

Synonym: Meso_1562

Alternate gene names: 110633915

Gene position: 1680576-1681361 (Clockwise)

Preceding gene: 110633914

Following gene: 110633916

Centisome position: 38.09

GC content: 56.62

Gene sequence:

>786_bases
ATGCGTATCACCGGACGTATTGCACTCATTCTTTCCGCCGCGCTCGTGGCACTGTCGGCCAGCGCCTCCCAAGCCCAGGA
AAGGATGCAGCTAAAAATCGGAACCGAGGGCGCCTACCCACCCTTCAATAATCTCACCGCCGATGGAAACCTCGAAGGGT
TTGATATCGATATCGCCCGCGCGCTCTGCGAGGAGATGAATGCGGAATGCGAATTCGTCACGCAGGAGTGGGATGGCATG
ATCCCCGCGCTTCAGGCAGGCAAATTCGACGCCATCATCGCTTCTATGTCGATCACTGACGAACGCAAGCAGCAGGTCGA
CTTTACAGAGAAATATTACAACACGCCGCCGGCTATCGTCGTGGCGAAAGATTCTGATATCGCCGGCGTGACGAAGGAAG
ATCTCGCTGGCAGGACGATCGGCGCCCAAGCGTCCACGACGCATGGCAATTTTGCCGAGGCGACTTTCCAGGAAAGCGAG
GTCAAGCTCTATCCGACCGCCGAGGAATACAAGCTCGACATGACGAACGGGCGTCTGGACGCTGTGGTCGATGACGTAAT
CGTCCTGCAGGACTGGCTAAGCACGCCCGATGGGGAGTGCTGTAAGGTGCTGGGCACGCTCACGCCTGTCGAGGAAATCT
ACGGCGAGGGAATCGGCATAGCCATCCGCAAGGGCGAGGATGAGCTGCGCGAGAAATTCAACGCCGCTATCCAAGCCATC
CGCGAAAACGGAAAATACAAGGAAATCAATGACAAGTACTTCGAGTTCGACGTTTACGGCGGTTAG

Upstream 100 bases:

>100_bases
CTTACTCTTTCCCGTTGCATTTCCTTCCGAAAGAATGGGAATATCAGCTCCAAAGCGGGCGCCTGAGAAGGTGACCGGAA
CAAGAACAGTGAGGGAGATG

Downstream 100 bases:

>100_bases
GCTGCCGCGGGCGGGAGAAATCCCGTCGCTTTACCTGCGCAAGCCGACGCAGTTCAACACGCGGGTTCCCGCTGAAGAAG
CCAACTGGCGTCGTTGGCAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRITGRIALILSAALVALSASASQAQERMQLKIGTEGAYPPFNNLTADGNLEGFDIDIARALCEEMNAECEFVTQEWDGM
IPALQAGKFDAIIASMSITDERKQQVDFTEKYYNTPPAIVVAKDSDIAGVTKEDLAGRTIGAQASTTHGNFAEATFQESE
VKLYPTAEEYKLDMTNGRLDAVVDDVIVLQDWLSTPDGECCKVLGTLTPVEEIYGEGIGIAIRKGEDELREKFNAAIQAI
RENGKYKEINDKYFEFDVYGG

Sequences:

>Translated_261_residues
MRITGRIALILSAALVALSASASQAQERMQLKIGTEGAYPPFNNLTADGNLEGFDIDIARALCEEMNAECEFVTQEWDGM
IPALQAGKFDAIIASMSITDERKQQVDFTEKYYNTPPAIVVAKDSDIAGVTKEDLAGRTIGAQASTTHGNFAEATFQESE
VKLYPTAEEYKLDMTNGRLDAVVDDVIVLQDWLSTPDGECCKVLGTLTPVEEIYGEGIGIAIRKGEDELREKFNAAIQAI
RENGKYKEINDKYFEFDVYGG
>Mature_261_residues
MRITGRIALILSAALVALSASASQAQERMQLKIGTEGAYPPFNNLTADGNLEGFDIDIARALCEEMNAECEFVTQEWDGM
IPALQAGKFDAIIASMSITDERKQQVDFTEKYYNTPPAIVVAKDSDIAGVTKEDLAGRTIGAQASTTHGNFAEATFQESE
VKLYPTAEEYKLDMTNGRLDAVVDDVIVLQDWLSTPDGECCKVLGTLTPVEEIYGEGIGIAIRKGEDELREKFNAAIQAI
RENGKYKEINDKYFEFDVYGG

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=39.7435897435897, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=36.1788617886179, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1787088, Length=249, Percent_Identity=36.144578313253, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1788649, Length=250, Percent_Identity=35.6, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=31.3253012048193, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788228, Length=264, Percent_Identity=29.5454545454545, Blast_Score=121, Evalue=4e-29,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28616; Mature: 28616

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITGRIALILSAALVALSASASQAQERMQLKIGTEGAYPPFNNLTADGNLEGFDIDIAR
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
ALCEEMNAECEFVTQEWDGMIPALQAGKFDAIIASMSITDERKQQVDFTEKYYNTPPAIV
HHHHHCCCCEEEEEHHHCCCCCHHCCCCHHHEEEEECCCHHHHHHCCHHHHHCCCCCEEE
VAKDSDIAGVTKEDLAGRTIGAQASTTHGNFAEATFQESEVKLYPTAEEYKLDMTNGRLD
EECCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECHHHEEEECCCCCHH
AVVDDVIVLQDWLSTPDGECCKVLGTLTPVEEIYGEGIGIAIRKGEDELREKFNAAIQAI
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
RENGKYKEINDKYFEFDVYGG
HHCCCEEECCCCEEEEEECCC
>Mature Secondary Structure
MRITGRIALILSAALVALSASASQAQERMQLKIGTEGAYPPFNNLTADGNLEGFDIDIAR
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
ALCEEMNAECEFVTQEWDGMIPALQAGKFDAIIASMSITDERKQQVDFTEKYYNTPPAIV
HHHHHCCCCEEEEEHHHCCCCCHHCCCCHHHEEEEECCCHHHHHHCCHHHHHCCCCCEEE
VAKDSDIAGVTKEDLAGRTIGAQASTTHGNFAEATFQESEVKLYPTAEEYKLDMTNGRLD
EECCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECHHHEEEECCCCCHH
AVVDDVIVLQDWLSTPDGECCKVLGTLTPVEEIYGEGIGIAIRKGEDELREKFNAAIQAI
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
RENGKYKEINDKYFEFDVYGG
HHCCCEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]