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The map label for this gene is dusA [H]

Identifier: 110633902

GI number: 110633902

Start: 1667616

End: 1668644

Strand: Direct

Name: dusA [H]

Synonym: Meso_1549

Alternate gene names: 110633902

Gene position: 1667616-1668644 (Clockwise)

Preceding gene: 110633899

Following gene: 110633905

Centisome position: 37.79

GC content: 60.64

Gene sequence:

>1029_bases
ATGGGTTCCATGAGCAACGGCGTAGATCGACATCTTCATCACTACGACGGGCGAGTTCTGGCCATCGCTCCAATGATGGA
CTGGACGGATCGCCATTGCCGTTTCTTCCATCGCCAGCTTTCGAAAGATGCGCTCCTCTACACCGAAATGATCGTCGCGG
ACGCGATCATCCACGGCAGCAGGGAACGCCTTCTCGCCTACGATCCGGCGGAGCATCCTGTCGCGTTGCAGCTTGGCGGA
AGCGAGCCGGGAAAACTGGGGGAGGCCGCGCGGATTGCCGCCTCCTTCGGCTATGACGAGATCAATCTCAATGTCGGCTG
TCCGTCGGACCGGGTGCAATCAGGAACCTTCGGCGCATGTCTGATGAAAACGCCTGAACTGGTCTCAGCTTGCGTGGCGG
CCATGAAAGAGTGCGTGGACGTGCCGGTGACGGTGAAATGCCGCATCGGCGTCGACGATCAGGATCCCGAGGAGGCACTG
GACCGGATCGCTGAATTGGTCTTCGCCGCGGGCGCCGATGCCCTTTGGGTGCACGCCAGGAAAGCATGGCTGCAGGGTTT
GAGCCCGCGTGAAAACCGCGAGATTCCTCCGCTCGATTATGACCGGGTTTACCGGCTCAAGAAAAAATATCCGGGCAAGT
TTGTTGGAATCAATGGCGGCATCCAGACCATTGACGAAGCGGTCAATCACCTTGCGCATATGGATGGCGTGATGCTTGGC
CGCGCTGCCTATCATACGCCGGGCATTCTTGCGGAAGTCGATTCCCGCATCTATGGCGGCCAAAATGAGCCCGTCTCCTT
CGCCGAGGTGGTGGAAGCGATGGCGGCCTACGCCCAGCGCCATATTGAGCGGGGAGGGCGCCTTGCCCATGTCACAAGGC
ATATGACTGGCCTGTTCCATGGTCGTGCAGGCGCTCGGCGTTTCCGGCAAATCCTTTCAACGGAGGCGACCCGGCCCAGG
GCAGGCCCGGACGTGCTGCTTCGCGCGCTGGCGGAAATCGATCCGGCCGCACGCCATGAAGCGGCGTAA

Upstream 100 bases:

>100_bases
CAATTTCCCCAACAGTGCCGAGCAGCAGCTGCGGCTTGCTCCAGTCGGCCCTATATGGGAGTAGAAGCCTCATTCGGCGA
ATGCATTTGACCTCCTGAGC

Downstream 100 bases:

>100_bases
GGCGCTGTCCGTGGGCAGCGCCTAGTCAGTCAAGATGAGGCGGTCACCCCGCATTTCGAAGCTCGAGAGGCTGCTCAGGA
ACTGCATCCCAAGCAGGCTT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MGSMSNGVDRHLHHYDGRVLAIAPMMDWTDRHCRFFHRQLSKDALLYTEMIVADAIIHGSRERLLAYDPAEHPVALQLGG
SEPGKLGEAARIAASFGYDEINLNVGCPSDRVQSGTFGACLMKTPELVSACVAAMKECVDVPVTVKCRIGVDDQDPEEAL
DRIAELVFAAGADALWVHARKAWLQGLSPRENREIPPLDYDRVYRLKKKYPGKFVGINGGIQTIDEAVNHLAHMDGVMLG
RAAYHTPGILAEVDSRIYGGQNEPVSFAEVVEAMAAYAQRHIERGGRLAHVTRHMTGLFHGRAGARRFRQILSTEATRPR
AGPDVLLRALAEIDPAARHEAA

Sequences:

>Translated_342_residues
MGSMSNGVDRHLHHYDGRVLAIAPMMDWTDRHCRFFHRQLSKDALLYTEMIVADAIIHGSRERLLAYDPAEHPVALQLGG
SEPGKLGEAARIAASFGYDEINLNVGCPSDRVQSGTFGACLMKTPELVSACVAAMKECVDVPVTVKCRIGVDDQDPEEAL
DRIAELVFAAGADALWVHARKAWLQGLSPRENREIPPLDYDRVYRLKKKYPGKFVGINGGIQTIDEAVNHLAHMDGVMLG
RAAYHTPGILAEVDSRIYGGQNEPVSFAEVVEAMAAYAQRHIERGGRLAHVTRHMTGLFHGRAGARRFRQILSTEATRPR
AGPDVLLRALAEIDPAARHEAA
>Mature_341_residues
GSMSNGVDRHLHHYDGRVLAIAPMMDWTDRHCRFFHRQLSKDALLYTEMIVADAIIHGSRERLLAYDPAEHPVALQLGGS
EPGKLGEAARIAASFGYDEINLNVGCPSDRVQSGTFGACLMKTPELVSACVAAMKECVDVPVTVKCRIGVDDQDPEEALD
RIAELVFAAGADALWVHARKAWLQGLSPRENREIPPLDYDRVYRLKKKYPGKFVGINGGIQTIDEAVNHLAHMDGVMLGR
AAYHTPGILAEVDSRIYGGQNEPVSFAEVVEAMAAYAQRHIERGGRLAHVTRHMTGLFHGRAGARRFRQILSTEATRPRA
GPDVLLRALAEIDPAARHEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=247, Percent_Identity=29.9595141700405, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=55.9748427672956, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789660, Length=307, Percent_Identity=27.3615635179153, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788462, Length=250, Percent_Identity=31.6, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=28.1437125748503, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=296, Percent_Identity=26.3513513513513, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24580595, Length=301, Percent_Identity=26.9102990033223, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920448, Length=301, Percent_Identity=26.9102990033223, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37570; Mature: 37439

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSMSNGVDRHLHHYDGRVLAIAPMMDWTDRHCRFFHRQLSKDALLYTEMIVADAIIHGS
CCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
RERLLAYDPAEHPVALQLGGSEPGKLGEAARIAASFGYDEINLNVGCPSDRVQSGTFGAC
CCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHCCCCCCCHH
LMKTPELVSACVAAMKECVDVPVTVKCRIGVDDQDPEEALDRIAELVFAAGADALWVHAR
HHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
KAWLQGLSPRENREIPPLDYDRVYRLKKKYPGKFVGINGGIQTIDEAVNHLAHMDGVMLG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHC
RAAYHTPGILAEVDSRIYGGQNEPVSFAEVVEAMAAYAQRHIERGGRLAHVTRHMTGLFH
CHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GRAGARRFRQILSTEATRPRAGPDVLLRALAEIDPAARHEAA
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure 
GSMSNGVDRHLHHYDGRVLAIAPMMDWTDRHCRFFHRQLSKDALLYTEMIVADAIIHGS
CCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
RERLLAYDPAEHPVALQLGGSEPGKLGEAARIAASFGYDEINLNVGCPSDRVQSGTFGAC
CCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHCCCCCCCHH
LMKTPELVSACVAAMKECVDVPVTVKCRIGVDDQDPEEALDRIAELVFAAGADALWVHAR
HHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
KAWLQGLSPRENREIPPLDYDRVYRLKKKYPGKFVGINGGIQTIDEAVNHLAHMDGVMLG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHC
RAAYHTPGILAEVDSRIYGGQNEPVSFAEVVEAMAAYAQRHIERGGRLAHVTRHMTGLFH
CHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GRAGARRFRQILSTEATRPRAGPDVLLRALAEIDPAARHEAA
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]