Click here to switch to the map view.

The map label for this gene is pyrC

Identifier: 110633828

GI number: 110633828

Start: 1572618

End: 1573658

Strand: Direct

Name: pyrC

Synonym: Meso_1475

Alternate gene names: 110633828

Gene position: 1572618-1573658 (Clockwise)

Preceding gene: 110633823

Following gene: 110633829

Centisome position: 35.64

GC content: 63.5

Gene sequence:

>1041_bases
ATGAAAACACTTACCATCAGACAGCCGGACGACTGGCACCTACACCTGCGCGATGGCTCCGTGCTTCAGGCGGTGGCGGA
GCACTCGGCGGCTCACTTTGCCCGCGCCATCATCATGCCGAATCTGGTGCCGCCCGTCGTGACCACAAGGGATGCGGCGG
CCTATCGCGAGCGCATCCTGGCGGCGCTGTCTGAAGGCAGCGCCTTTACGCCTCTGATGACGCTCTATCTCACGGAATCG
AGCTGCGCTGACGATATCGAGGCAGGGTTCCGCAGCGGCCTGATCAAGGCGGTCAAGCTCTATCCGGCGGGAGCCACCAC
CAACTCCCAAAGCGGCGTGCGCAACATCGAGGCCGTCTACCCGGTGCTGGAGCGCATGGCGAAAATCGGCCTGCCGCTCT
GCGTGCATGGCGAAGTCACCGATCCCGCGGTCGATATCTTCGACCGAGAGGCGGTGTTCATCGAGCGGGTGCTGCAGCCC
TTGCGCCGCCGCCTGCCGGAACTGCGCATCGTCATGGAGCATGTGACGACGAAGGACGGCGTCGATTTTGCGCTGTCGCA
GGATGAGAATGTCGGCGCGACGATCACGACGCATCACCTGATCATCAACCGCAATGCAATCCTCGCCGGCGGCATTCGCC
CGCATTATTACTGCCTGCCCGTCGCCAAGCGGGAGTCCCATCGCCTCGCGCTGCGCGCGGCCGCCATCTCCGGCGAAGGC
CGCTTCTTCCTCGGCACGGATTCTGCGCCGCATCTGGATCCGCTCAAGGAATGCGCCTGCGGGTGCGCAGGCGTCTTCAA
TGCACCGAACACCATGGCCTGTCTGGCGCATGTGTTCGAGGAGGAGGGCGCGCTCGACCGGCTGGAGGCTTTCACCTCCT
TAAACGGTCCCGCCTTCTACCGCCTGCCGCCGAACGAGAAGCGCATCACGCTCAGGCGTTACGACGAGCCGCTGGAAATG
GAACGGAAAGTCAGCGTGGGGGACGAGGCTATCACCGTCTTCGACCCGATGTTCCCGATCCACTGGAAAGTTGAGGATTA
G

Upstream 100 bases:

>100_bases
AGCATGCCTCATTCCGACAGGAGCCGCGAGGGAATTTCGCTGCGGCGCCGCGAGTTTCGCCTTGCGCTTGCCCGCAAACC
GGATATGTCTCGGCCCTGTC

Downstream 100 bases:

>100_bases
GCTTTTTAGGCGGTCCTTGGCTTGATAGCGAAGAAAGCCGCAAAAAACGCCACTAGCGGGAGCAGGTAATTTGTGGCCGC
GCTGGTGCGAAGCGCCCTAT

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKTLTIRQPDDWHLHLRDGSVLQAVAEHSAAHFARAIIMPNLVPPVVTTRDAAAYRERILAALSEGSAFTPLMTLYLTES
SCADDIEAGFRSGLIKAVKLYPAGATTNSQSGVRNIEAVYPVLERMAKIGLPLCVHGEVTDPAVDIFDREAVFIERVLQP
LRRRLPELRIVMEHVTTKDGVDFALSQDENVGATITTHHLIINRNAILAGGIRPHYYCLPVAKRESHRLALRAAAISGEG
RFFLGTDSAPHLDPLKECACGCAGVFNAPNTMACLAHVFEEEGALDRLEAFTSLNGPAFYRLPPNEKRITLRRYDEPLEM
ERKVSVGDEAITVFDPMFPIHWKVED

Sequences:

>Translated_346_residues
MKTLTIRQPDDWHLHLRDGSVLQAVAEHSAAHFARAIIMPNLVPPVVTTRDAAAYRERILAALSEGSAFTPLMTLYLTES
SCADDIEAGFRSGLIKAVKLYPAGATTNSQSGVRNIEAVYPVLERMAKIGLPLCVHGEVTDPAVDIFDREAVFIERVLQP
LRRRLPELRIVMEHVTTKDGVDFALSQDENVGATITTHHLIINRNAILAGGIRPHYYCLPVAKRESHRLALRAAAISGEG
RFFLGTDSAPHLDPLKECACGCAGVFNAPNTMACLAHVFEEEGALDRLEAFTSLNGPAFYRLPPNEKRITLRRYDEPLEM
ERKVSVGDEAITVFDPMFPIHWKVED
>Mature_346_residues
MKTLTIRQPDDWHLHLRDGSVLQAVAEHSAAHFARAIIMPNLVPPVVTTRDAAAYRERILAALSEGSAFTPLMTLYLTES
SCADDIEAGFRSGLIKAVKLYPAGATTNSQSGVRNIEAVYPVLERMAKIGLPLCVHGEVTDPAVDIFDREAVFIERVLQP
LRRRLPELRIVMEHVTTKDGVDFALSQDENVGATITTHHLIINRNAILAGGIRPHYYCLPVAKRESHRLALRAAAISGEG
RFFLGTDSAPHLDPLKECACGCAGVFNAPNTMACLAHVFEEEGALDRLEAFTSLNGPAFYRLPPNEKRITLRRYDEPLEM
ERKVSVGDEAITVFDPMFPIHWKVED

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=344, Percent_Identity=52.9069767441861, Blast_Score=374, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323452, Length=315, Percent_Identity=35.2380952380952, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_MESSB (Q11IA4)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674036.1
- ProteinModelPortal:   Q11IA4
- SMR:   Q11IA4
- STRING:   Q11IA4
- GeneID:   4179299
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1475
- NMPDR:   fig|266779.1.peg.4361
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q11IA4
- ProtClustDB:   PRK05451
- BioCyc:   MSP266779:MESO_1475-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 38286; Mature: 38286

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTIRQPDDWHLHLRDGSVLQAVAEHSAAHFARAIIMPNLVPPVVTTRDAAAYRERIL
CCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHH
AALSEGSAFTPLMTLYLTESSCADDIEAGFRSGLIKAVKLYPAGATTNSQSGVRNIEAVY
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHH
PVLERMAKIGLPLCVHGEVTDPAVDIFDREAVFIERVLQPLRRRLPELRIVMEHVTTKDG
HHHHHHHHCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCC
VDFALSQDENVGATITTHHLIINRNAILAGGIRPHYYCLPVAKRESHRLALRAAAISGEG
CCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCC
RFFLGTDSAPHLDPLKECACGCAGVFNAPNTMACLAHVFEEEGALDRLEAFTSLNGPAFY
CEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEE
RLPPNEKRITLRRYDEPLEMERKVSVGDEAITVFDPMFPIHWKVED
ECCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEECCCCCEEEEECC
>Mature Secondary Structure
MKTLTIRQPDDWHLHLRDGSVLQAVAEHSAAHFARAIIMPNLVPPVVTTRDAAAYRERIL
CCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHH
AALSEGSAFTPLMTLYLTESSCADDIEAGFRSGLIKAVKLYPAGATTNSQSGVRNIEAVY
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHH
PVLERMAKIGLPLCVHGEVTDPAVDIFDREAVFIERVLQPLRRRLPELRIVMEHVTTKDG
HHHHHHHHCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCC
VDFALSQDENVGATITTHHLIINRNAILAGGIRPHYYCLPVAKRESHRLALRAAAISGEG
CCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCC
RFFLGTDSAPHLDPLKECACGCAGVFNAPNTMACLAHVFEEEGALDRLEAFTSLNGPAFY
CEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEE
RLPPNEKRITLRRYDEPLEMERKVSVGDEAITVFDPMFPIHWKVED
ECCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA