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The map label for this gene is dacA [H]

Identifier: 110633724

GI number: 110633724

Start: 1463333

End: 1464730

Strand: Reverse

Name: dacA [H]

Synonym: Meso_1371

Alternate gene names: 110633724

Gene position: 1464730-1463333 (Counterclockwise)

Preceding gene: 110633728

Following gene: 110633714

Centisome position: 33.2

GC content: 60.44

Gene sequence:

>1398_bases
ATGAAAAGAACCTGCGGACCCCCGCTGGCCGCGGAAGGAACAATTGCTTTGATGGGTGTGGTCGTGCGACAGGCACTGGC
AGAAAAACTACGTAAGGTCACCAATATCGCGGTTCTGGCGGTTGCGACGGGGGCATTTGTTCTGTCACTCGCAGCTTCGG
CTGCGGCAAACCCAAAATACGCCGGTATCGTGATCGATGCGAAAACGGGGCAGGTGCTTTACGAGGACAGTGCCGACGAG
CTGCGGTATCCGGCCTCGCTCACCAAGATGATGACGCTTTACCTCGTATTCGAGTCAATGGCGGCGGGCCGCATCTCCAA
GAGCACGAGCATCACGTTTTCCAAGAATGCGTCCGCTGAACCGCCAACGAAGCTCGGCGTGCCGGCAGGCAAAAGCATTA
CCGTTGAGCAGGCTATCCTGGCGCTCATCACCCGCTCGGCCAATGACGCGGCCACGGCCATTGCGGAAAAGCTGGGCGGG
TCGGAGGAAAGTTTCGCCCGGATGATGACGGCCAAGGCCCGTCAGCTTGGCATGAAATCCACCACCTTCCGCAACGCGCA
TGGCCTTCCCAACAACGCACAGAAAACGACGGCCCGTGACATGGCCCGGCTGGGGATCGCGCTGCGCGAGCACTACCCGC
AATATTATGACTATTTCTCCACCCGCTCATTCGTGTTCTCCGGAAAGAAAATCAACGGCCACAACAATCTTCTTGGCCGC
GTGAAGGGCGCAGACGGCATCAAGACCGGCTATATCAACGCTTCGGGCTTTAATCTCGTCTCCTCGGTCTCGACGGGTGG
ACGGCAGATCGTGGCCGTTGTGATGGGCGGCAAGACGGCGCGCAGCCGCGACGCCCATATGGTGAGTCTCATCGAGCGCT
ATTTGCCCAAGGCATCCACCGGTCGCGGCCAGGACCTGATTGCAAAGGGGCCAGCCATTCGCCCGACAATCACGGATACG
CCCGCGATCATGGCGTCTGCTGCGGCTTTGCCGAAGCGGGCCCCTTTGCCTGCCGAGCGGCCTGACGTCGAAGTTCCCGT
CAACGCCTATGCCGAGGATCCCATTCCGAGCGCCAAGGCGGCACTCGCGGAAGCCGTCGTCGAGCAGAACAACAACTCAG
TCGATCCAGTGGCGACCGCGTCGACCCCTGTCTCCGGCTGGGTGATTCAGGTTGCTTCGGCCCAGACGGAAGAGGATGCG
CGCCGCGTGCTGGAGAAGACGGCAGCGACCGCGTCGGGAATTCTTTCCGGGGTCAAGCCCTTCACTGAAACTTTTGAGCT
CAAAGGGCGTACTTATTATCGCGCTCGCTTCGGCGGCTTCGAAAGCAAGACGGCCGCATGGGATGCCTGTGCGTCGCTGA
AGCGGAAGAGCGTGGATTGTTACGCGGTGCAGAACTAG

Upstream 100 bases:

>100_bases
GGCATCCATAAAACAGGATTTAAAAACGCATCGAATGCGAAAAATCCCGTCAAGCGATCGCTCACCATAACGAATTGGTA
ACGAGGGTTACCTAGGGTCA

Downstream 100 bases:

>100_bases
CCTTCTGCGCCGCCGTTCAAGTAATCGCGTCAGGGAACTAGCGATGATCGGACAGGAGAACGTCCCGAGCCTCGTTGATA
CGAGCGGCAAGAAAGGCGGA

Product: peptidase S11, D-alanyl-D-alanine carboxypeptidase 1

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKRTCGPPLAAEGTIALMGVVVRQALAEKLRKVTNIAVLAVATGAFVLSLAASAAANPKYAGIVIDAKTGQVLYEDSADE
LRYPASLTKMMTLYLVFESMAAGRISKSTSITFSKNASAEPPTKLGVPAGKSITVEQAILALITRSANDAATAIAEKLGG
SEESFARMMTAKARQLGMKSTTFRNAHGLPNNAQKTTARDMARLGIALREHYPQYYDYFSTRSFVFSGKKINGHNNLLGR
VKGADGIKTGYINASGFNLVSSVSTGGRQIVAVVMGGKTARSRDAHMVSLIERYLPKASTGRGQDLIAKGPAIRPTITDT
PAIMASAAALPKRAPLPAERPDVEVPVNAYAEDPIPSAKAALAEAVVEQNNNSVDPVATASTPVSGWVIQVASAQTEEDA
RRVLEKTAATASGILSGVKPFTETFELKGRTYYRARFGGFESKTAAWDACASLKRKSVDCYAVQN

Sequences:

>Translated_465_residues
MKRTCGPPLAAEGTIALMGVVVRQALAEKLRKVTNIAVLAVATGAFVLSLAASAAANPKYAGIVIDAKTGQVLYEDSADE
LRYPASLTKMMTLYLVFESMAAGRISKSTSITFSKNASAEPPTKLGVPAGKSITVEQAILALITRSANDAATAIAEKLGG
SEESFARMMTAKARQLGMKSTTFRNAHGLPNNAQKTTARDMARLGIALREHYPQYYDYFSTRSFVFSGKKINGHNNLLGR
VKGADGIKTGYINASGFNLVSSVSTGGRQIVAVVMGGKTARSRDAHMVSLIERYLPKASTGRGQDLIAKGPAIRPTITDT
PAIMASAAALPKRAPLPAERPDVEVPVNAYAEDPIPSAKAALAEAVVEQNNNSVDPVATASTPVSGWVIQVASAQTEEDA
RRVLEKTAATASGILSGVKPFTETFELKGRTYYRARFGGFESKTAAWDACASLKRKSVDCYAVQN
>Mature_465_residues
MKRTCGPPLAAEGTIALMGVVVRQALAEKLRKVTNIAVLAVATGAFVLSLAASAAANPKYAGIVIDAKTGQVLYEDSADE
LRYPASLTKMMTLYLVFESMAAGRISKSTSITFSKNASAEPPTKLGVPAGKSITVEQAILALITRSANDAATAIAEKLGG
SEESFARMMTAKARQLGMKSTTFRNAHGLPNNAQKTTARDMARLGIALREHYPQYYDYFSTRSFVFSGKKINGHNNLLGR
VKGADGIKTGYINASGFNLVSSVSTGGRQIVAVVMGGKTARSRDAHMVSLIERYLPKASTGRGQDLIAKGPAIRPTITDT
PAIMASAAALPKRAPLPAERPDVEVPVNAYAEDPIPSAKAALAEAVVEQNNNSVDPVATASTPVSGWVIQVASAQTEEDA
RRVLEKTAATASGILSGVKPFTETFELKGRTYYRARFGGFESKTAAWDACASLKRKSVDCYAVQN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=236, Percent_Identity=36.4406779661017, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787062, Length=230, Percent_Identity=37.8260869565217, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082022, Length=264, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87082054, Length=238, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49214; Mature: 49214

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTCGPPLAAEGTIALMGVVVRQALAEKLRKVTNIAVLAVATGAFVLSLAASAAANPKY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AGIVIDAKTGQVLYEDSADELRYPASLTKMMTLYLVFESMAAGRISKSTSITFSKNASAE
EEEEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
PPTKLGVPAGKSITVEQAILALITRSANDAATAIAEKLGGSEESFARMMTAKARQLGMKS
CCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
TTFRNAHGLPNNAQKTTARDMARLGIALREHYPQYYDYFSTRSFVFSGKKINGHNNLLGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCEECCCCCCEEE
VKGADGIKTGYINASGFNLVSSVSTGGRQIVAVVMGGKTARSRDAHMVSLIERYLPKAST
ECCCCCCEECEECCCCCHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCC
GRGQDLIAKGPAIRPTITDTPAIMASAAALPKRAPLPAERPDVEVPVNAYAEDPIPSAKA
CCCCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ALAEAVVEQNNNSVDPVATASTPVSGWVIQVASAQTEEDARRVLEKTAATASGILSGVKP
HHHHHHHHCCCCCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHH
FTETFELKGRTYYRARFGGFESKTAAWDACASLKRKSVDCYAVQN
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MKRTCGPPLAAEGTIALMGVVVRQALAEKLRKVTNIAVLAVATGAFVLSLAASAAANPKY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AGIVIDAKTGQVLYEDSADELRYPASLTKMMTLYLVFESMAAGRISKSTSITFSKNASAE
EEEEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
PPTKLGVPAGKSITVEQAILALITRSANDAATAIAEKLGGSEESFARMMTAKARQLGMKS
CCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
TTFRNAHGLPNNAQKTTARDMARLGIALREHYPQYYDYFSTRSFVFSGKKINGHNNLLGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCEECCCCCCEEE
VKGADGIKTGYINASGFNLVSSVSTGGRQIVAVVMGGKTARSRDAHMVSLIERYLPKAST
ECCCCCCEECEECCCCCHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCC
GRGQDLIAKGPAIRPTITDTPAIMASAAALPKRAPLPAERPDVEVPVNAYAEDPIPSAKA
CCCCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ALAEAVVEQNNNSVDPVATASTPVSGWVIQVASAQTEEDARRVLEKTAATASGILSGVKP
HHHHHHHHCCCCCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHH
FTETFELKGRTYYRARFGGFESKTAAWDACASLKRKSVDCYAVQN
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]