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The map label for this gene is xthA [H]

Identifier: 110633439

GI number: 110633439

Start: 1190586

End: 1191365

Strand: Reverse

Name: xthA [H]

Synonym: Meso_1085

Alternate gene names: 110633439

Gene position: 1191365-1190586 (Counterclockwise)

Preceding gene: 110633440

Following gene: 110633438

Centisome position: 27.0

GC content: 58.33

Gene sequence:

>780_bases
ATGAAGATTGCCACCTTCAACATCAACAATATCAACAAGCGGCTGCCAAACCTTCTCGAATGGCTTCATTTATCAAAGCC
GGACGTGGTTTGCCTGCAGGAATTGAAAGCCACGGACGACGCCTTTCCGAAAAGCGCGATAGAGAGCTTCGGCTATAGAG
CGGTCTGGAAAGGCCAGAAATCCTGGAATGGCGTGGCGATCCTATCGAGGGTAGGCGAGCCTGTTCTGACCAGGACGCGT
CTTCCCGGAGACGCCGCCGACACGCAGGCCCGATATATCGAGGCGGCGGTAAGGGGGGTACTTATCGCTTCGATCTACGC
GCCGAATGGCAATCCGCAGCCGGGACCGAAATTCGACTACAAGCTGGCCTGGTTGGAACGCCTGAGAAAGCATGCGACCG
CGCTCCGGAAAACCGGCGCTCCGATTGTGCTGGCGGGCGATTTTAACGTCGCCCCCACGGATATCGATATTTATCCCACC
AAGTCATGGGGTGATAACGCGCTCATTCAGCCGGAGAGCCGGGCAGCATTCAGGCGGCTTCTCGGCCGAAGCTGGACGGA
TGGCATCCGACTCCTGCATCCGGAACAACGCATATACACGTTCTGGGACTACAAACGCCTGCGCTGGCAGCGCGATGCCG
GGCTGCGGCTCGACCACCTGCTGCTCAGCGAGAATTTACGCGGAGCTATCGTGAAAGCAGGTGTAGACCGCGATGTGAGA
GGCAGGGAAGGCGCGAGCGATCATGCGCCCGCATGGATAGAGCTGAATTTGCGCCGGTGA

Upstream 100 bases:

>100_bases
ACGACCTGAAACCGCGAAAAACTGTCTCCGGATACTCCACCGCCGACCATCTGCGAACTGTCGCCAAGACCGTCTCGGCG
TGGGCAAGACGCATCGCACG

Downstream 100 bases:

>100_bases
CCCTTCGGCTTTGGCATTTAGGGCAGGATGGTGGAGGGGGCTGGATTCGAACCAGCGTAGGCGAAGCCAACGGATTTACA
GTCCGTCTCCTTTAACCACT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKIATFNINNINKRLPNLLEWLHLSKPDVVCLQELKATDDAFPKSAIESFGYRAVWKGQKSWNGVAILSRVGEPVLTRTR
LPGDAADTQARYIEAAVRGVLIASIYAPNGNPQPGPKFDYKLAWLERLRKHATALRKTGAPIVLAGDFNVAPTDIDIYPT
KSWGDNALIQPESRAAFRRLLGRSWTDGIRLLHPEQRIYTFWDYKRLRWQRDAGLRLDHLLLSENLRGAIVKAGVDRDVR
GREGASDHAPAWIELNLRR

Sequences:

>Translated_259_residues
MKIATFNINNINKRLPNLLEWLHLSKPDVVCLQELKATDDAFPKSAIESFGYRAVWKGQKSWNGVAILSRVGEPVLTRTR
LPGDAADTQARYIEAAVRGVLIASIYAPNGNPQPGPKFDYKLAWLERLRKHATALRKTGAPIVLAGDFNVAPTDIDIYPT
KSWGDNALIQPESRAAFRRLLGRSWTDGIRLLHPEQRIYTFWDYKRLRWQRDAGLRLDHLLLSENLRGAIVKAGVDRDVR
GREGASDHAPAWIELNLRR
>Mature_259_residues
MKIATFNINNINKRLPNLLEWLHLSKPDVVCLQELKATDDAFPKSAIESFGYRAVWKGQKSWNGVAILSRVGEPVLTRTR
LPGDAADTQARYIEAAVRGVLIASIYAPNGNPQPGPKFDYKLAWLERLRKHATALRKTGAPIVLAGDFNVAPTDIDIYPT
KSWGDNALIQPESRAAFRRLLGRSWTDGIRLLHPEQRIYTFWDYKRLRWQRDAGLRLDHLLLSENLRGAIVKAGVDRDVR
GREGASDHAPAWIELNLRR

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=29.9242424242424, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=29.9242424242424, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=29.9242424242424, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=33.2075471698113, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71989536, Length=238, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=30.3703703703704, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=30.3703703703704, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29323; Mature: 29323

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATFNINNINKRLPNLLEWLHLSKPDVVCLQELKATDDAFPKSAIESFGYRAVWKGQK
CEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCC
SWNGVAILSRVGEPVLTRTRLPGDAADTQARYIEAAVRGVLIASIYAPNGNPQPGPKFDY
CCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCH
KLAWLERLRKHATALRKTGAPIVLAGDFNVAPTDIDIYPTKSWGDNALIQPESRAAFRRL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCEECCHHHHHHHHH
LGRSWTDGIRLLHPEQRIYTFWDYKRLRWQRDAGLRLDHLLLSENLRGAIVKAGVDRDVR
HCCCCCCCEEEECCCHHEEEEEHHHHHHHHCCCCCCHHHHHHHCCCCCEEEECCCCCCCC
GREGASDHAPAWIELNLRR
CCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKIATFNINNINKRLPNLLEWLHLSKPDVVCLQELKATDDAFPKSAIESFGYRAVWKGQK
CEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCC
SWNGVAILSRVGEPVLTRTRLPGDAADTQARYIEAAVRGVLIASIYAPNGNPQPGPKFDY
CCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCH
KLAWLERLRKHATALRKTGAPIVLAGDFNVAPTDIDIYPTKSWGDNALIQPESRAAFRRL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCEECCHHHHHHHHH
LGRSWTDGIRLLHPEQRIYTFWDYKRLRWQRDAGLRLDHLLLSENLRGAIVKAGVDRDVR
HCCCCCCCEEEECCCHHEEEEEHHHHHHHHCCCCCCHHHHHHHCCCCCEEEECCCCCCCC
GREGASDHAPAWIELNLRR
CCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]