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The map label for this gene is rlmB [H]

Identifier: 110633438

GI number: 110633438

Start: 1189438

End: 1190268

Strand: Reverse

Name: rlmB [H]

Synonym: Meso_1084

Alternate gene names: 110633438

Gene position: 1190268-1189438 (Counterclockwise)

Preceding gene: 110633439

Following gene: 110633436

Centisome position: 26.98

GC content: 63.18

Gene sequence:

>831_bases
ATGAGCGACCATAAGAAGACAGGAACGCCCAAGGATAGCCACTATGCGCGGCTACGCAGGGCTCATCGCGAACGCACCGC
GCCCAATGAACGCGCGGGAGCGGATTTACCGGAAGGAATCGTGCGCCTCTATGGCTTGCATACCGTGCGCGCGGCCATCG
ACAATCCTGCTCGCTCGATCCGTCAAATGCTGGTGACGCGCAACGCAGCTCAAAGGCTGGGGATTGCCGATCCCAGCACC
CTGCCCTTTCCGGTGGAAATTGTGGAACCCAAGGCCATCGACGCATTGGTGGGGGCCGATGCGGTGCATCAGGGCGTGCT
CGTGGAAGCCACGGCCCGCACCCCAAAGCCATTCTCCGGCCTTGAGGATGCCTCGCTGGTCCTGGTTCTCGATCAGGTGA
CCGATCCGCACAATGTCGGTGCGATCATGCGTTCGGCGGTGGCCTTCGGTGCCGATGCGCTGGTGACCACCGCGCGGCAC
AGCCCCGCCGAATCCGGCGTTCTCGCCAAGGCCGCATCCGGCGCGTTGGAGCATATTGATCATATCGAGGTGCGCAACCT
CGCCAACGCGATCGAGGAGTTGCATGCGGCCGGCTTCCTCACGGTCGGGCTCGACTCGGAAGGCCCGCAGCGCTTGGAAA
AGACGCTGCAGGGCAAGAAGATTGCCCTCGTGTTGGGATCAGAGGGCAAGGGCCTGCGTCAGAAAACGCGCGAAACGGTC
CGGGCGCTCGCGCGCCTTGACATGCCGGGAGCCATTCATTCCCTCAATGTTTCGAACGCGGCCGCTGTCGCTCTCTATAT
AGCGCGCCGATCGCTTGCCGAGGGCTCATAG

Upstream 100 bases:

>100_bases
CCGCCTGTCAACAGCGTCAAAGCGCATCACAGCGGAAAATCGCAAGAACCCGCCCCTGCGGCCATAGGCGTTCGGCCTCC
TCTCGTTTATAGGAGGCGCC

Downstream 100 bases:

>100_bases
ACCAAACCACCAGGTGGCAAGGCCGAGGAAGCCGAAAAAGCCTACCACATCCGTTATCGCCGTCACGAAAACGGATGAAG
CCACCGCGGGGTCCGCGCCA

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSDHKKTGTPKDSHYARLRRAHRERTAPNERAGADLPEGIVRLYGLHTVRAAIDNPARSIRQMLVTRNAAQRLGIADPST
LPFPVEIVEPKAIDALVGADAVHQGVLVEATARTPKPFSGLEDASLVLVLDQVTDPHNVGAIMRSAVAFGADALVTTARH
SPAESGVLAKAASGALEHIDHIEVRNLANAIEELHAAGFLTVGLDSEGPQRLEKTLQGKKIALVLGSEGKGLRQKTRETV
RALARLDMPGAIHSLNVSNAAAVALYIARRSLAEGS

Sequences:

>Translated_276_residues
MSDHKKTGTPKDSHYARLRRAHRERTAPNERAGADLPEGIVRLYGLHTVRAAIDNPARSIRQMLVTRNAAQRLGIADPST
LPFPVEIVEPKAIDALVGADAVHQGVLVEATARTPKPFSGLEDASLVLVLDQVTDPHNVGAIMRSAVAFGADALVTTARH
SPAESGVLAKAASGALEHIDHIEVRNLANAIEELHAAGFLTVGLDSEGPQRLEKTLQGKKIALVLGSEGKGLRQKTRETV
RALARLDMPGAIHSLNVSNAAAVALYIARRSLAEGS
>Mature_275_residues
SDHKKTGTPKDSHYARLRRAHRERTAPNERAGADLPEGIVRLYGLHTVRAAIDNPARSIRQMLVTRNAAQRLGIADPSTL
PFPVEIVEPKAIDALVGADAVHQGVLVEATARTPKPFSGLEDASLVLVLDQVTDPHNVGAIMRSAVAFGADALVTTARHS
PAESGVLAKAASGALEHIDHIEVRNLANAIEELHAAGFLTVGLDSEGPQRLEKTLQGKKIALVLGSEGKGLRQKTRETVR
ALARLDMPGAIHSLNVSNAAAVALYIARRSLAEGS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=255, Percent_Identity=30.5882352941176, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1790623, Length=245, Percent_Identity=30.2040816326531, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788935, Length=140, Percent_Identity=34.2857142857143, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17555908, Length=245, Percent_Identity=27.7551020408163, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29319; Mature: 29188

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHKKTGTPKDSHYARLRRAHRERTAPNERAGADLPEGIVRLYGLHTVRAAIDNPARSI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
RQMLVTRNAAQRLGIADPSTLPFPVEIVEPKAIDALVGADAVHQGVLVEATARTPKPFSG
HHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
LEDASLVLVLDQVTDPHNVGAIMRSAVAFGADALVTTARHSPAESGVLAKAASGALEHID
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
HIEVRNLANAIEELHAAGFLTVGLDSEGPQRLEKTLQGKKIALVLGSEGKGLRQKTRETV
HHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
RALARLDMPGAIHSLNVSNAAAVALYIARRSLAEGS
HHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDHKKTGTPKDSHYARLRRAHRERTAPNERAGADLPEGIVRLYGLHTVRAAIDNPARSI
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
RQMLVTRNAAQRLGIADPSTLPFPVEIVEPKAIDALVGADAVHQGVLVEATARTPKPFSG
HHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
LEDASLVLVLDQVTDPHNVGAIMRSAVAFGADALVTTARHSPAESGVLAKAASGALEHID
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
HIEVRNLANAIEELHAAGFLTVGLDSEGPQRLEKTLQGKKIALVLGSEGKGLRQKTRETV
HHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
RALARLDMPGAIHSLNVSNAAAVALYIARRSLAEGS
HHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]