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The map label for this gene is garL [H]

Identifier: 110633411

GI number: 110633411

Start: 1160821

End: 1161594

Strand: Reverse

Name: garL [H]

Synonym: Meso_1057

Alternate gene names: 110633411

Gene position: 1161594-1160821 (Counterclockwise)

Preceding gene: 110633412

Following gene: 110633410

Centisome position: 26.33

GC content: 60.98

Gene sequence:

>774_bases
ATGGCGAACCTCGCTCTGGAAAGACTAAGACGGCAGGAGCTGTCCCTCGGCGTCGTCCTGCGCCAGGCGCGTAGCGTGGA
AACGGGAAAAATCTTTCAAACAGCGGGTTTCGACTGGCTGTTCCTCGATCTTGAGCACAACTCGATGTCGATCGAAACGG
CCTGCGAAATCTCCGTCGCTTGCCTCGGGACAGGAATAGCGCCGCTGGTTCGGGTACCGGAAAACCAGTACTGGATGGCA
ACGCGGGCGCTGGACGGCGGTGCGCAGGGCATCGTGTTGCCGCATGTGGATACGGAGGAGCAGGCGCGCGAGACCGTCAG
GCAACTCCGCTATCCGCCGGCCGGCAGGCGCTCCATTTCCTCCATGCAGGTGCATGTCGATTTCCGCTCCGTGCCGGTGG
GCGAGCTTACTGCCCAACTCGACAAGGATTTTCTGATCGCCGTCATGCTCGAAACCATCACGGCGATTGAGAATAGCGAG
GCAATCGCGGCTGTGGACGGCATCGATGTCGTCATGATCGGCGCGAACGATCTCTGCCTCGACATGGGTATAGCGGGCGA
CGTCATGAACGAGCGCGTCATAAAGGCGTTCGAACGCGTCGCGGCAGCCTGCCGGAAGCACGGTCGTTATGCCGGGCTCG
GCGGGGTGCGCAGACCGGAAGACCTCAAGGTCTATTTTGACATGGGATACCGTTTCATCCTTGCGGCAAACGACGTCACC
ATGCTGGTCGATGCCGGCCAGCGGCGGACGGCATCGCTTCGCAGTCTCATCTGA

Upstream 100 bases:

>100_bases
CGCTGCCCAAATCCGATCTCGTTGTGGTCCCCGACAAGCCGCGCCAGTTCGCCGGTGCGGGCCCGGTGGCGGTGTAATCC
CAAACGAGAGGCATTCCACG

Downstream 100 bases:

>100_bases
AGCCGGGATCCGGAGGGCAGGTCATGGGGGTAATCCTTGGTAGCGGCGACTTCACCTATCGGGTCGAGGAGCAATGGGTG
AAGCTTCCACCCGGCATGGT

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MANLALERLRRQELSLGVVLRQARSVETGKIFQTAGFDWLFLDLEHNSMSIETACEISVACLGTGIAPLVRVPENQYWMA
TRALDGGAQGIVLPHVDTEEQARETVRQLRYPPAGRRSISSMQVHVDFRSVPVGELTAQLDKDFLIAVMLETITAIENSE
AIAAVDGIDVVMIGANDLCLDMGIAGDVMNERVIKAFERVAAACRKHGRYAGLGGVRRPEDLKVYFDMGYRFILAANDVT
MLVDAGQRRTASLRSLI

Sequences:

>Translated_257_residues
MANLALERLRRQELSLGVVLRQARSVETGKIFQTAGFDWLFLDLEHNSMSIETACEISVACLGTGIAPLVRVPENQYWMA
TRALDGGAQGIVLPHVDTEEQARETVRQLRYPPAGRRSISSMQVHVDFRSVPVGELTAQLDKDFLIAVMLETITAIENSE
AIAAVDGIDVVMIGANDLCLDMGIAGDVMNERVIKAFERVAAACRKHGRYAGLGGVRRPEDLKVYFDMGYRFILAANDVT
MLVDAGQRRTASLRSLI
>Mature_256_residues
ANLALERLRRQELSLGVVLRQARSVETGKIFQTAGFDWLFLDLEHNSMSIETACEISVACLGTGIAPLVRVPENQYWMAT
RALDGGAQGIVLPHVDTEEQARETVRQLRYPPAGRRSISSMQVHVDFRSVPVGELTAQLDKDFLIAVMLETITAIENSEA
IAAVDGIDVVMIGANDLCLDMGIAGDVMNERVIKAFERVAAACRKHGRYAGLGGVRRPEDLKVYFDMGYRFILAANDVTM
LVDAGQRRTASLRSLI

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788578, Length=255, Percent_Identity=30.5882352941176, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 28279; Mature: 28148

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLALERLRRQELSLGVVLRQARSVETGKIFQTAGFDWLFLDLEHNSMSIETACEISVA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEECCCEEEEHHHEEEEE
CLGTGIAPLVRVPENQYWMATRALDGGAQGIVLPHVDTEEQARETVRQLRYPPAGRRSIS
ECCCCCHHEEECCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
SMQVHVDFRSVPVGELTAQLDKDFLIAVMLETITAIENSEAIAAVDGIDVVMIGANDLCL
EEEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCEEE
DMGIAGDVMNERVIKAFERVAAACRKHGRYAGLGGVRRPEDLKVYFDMGYRFILAANDVT
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCEEEEEEECCEE
MLVDAGQRRTASLRSLI
EEEECCCCHHHHHHHCC
>Mature Secondary Structure 
ANLALERLRRQELSLGVVLRQARSVETGKIFQTAGFDWLFLDLEHNSMSIETACEISVA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEECCCEEEEHHHEEEEE
CLGTGIAPLVRVPENQYWMATRALDGGAQGIVLPHVDTEEQARETVRQLRYPPAGRRSIS
ECCCCCHHEEECCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
SMQVHVDFRSVPVGELTAQLDKDFLIAVMLETITAIENSEAIAAVDGIDVVMIGANDLCL
EEEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCEEE
DMGIAGDVMNERVIKAFERVAAACRKHGRYAGLGGVRRPEDLKVYFDMGYRFILAANDVT
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCEEEEEEECCEE
MLVDAGQRRTASLRSLI
EEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA