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The map label for this gene is purF [H]

Identifier: 110633337

GI number: 110633337

Start: 1078211

End: 1079683

Strand: Reverse

Name: purF [H]

Synonym: Meso_0983

Alternate gene names: 110633337

Gene position: 1079683-1078211 (Counterclockwise)

Preceding gene: 110633338

Following gene: 110633335

Centisome position: 24.47

GC content: 60.83

Gene sequence:

>1473_bases
ATGGCGGAACGTGAGAATTTGCCTCTGATGGGCGAAGCCGACGATCATTTCCACGACGAATGCGGGGTGTTCGGCATTTT
CGGGCGCCAGGATGCGGCTGCTATCGTCACCCTGGGACTGCATGCCCTCCAGCATCGCGGCCAGGAAGCGGCAGGTATCG
TGTCATTCGACGGCAGCCAATTCCATGTCGAACGCCATATCGGCCTGATCGGCGATACGTTCACAAAGCCTGCGGTCCTT
GAGCGCCTCAAGGGCTCGCGTGCAATCGGCCATACCCGTTATTCCACCACCGGCGGCTCGGGCCTGCGCAATGTGCAGCC
CTTCTTTGCCGAGCTTTCCGACGGCGGGCTGGCGCTCGCCCATAACGGCAATATCACCAACGCGATGACTGTGCAGCGCC
GGCTGCAAAAGCAGGGCGCAATTTTCTCCTCCACCTCCGACACCGAAACGATCCTCCACCTGGTGGCGAGCAGCAAGGAG
AACAATCTCAACACACGGTTCATCGATGCGGTGCGGCAGCTGGAAGGCGCTTTCTCGCTCGTGGCGCTCACATCCAAAAA
GATGATCGGGTGCCGAGATCCGCTGGGCATCCGCCCGCTGGTGCTGGGTGATCTCGACGGCGCCTATATACTGGCTTCCG
AGACCTGTGCGCTTGACATTATCGGCGCACGTTTCGTCCGCGACCTCAATGCCGGCGAGATGGTAGTCATCACCGAGCAC
GGCATTGAAAGCCACTTTCCCTTTGAACCCGTACGCCAGCGGTTTTGCATCTTCGAATATGTCTATTTCGCCAGGCCGGA
TTCCTCCGTCGAAGGGCGCAATGTCTACGAGGTGCGCAAGCGCATTGGCGCGGAGCTCGCGCGGGAAAACCCGGTCGAGG
CCGATATCGTCATTCCGGTGCCCGATTCCGGCACGCCTGCCGCTATCGGCTTCTCGCAGGAAGCTGGAATTCCCTTCGAG
CTCGGCATCATCCGCAACCATTATGTCGGCCGCACCTTCATCCAGCCGACGGATGCCATCCGCCACATGGGCGTGAAGCT
CAAGCACAATGCAAACCGGCGTTCCATCGAAGGCAAGCGCGTCGTGCTGGTCGACGATTCGATCGTCCGCGGCACGACGA
GCCAGAAAATTGTGCAGATGGTCCGCGAGGCGGGCGCGAGGGAAGTCCATATGCGCATTGCCTCACCGCCGACACGCGCT
TCGTGCTTTTACGGGGTCGATACGCCACAAACGGCGAAGCTGCTGGCCTCGCGCATGTCCGTCGAGGAGATGGCCGACTT
CATTCGCGTCGATTCTCTGGGTTTTCTATCGATCGACGGGCTCTACAGGGCGGTCGGTGAAGCGGCGCGTAACGGTGAGG
CACCGCAATTCTGCGATGCCTGCTTCACCGCCGAATATCCGACCCAGCTTACCGACCACCATGGTACCGACAATGTACGC
CAGCTGTCGCTGCTCGCGGCGGGCGGGCAATAG

Upstream 100 bases:

>100_bases
ACGGGATCGCCTCAACAGGGAGCGGCGGACGGAGCTGAAACAAGCAACTGACGGTCTGTGATGGCCGCCGAGCACACCAA
CTGAAACGAGGCGGCCAGTG

Downstream 100 bases:

>100_bases
TGACATTCAAGGTGTGACGAGCTTCAGGCCGATAATGCCGGCCACGATCAGGCCGATGCAGGCGAGCCGGATGGCCTCGG
CCGACTCGCCGAAAAGCATA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MAERENLPLMGEADDHFHDECGVFGIFGRQDAAAIVTLGLHALQHRGQEAAGIVSFDGSQFHVERHIGLIGDTFTKPAVL
ERLKGSRAIGHTRYSTTGGSGLRNVQPFFAELSDGGLALAHNGNITNAMTVQRRLQKQGAIFSSTSDTETILHLVASSKE
NNLNTRFIDAVRQLEGAFSLVALTSKKMIGCRDPLGIRPLVLGDLDGAYILASETCALDIIGARFVRDLNAGEMVVITEH
GIESHFPFEPVRQRFCIFEYVYFARPDSSVEGRNVYEVRKRIGAELARENPVEADIVIPVPDSGTPAAIGFSQEAGIPFE
LGIIRNHYVGRTFIQPTDAIRHMGVKLKHNANRRSIEGKRVVLVDDSIVRGTTSQKIVQMVREAGAREVHMRIASPPTRA
SCFYGVDTPQTAKLLASRMSVEEMADFIRVDSLGFLSIDGLYRAVGEAARNGEAPQFCDACFTAEYPTQLTDHHGTDNVR
QLSLLAAGGQ

Sequences:

>Translated_490_residues
MAERENLPLMGEADDHFHDECGVFGIFGRQDAAAIVTLGLHALQHRGQEAAGIVSFDGSQFHVERHIGLIGDTFTKPAVL
ERLKGSRAIGHTRYSTTGGSGLRNVQPFFAELSDGGLALAHNGNITNAMTVQRRLQKQGAIFSSTSDTETILHLVASSKE
NNLNTRFIDAVRQLEGAFSLVALTSKKMIGCRDPLGIRPLVLGDLDGAYILASETCALDIIGARFVRDLNAGEMVVITEH
GIESHFPFEPVRQRFCIFEYVYFARPDSSVEGRNVYEVRKRIGAELARENPVEADIVIPVPDSGTPAAIGFSQEAGIPFE
LGIIRNHYVGRTFIQPTDAIRHMGVKLKHNANRRSIEGKRVVLVDDSIVRGTTSQKIVQMVREAGAREVHMRIASPPTRA
SCFYGVDTPQTAKLLASRMSVEEMADFIRVDSLGFLSIDGLYRAVGEAARNGEAPQFCDACFTAEYPTQLTDHHGTDNVR
QLSLLAAGGQ
>Mature_489_residues
AERENLPLMGEADDHFHDECGVFGIFGRQDAAAIVTLGLHALQHRGQEAAGIVSFDGSQFHVERHIGLIGDTFTKPAVLE
RLKGSRAIGHTRYSTTGGSGLRNVQPFFAELSDGGLALAHNGNITNAMTVQRRLQKQGAIFSSTSDTETILHLVASSKEN
NLNTRFIDAVRQLEGAFSLVALTSKKMIGCRDPLGIRPLVLGDLDGAYILASETCALDIIGARFVRDLNAGEMVVITEHG
IESHFPFEPVRQRFCIFEYVYFARPDSSVEGRNVYEVRKRIGAELARENPVEADIVIPVPDSGTPAAIGFSQEAGIPFEL
GIIRNHYVGRTFIQPTDAIRHMGVKLKHNANRRSIEGKRVVLVDDSIVRGTTSQKIVQMVREAGAREVHMRIASPPTRAS
CFYGVDTPQTAKLLASRMSVEEMADFIRVDSLGFLSIDGLYRAVGEAARNGEAPQFCDACFTAEYPTQLTDHHGTDNVRQ
LSLLAAGGQ

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=508, Percent_Identity=37.992125984252, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI4826742, Length=188, Percent_Identity=31.9148936170213, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788651, Length=477, Percent_Identity=39.2033542976939, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1790167, Length=229, Percent_Identity=30.1310043668122, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=39.0396659707724, Blast_Score=328, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=34.9896480331263, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=40.9836065573771, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=492, Percent_Identity=41.4634146341463, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=41.3276231263383, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI21357745, Length=207, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53513; Mature: 53382

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERENLPLMGEADDHFHDECGVFGIFGRQDAAAIVTLGLHALQHRGQEAAGIVSFDGSQ
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCE
FHVERHIGLIGDTFTKPAVLERLKGSRAIGHTRYSTTGGSGLRNVQPFFAELSDGGLALA
EEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEECCCCCCCCCCCCHHHHHCCCCCEEEE
HNGNITNAMTVQRRLQKQGAIFSSTSDTETILHLVASSKENNLNTRFIDAVRQLEGAFSL
ECCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEE
VALTSKKMIGCRDPLGIRPLVLGDLDGAYILASETCALDIIGARFVRDLNAGEMVVITEH
EEECCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEHHHHHHHHHCCCCCCEEEEEEC
GIESHFPFEPVRQRFCIFEYVYFARPDSSVEGRNVYEVRKRIGAELARENPVEADIVIPV
CCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDSGTPAAIGFSQEAGIPFELGIIRNHYVGRTFIQPTDAIRHMGVKLKHNANRRSIEGKR
CCCCCCCEEECCCCCCCCEEEEEECCCCCCCEECCHHHHHHHCCEEEEECCCCCCCCCCE
VVLVDDSIVRGTTSQKIVQMVREAGAREVHMRIASPPTRASCFYGVDTPQTAKLLASRMS
EEEECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHCC
VEEMADFIRVDSLGFLSIDGLYRAVGEAARNGEAPQFCDACFTAEYPTQLTDHHGTDNVR
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHCCCCCCCCCE
QLSLLAAGGQ
EEEEEECCCC
>Mature Secondary Structure 
AERENLPLMGEADDHFHDECGVFGIFGRQDAAAIVTLGLHALQHRGQEAAGIVSFDGSQ
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCE
FHVERHIGLIGDTFTKPAVLERLKGSRAIGHTRYSTTGGSGLRNVQPFFAELSDGGLALA
EEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEECCCCCCCCCCCCHHHHHCCCCCEEEE
HNGNITNAMTVQRRLQKQGAIFSSTSDTETILHLVASSKENNLNTRFIDAVRQLEGAFSL
ECCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEE
VALTSKKMIGCRDPLGIRPLVLGDLDGAYILASETCALDIIGARFVRDLNAGEMVVITEH
EEECCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEHHHHHHHHHCCCCCCEEEEEEC
GIESHFPFEPVRQRFCIFEYVYFARPDSSVEGRNVYEVRKRIGAELARENPVEADIVIPV
CCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDSGTPAAIGFSQEAGIPFELGIIRNHYVGRTFIQPTDAIRHMGVKLKHNANRRSIEGKR
CCCCCCCEEECCCCCCCCEEEEEECCCCCCCEECCHHHHHHHCCEEEEECCCCCCCCCCE
VVLVDDSIVRGTTSQKIVQMVREAGAREVHMRIASPPTRASCFYGVDTPQTAKLLASRMS
EEEECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHCC
VEEMADFIRVDSLGFLSIDGLYRAVGEAARNGEAPQFCDACFTAEYPTQLTDHHGTDNVR
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHCCCCCCCCCE
QLSLLAAGGQ
EEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA