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The map label for this gene is smeIM [H]

Identifier: 110633120

GI number: 110633120

Start: 837932

End: 839050

Strand: Direct

Name: smeIM [H]

Synonym: Meso_0763

Alternate gene names: 110633120

Gene position: 837932-839050 (Clockwise)

Preceding gene: 110633118

Following gene: 110633123

Centisome position: 18.99

GC content: 63.99

Gene sequence:

>1119_bases
ATGTCCGCTGTGCGTCCGCTTGAGGAGCTTTCCCCGCTTCCCGCCAAGGCCGATTGGCTGGACGATATCCTGAAAGGCGA
CTGTGTCGCCGCTCTTGAGCGGCTTCCCGAAAAGTCGGTGGACGTGATCTTCGCCGATCCGCCCTACAACCTCCAGCTCG
AAGGGGCGCTCCATCGTCCCGACCAGTCCCTGGTCGATGCGGTCGACGACGAGTGGGACCAGTTCGAAAGCTTCGCCGCC
TATGACGCTTTCACGCGAGCATGGCTTCTGGCGGCCCGCCGCGTGCTTAAGCCGAACGGAACCGTCTGGGTCATCGGCTC
CTACCACAACATCTTCCGGGTTGGCGCGATCATGCAGGATCTGGGCTTCTGGATCCTGAACGACATCGTCTGGCGCAAGA
CCAATCCGATGCCGAATTTCCGCGGCCGCCGCTTCCAGAACGCCCATGAGACGATGATCTGGGCCTCGCGCGACCGCAAC
GCGAAGGGCTACACCTTCAATTACGAGGCCATGAAAGCCGCCAATGACGACCTGCAGATGCGGTCGGACTGGCTGTTTCC
GATCTGCACCGGCAAGGAGCGGCTCAAAGACGCTGACGGTAACAAGGTCCATCCGACGCAGAAGCCGGAAGCGCTGCTTG
CGCGCGTGCTCCTGTCTTCAACGAAGCCAGGCGATGTGGTCCTCGACCCATTCTTCGGCTCAGGCACCACCGGGGCGGTT
GCCAAGCGCCTTGGCCGCCACTTTGTCGGCATCGAGCGGGACCCGGATTATATCGCAGCGGCCCGAGAGCGCATTGCGGC
GGTGGAGCCTTTGGATCAAGCCGCGCTCGCGCCGATGGCGGCAAAACGTGAGGAGCCGCGCATCGCCTTTGCGAGCCTGC
TGGACTCTGGCCTCGTGAAGCCCGGTATGCAGCTTTACGACAGCAAAAGGCGCTGGGCGGCGCATGTGCGGGCCGACGGC
ACGCTCGCAGTTGGCCCGGAGACGGGCTCGATCCACCGGCTCGGCGCCCGCGTCCAGGGCCTGGACGCCTGCAACGGCTG
GACCTTTTGGCATTATGAGGAAAAAGGGGCGCTCAAGCCCATCGACGAATTGCGGCAGGTGGCGCGCCGCGGCCTGTAG

Upstream 100 bases:

>100_bases
TCTACTCTCATAAGAGCGAATTGGTTCCCCGTCTTACGGAAATATTTACCCTCCTCGGTAACCATAACGGCCGGTAAAAA
GTTGCGTCGAGGTTTTGCGT

Downstream 100 bases:

>100_bases
GGCTGGGGGTGTAACCGGCCCCCCCTCTTGCGTTTTCCAAGCACTTGCTCCGGGACGAGCCCGCTCGCAATTGCAGGAAA
ACGCTTTCTCCCCCACCAAG

Product: DNA adenine methylase CcrM

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSAVRPLEELSPLPAKADWLDDILKGDCVAALERLPEKSVDVIFADPPYNLQLEGALHRPDQSLVDAVDDEWDQFESFAA
YDAFTRAWLLAARRVLKPNGTVWVIGSYHNIFRVGAIMQDLGFWILNDIVWRKTNPMPNFRGRRFQNAHETMIWASRDRN
AKGYTFNYEAMKAANDDLQMRSDWLFPICTGKERLKDADGNKVHPTQKPEALLARVLLSSTKPGDVVLDPFFGSGTTGAV
AKRLGRHFVGIERDPDYIAAARERIAAVEPLDQAALAPMAAKREEPRIAFASLLDSGLVKPGMQLYDSKRRWAAHVRADG
TLAVGPETGSIHRLGARVQGLDACNGWTFWHYEEKGALKPIDELRQVARRGL

Sequences:

>Translated_372_residues
MSAVRPLEELSPLPAKADWLDDILKGDCVAALERLPEKSVDVIFADPPYNLQLEGALHRPDQSLVDAVDDEWDQFESFAA
YDAFTRAWLLAARRVLKPNGTVWVIGSYHNIFRVGAIMQDLGFWILNDIVWRKTNPMPNFRGRRFQNAHETMIWASRDRN
AKGYTFNYEAMKAANDDLQMRSDWLFPICTGKERLKDADGNKVHPTQKPEALLARVLLSSTKPGDVVLDPFFGSGTTGAV
AKRLGRHFVGIERDPDYIAAARERIAAVEPLDQAALAPMAAKREEPRIAFASLLDSGLVKPGMQLYDSKRRWAAHVRADG
TLAVGPETGSIHRLGARVQGLDACNGWTFWHYEEKGALKPIDELRQVARRGL
>Mature_371_residues
SAVRPLEELSPLPAKADWLDDILKGDCVAALERLPEKSVDVIFADPPYNLQLEGALHRPDQSLVDAVDDEWDQFESFAAY
DAFTRAWLLAARRVLKPNGTVWVIGSYHNIFRVGAIMQDLGFWILNDIVWRKTNPMPNFRGRRFQNAHETMIWASRDRNA
KGYTFNYEAMKAANDDLQMRSDWLFPICTGKERLKDADGNKVHPTQKPEALLARVLLSSTKPGDVVLDPFFGSGTTGAVA
KRLGRHFVGIERDPDYIAAARERIAAVEPLDQAALAPMAAKREEPRIAFASLLDSGLVKPGMQLYDSKRRWAAHVRADGT
LAVGPETGSIHRLGARVQGLDACNGWTFWHYEEKGALKPIDELRQVARRGL

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=285, Percent_Identity=32.280701754386, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 41673; Mature: 41542

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVRPLEELSPLPAKADWLDDILKGDCVAALERLPEKSVDVIFADPPYNLQLEGALHRP
CCCCCCHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCCEEECCCCCCC
DQSLVDAVDDEWDQFESFAAYDAFTRAWLLAARRVLKPNGTVWVIGSYHNIFRVGAIMQD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
LGFWILNDIVWRKTNPMPNFRGRRFQNAHETMIWASRDRNAKGYTFNYEAMKAANDDLQM
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEECCCCCCCCEEEEHHHHHHCCCCHHH
RSDWLFPICTGKERLKDADGNKVHPTQKPEALLARVLLSSTKPGDVVLDPFFGSGTTGAV
HCCCCEEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHH
AKRLGRHFVGIERDPDYIAAARERIAAVEPLDQAALAPMAAKREEPRIAFASLLDSGLVK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
PGMQLYDSKRRWAAHVRADGTLAVGPETGSIHRLGARVQGLDACNGWTFWHYEEKGALKP
CCHHHHHHHHHHHHHEECCCEEEECCCCCCHHHHCCHHCCCCCCCCCEEEEECCCCCCCC
IDELRQVARRGL
HHHHHHHHHHCC
>Mature Secondary Structure 
SAVRPLEELSPLPAKADWLDDILKGDCVAALERLPEKSVDVIFADPPYNLQLEGALHRP
CCCCCHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCCEEECCCCCCC
DQSLVDAVDDEWDQFESFAAYDAFTRAWLLAARRVLKPNGTVWVIGSYHNIFRVGAIMQD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
LGFWILNDIVWRKTNPMPNFRGRRFQNAHETMIWASRDRNAKGYTFNYEAMKAANDDLQM
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEECCCCCCCCEEEEHHHHHHCCCCHHH
RSDWLFPICTGKERLKDADGNKVHPTQKPEALLARVLLSSTKPGDVVLDPFFGSGTTGAV
HCCCCEEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHH
AKRLGRHFVGIERDPDYIAAARERIAAVEPLDQAALAPMAAKREEPRIAFASLLDSGLVK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
PGMQLYDSKRRWAAHVRADGTLAVGPETGSIHRLGARVQGLDACNGWTFWHYEEKGALKP
CCHHHHHHHHHHHHHEECCCEEEECCCCCCHHHHCCHHCCCCCCCCCEEEEECCCCCCCC
IDELRQVARRGL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]