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The map label for this gene is rlmE

Identifier: 110632831

GI number: 110632831

Start: 518819

End: 519526

Strand: Reverse

Name: rlmE

Synonym: Meso_0470

Alternate gene names: 110632831

Gene position: 519526-518819 (Counterclockwise)

Preceding gene: 110632832

Following gene: 110632829

Centisome position: 11.77

GC content: 62.57

Gene sequence:

>708_bases
GTGACGAAGGAGAAGAAGGCTGGCTCCGGGACGCGCGTCCTGAAAACGCGGGTCAAGAAGAAGCGGGGCTTGAAGAGCTC
GTCCCGGCGCTGGCTGGAGCGGCATCTGAACGATCCTTACGTGCGCCGAGCCAATTCGGAGGGAATGCGCTCGCGCGCCG
CCTACAAGCTGATCGAGATCGACGATAAGCACCACATACTGGCACCAGGCATGAAGGTGATCGACCTCGGCGCGGCTCCC
GGCGGCTGGTGCCAAGTGGCGGCTGCCAGCGTGAAATCCGATCCCGCAAAGCCTTCCGTCGTCGGCATCGACTATCTCGA
AATGGACCCCGTGCCAGGAGCCGCGGTGCTCCAGATGGATTTTCTCGACGACGCCGCGCCGGACCGGCTGATCGAGGTTC
TGGGCGGGTCGCCGGATGTGGTCCTGTCCGATATGGCGGCACCCACCACAGGACACCGGCGGACCGATCACCTGCGCACC
ATGCAGCTTTGCGAGGCGGCGGCGGATTTTGCAATCTCCGTGTTGAAGACCGGCGGAAACTTCCTGGCCAAGACGTTCCA
GGGCGGTACGGAGGGCGAGCTCTTGTCGCTGCTCAAAAGGAATTTCCGTTCGGTCCATCACGTCAAGCCGCCTGCTTCCC
GCGATGAATCGGTCGAGCTTTACATTCTTGCAAAGGACTTCAAAGGCCGTCAGGCTGCGGAGGGCTGA

Upstream 100 bases:

>100_bases
TGCGGGTCGCCGACCGGGGCCTGAGGGAGGGCATGCTCAACGAGATGATGGCGCGCGACGGTGTTTGGCGGCGCCGAAAG
CAGAACGGAGCATGCCCGGC

Downstream 100 bases:

>100_bases
GCAGTCCGTTCCGCGGCCAAGGCCTGTTCCTCTTCTGCCACGCGCTTGCGGCCGTGGCCGGATACGGCACTTCCGGTGTC
GGCGGGAAGACGCAGGAACG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTKEKKAGSGTRVLKTRVKKKRGLKSSSRRWLERHLNDPYVRRANSEGMRSRAAYKLIEIDDKHHILAPGMKVIDLGAAP
GGWCQVAAASVKSDPAKPSVVGIDYLEMDPVPGAAVLQMDFLDDAAPDRLIEVLGGSPDVVLSDMAAPTTGHRRTDHLRT
MQLCEAAADFAISVLKTGGNFLAKTFQGGTEGELLSLLKRNFRSVHHVKPPASRDESVELYILAKDFKGRQAAEG

Sequences:

>Translated_235_residues
MTKEKKAGSGTRVLKTRVKKKRGLKSSSRRWLERHLNDPYVRRANSEGMRSRAAYKLIEIDDKHHILAPGMKVIDLGAAP
GGWCQVAAASVKSDPAKPSVVGIDYLEMDPVPGAAVLQMDFLDDAAPDRLIEVLGGSPDVVLSDMAAPTTGHRRTDHLRT
MQLCEAAADFAISVLKTGGNFLAKTFQGGTEGELLSLLKRNFRSVHHVKPPASRDESVELYILAKDFKGRQAAEG
>Mature_234_residues
TKEKKAGSGTRVLKTRVKKKRGLKSSSRRWLERHLNDPYVRRANSEGMRSRAAYKLIEIDDKHHILAPGMKVIDLGAAPG
GWCQVAAASVKSDPAKPSVVGIDYLEMDPVPGAAVLQMDFLDDAAPDRLIEVLGGSPDVVLSDMAAPTTGHRRTDHLRTM
QLCEAAADFAISVLKTGGNFLAKTFQGGTEGELLSLLKRNFRSVHHVKPPASRDESVELYILAKDFKGRQAAEG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=229, Percent_Identity=41.4847161572052, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=34.5549738219895, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=34.5549738219895, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=34.5549738219895, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI194097365, Length=209, Percent_Identity=34.4497607655502, Blast_Score=107, Evalue=7e-24,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=42.2330097087379, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553474, Length=206, Percent_Identity=36.4077669902913, Blast_Score=127, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=35.3846153846154, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=32.1243523316062, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=32.1243523316062, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=32.1243523316062, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=195, Percent_Identity=35.3846153846154, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=195, Percent_Identity=33.8461538461538, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=100, Percent_Identity=40, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356387, Length=227, Percent_Identity=37.4449339207048, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=34.8484848484849, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648639, Length=197, Percent_Identity=31.4720812182741, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859957, Length=192, Percent_Identity=34.375, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_MESSB (Q11L51)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673039.1
- ProteinModelPortal:   Q11L51
- SMR:   Q11L51
- STRING:   Q11L51
- GeneID:   4181105
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_0470
- NMPDR:   fig|266779.1.peg.2615
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q11L51
- ProtClustDB:   CLSK506964
- BioCyc:   MSP266779:MESO_0470-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 25613; Mature: 25482

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: ACT_SITE 184-184 BINDING 81-81 BINDING 83-83 BINDING 104-104 BINDING 120-120 BINDING 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEKKAGSGTRVLKTRVKKKRGLKSSSRRWLERHLNDPYVRRANSEGMRSRAAYKLIEI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHEEEEEE
DDKHHILAPGMKVIDLGAAPGGWCQVAAASVKSDPAKPSVVGIDYLEMDPVPGAAVLQMD
CCCCEEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEECCCEECCCCCCCEEEEEH
FLDDAAPDRLIEVLGGSPDVVLSDMAAPTTGHRRTDHLRTMQLCEAAADFAISVLKTGGN
HHCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
FLAKTFQGGTEGELLSLLKRNFRSVHHVKPPASRDESVELYILAKDFKGRQAAEG
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TKEKKAGSGTRVLKTRVKKKRGLKSSSRRWLERHLNDPYVRRANSEGMRSRAAYKLIEI
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHEEEEEE
DDKHHILAPGMKVIDLGAAPGGWCQVAAASVKSDPAKPSVVGIDYLEMDPVPGAAVLQMD
CCCCEEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEECCCEECCCCCCCEEEEEH
FLDDAAPDRLIEVLGGSPDVVLSDMAAPTTGHRRTDHLRTMQLCEAAADFAISVLKTGGN
HHCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
FLAKTFQGGTEGELLSLLKRNFRSVHHVKPPASRDESVELYILAKDFKGRQAAEG
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA