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The map label for this gene is yfiY [H]

Identifier: 110632495

GI number: 110632495

Start: 163200

End: 164120

Strand: Reverse

Name: yfiY [H]

Synonym: Meso_0133

Alternate gene names: 110632495

Gene position: 164120-163200 (Counterclockwise)

Preceding gene: 110632500

Following gene: 110632489

Centisome position: 3.72

GC content: 62.21

Gene sequence:

>921_bases
ATGAACCGTCGCCGGCTGCTCTCCTCCCTCCTGGCCCTGCCGCTCCTTTTTGCTGCTTCGGCTGCCCTTCAAGCCGCGGA
GATCACGCATTCGATGGGCACGACCGAGGTTCCGGATCATCCGCAGCGCGTGGTGGTGCTCACCAATGAGGGAACCGAGG
CTCTGCTGGCGGTGGACATCAAGCCCGTCGGCGCTGTCCGCTCCTGGCTGGGCGATCCGTGGTATGCGCATATCGCCGAT
CGGATGGAAGGCGTCGAAGTCGTCGGCGAGGAATCGGCCGTTAATCTCGAACTCATTGCCGCGCTCCAGCCCGATCTCAT
CATCGGCAACAAAACGCGCCAGGAGAAGATCTATGAGCAGCTTTCGGCCATCGCGCCCACGGTGTTCGCGGAGCGGCTGC
GCGGTGACTGGAAGATCAATTTCGCGCTCTATACCAAAGCCGTAGGCAAGGAGACGGAGGGTAAAGCCGTGCTCGACCGC
TACAATTCCCGCGTGGCGGCGTTGAGCGAGGCGCTCGGCCACCGCAAGCAGGAGAAGATCTCGCTCGTGCGCTTCATGCC
CGGCCGTACACGCATCTACTACAAGGACACCTTCCCCGGCCTTATCCTATCCGAAATCGGTTTCGCGCGGCCCGCCAACC
AGGACAAGGATACATTCGCCGACGAGGTGACGAAGGAGCGCATTCCGGAAATGGAGGGCGACCGCCTGTTCTACTTCGTC
TACGAGACCGGCGACGGCAGTGCCGAGACCCTGGCCGCAGAGTGGACCAAGGAGCCGCTTTGGCAGAACCTGGAAGTGGT
GAAGGCCGGAAAGGCGATGGCGGTGGATGATGCCATCTGGAACACGGCGGGTGGCGTGATCGCGGCCGATCTCATGATTG
GGCAACTCGCGGAGATCTACGGAATCTCACTGCCGGAGTGA

Upstream 100 bases:

>100_bases
ACAATGTGGGCGGATCTGCTCAAAGGTGATGTTGACAGTCAAGTTTGCTTCTGCTCTTGCTCTCCCAAATTCTGACCATG
CTTGTCAAGTTAGGTGCCCC

Downstream 100 bases:

>100_bases
TTTCATCTTCACTCCATTCTGCGTCGGAAGAGCCATGCCGCAGCCGCGAGCGTGAGAAAGGCGATGATGGCGAGCGGGAT
CGTGTTGTTCATCGCTTCGT

Product: periplasmic binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNRRRLLSSLLALPLLFAASAALQAAEITHSMGTTEVPDHPQRVVVLTNEGTEALLAVDIKPVGAVRSWLGDPWYAHIAD
RMEGVEVVGEESAVNLELIAALQPDLIIGNKTRQEKIYEQLSAIAPTVFAERLRGDWKINFALYTKAVGKETEGKAVLDR
YNSRVAALSEALGHRKQEKISLVRFMPGRTRIYYKDTFPGLILSEIGFARPANQDKDTFADEVTKERIPEMEGDRLFYFV
YETGDGSAETLAAEWTKEPLWQNLEVVKAGKAMAVDDAIWNTAGGVIAADLMIGQLAEIYGISLPE

Sequences:

>Translated_306_residues
MNRRRLLSSLLALPLLFAASAALQAAEITHSMGTTEVPDHPQRVVVLTNEGTEALLAVDIKPVGAVRSWLGDPWYAHIAD
RMEGVEVVGEESAVNLELIAALQPDLIIGNKTRQEKIYEQLSAIAPTVFAERLRGDWKINFALYTKAVGKETEGKAVLDR
YNSRVAALSEALGHRKQEKISLVRFMPGRTRIYYKDTFPGLILSEIGFARPANQDKDTFADEVTKERIPEMEGDRLFYFV
YETGDGSAETLAAEWTKEPLWQNLEVVKAGKAMAVDDAIWNTAGGVIAADLMIGQLAEIYGISLPE
>Mature_306_residues
MNRRRLLSSLLALPLLFAASAALQAAEITHSMGTTEVPDHPQRVVVLTNEGTEALLAVDIKPVGAVRSWLGDPWYAHIAD
RMEGVEVVGEESAVNLELIAALQPDLIIGNKTRQEKIYEQLSAIAPTVFAERLRGDWKINFALYTKAVGKETEGKAVLDR
YNSRVAALSEALGHRKQEKISLVRFMPGRTRIYYKDTFPGLILSEIGFARPANQDKDTFADEVTKERIPEMEGDRLFYFV
YETGDGSAETLAAEWTKEPLWQNLEVVKAGKAMAVDDAIWNTAGGVIAADLMIGQLAEIYGISLPE

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=289, Percent_Identity=25.9515570934256, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 33788; Mature: 33788

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRRLLSSLLALPLLFAASAALQAAEITHSMGTTEVPDHPQRVVVLTNEGTEALLAVDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEC
KPVGAVRSWLGDPWYAHIADRMEGVEVVGEESAVNLELIAALQPDLIIGNKTRQEKIYEQ
CCHHHHHHHHCCCHHHHHHHHHCCHHEECCCCCCCEEEEEECCCCEEECCCHHHHHHHHH
LSAIAPTVFAERLRGDWKINFALYTKAVGKETEGKAVLDRYNSRVAALSEALGHRKQEKI
HHHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
SLVRFMPGRTRIYYKDTFPGLILSEIGFARPANQDKDTFADEVTKERIPEMEGDRLFYFV
HHHEECCCCEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
YETGDGSAETLAAEWTKEPLWQNLEVVKAGKAMAVDDAIWNTAGGVIAADLMIGQLAEIY
EECCCCCHHHHHHHHHHHHHHCCCHHHHCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHH
GISLPE
CCCCCC
>Mature Secondary Structure
MNRRRLLSSLLALPLLFAASAALQAAEITHSMGTTEVPDHPQRVVVLTNEGTEALLAVDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEC
KPVGAVRSWLGDPWYAHIADRMEGVEVVGEESAVNLELIAALQPDLIIGNKTRQEKIYEQ
CCHHHHHHHHCCCHHHHHHHHHCCHHEECCCCCCCEEEEEECCCCEEECCCHHHHHHHHH
LSAIAPTVFAERLRGDWKINFALYTKAVGKETEGKAVLDRYNSRVAALSEALGHRKQEKI
HHHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
SLVRFMPGRTRIYYKDTFPGLILSEIGFARPANQDKDTFADEVTKERIPEMEGDRLFYFV
HHHEECCCCEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
YETGDGSAETLAAEWTKEPLWQNLEVVKAGKAMAVDDAIWNTAGGVIAADLMIGQLAEIY
EECCCCCHHHHHHHHHHHHHHCCCHHHHCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHH
GISLPE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]