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The map label for this gene is fadA [H]

Identifier: 110632384

GI number: 110632384

Start: 23226

End: 24434

Strand: Reverse

Name: fadA [H]

Synonym: Meso_0022

Alternate gene names: 110632384

Gene position: 24434-23226 (Counterclockwise)

Preceding gene: 110632385

Following gene: 110632383

Centisome position: 0.55

GC content: 62.7

Gene sequence:

>1209_bases
ATGCCTGATGCCTATGTCTACGATGCGGTGCGAAGCCCGCGCGGCCGCGGCAAAAAGGACGGCGCGCTGCACGAGGTGCC
GGCGGTTCGCCTGGCGGCGAAGATGCTGGAAGCGGTTCGCGACCGTAACGGGCTCGACACAAAGCTGGTGGACGACATCA
TCTTCGGCTGCGTCGATCCTGTGGGCGAGGCAGGTGCGGTGATACCCCGCTCGGCGGCCTTCGAGGCGGGTTACGACAAC
CGCGCGCCGGGTATGCAGATTTCCCGCTTCTGCGCTTCCGGACTTGACGCGATCAATTTAGGGGCGGCCAAAATTGCGGG
CGGCTCGGACGAGATCGTGATCGCAGGCGGCGTAGAGTCGATGTCGCGCGTCGGCATGGGCATGTCGGGCGGCGCGTGGT
TCATGGACCCGTCCGTCGGCCTGCCCGCATGGTTCATGCCGCAAGGCGTCTCCGCCGATCTTATCGCCACTAAATACGGC
TTTTCCCGCGACGACGTGGATGCCTATGCCGTGCAAAGCCAGAAGCGGGCGAAAAGGGCCTGGGACAAGGGCCATTTCAA
GAATTCGGTGGTCTCCATCAAGGATCAGAACGGTCTCACCATTCTCGATCAGGACGAGCACATGCGCCCAGACACGGATA
TGCAGTCGCTGGCCTCGCTCAACCCGTCCTTCGTGGTGCCCGGCGAAATGGGCGGCTTCGATGCGGTAGCAGTGCAGCGA
TATCCCGAACTCGAGGGCATCAACCACGTCCACCATGCCGGCAACTCATCGGGGATCGTGGACGGAGCGGCAGCAGTGCT
GCTTGGCTCGAAAAAGGCCGGCAAGTCGATGGGCCTGAAGCCGCGCGCGCGGATCCGTGCCTTCACCAATATCGGCTCCG
AGCCGGCCATCATGCTCACCGGCCCGGTGGACGTGACCAAGAAGCTTCTGAAAAAGGCAAAGATGAAGCTTTCCGACATC
GATCTCTTCGAGCTCAACGAGGCCTTCGCCTCGGTGGTGCTGCGCTACATCCAGGCCTTCGACATCGATGGCGACAAGAT
CAACGTAAATGGCGGCGCTATCGCGATGGGCCATCCGCTCGGCGCCACCGGTGCCATGATCTTCGGCACGGTACTCGACG
AATTGGAGAGGCGTGACATGAACACCGCGCTCGTCACGCTCTGCATCGGCGCCGGAATGGGCACGGCCACCATCATCGAG
CGGGTCTAG

Upstream 100 bases:

>100_bases
CGCATCTGGCGCGGATTTCCTCCGGCTCCGAGACGATGATGGCTATGACCGAAGCGGCGTTCTAGAGTAGCGCGAGCAGG
TAAGGATCGAGGAGACGGGA

Downstream 100 bases:

>100_bases
GCGGGAGAGGGCAATATCATGGCTGGCTACAAGAACTTCACCGTCGAGGTCGATAGCGAGGGTATCGCACTCGTCACCTG
GAACATGCCGGACCGCTCGA

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPDAYVYDAVRSPRGRGKKDGALHEVPAVRLAAKMLEAVRDRNGLDTKLVDDIIFGCVDPVGEAGAVIPRSAAFEAGYDN
RAPGMQISRFCASGLDAINLGAAKIAGGSDEIVIAGGVESMSRVGMGMSGGAWFMDPSVGLPAWFMPQGVSADLIATKYG
FSRDDVDAYAVQSQKRAKRAWDKGHFKNSVVSIKDQNGLTILDQDEHMRPDTDMQSLASLNPSFVVPGEMGGFDAVAVQR
YPELEGINHVHHAGNSSGIVDGAAAVLLGSKKAGKSMGLKPRARIRAFTNIGSEPAIMLTGPVDVTKKLLKKAKMKLSDI
DLFELNEAFASVVLRYIQAFDIDGDKINVNGGAIAMGHPLGATGAMIFGTVLDELERRDMNTALVTLCIGAGMGTATIIE
RV

Sequences:

>Translated_402_residues
MPDAYVYDAVRSPRGRGKKDGALHEVPAVRLAAKMLEAVRDRNGLDTKLVDDIIFGCVDPVGEAGAVIPRSAAFEAGYDN
RAPGMQISRFCASGLDAINLGAAKIAGGSDEIVIAGGVESMSRVGMGMSGGAWFMDPSVGLPAWFMPQGVSADLIATKYG
FSRDDVDAYAVQSQKRAKRAWDKGHFKNSVVSIKDQNGLTILDQDEHMRPDTDMQSLASLNPSFVVPGEMGGFDAVAVQR
YPELEGINHVHHAGNSSGIVDGAAAVLLGSKKAGKSMGLKPRARIRAFTNIGSEPAIMLTGPVDVTKKLLKKAKMKLSDI
DLFELNEAFASVVLRYIQAFDIDGDKINVNGGAIAMGHPLGATGAMIFGTVLDELERRDMNTALVTLCIGAGMGTATIIE
RV
>Mature_401_residues
PDAYVYDAVRSPRGRGKKDGALHEVPAVRLAAKMLEAVRDRNGLDTKLVDDIIFGCVDPVGEAGAVIPRSAAFEAGYDNR
APGMQISRFCASGLDAINLGAAKIAGGSDEIVIAGGVESMSRVGMGMSGGAWFMDPSVGLPAWFMPQGVSADLIATKYGF
SRDDVDAYAVQSQKRAKRAWDKGHFKNSVVSIKDQNGLTILDQDEHMRPDTDMQSLASLNPSFVVPGEMGGFDAVAVQRY
PELEGINHVHHAGNSSGIVDGAAAVLLGSKKAGKSMGLKPRARIRAFTNIGSEPAIMLTGPVDVTKKLLKKAKMKLSDID
LFELNEAFASVVLRYIQAFDIDGDKINVNGGAIAMGHPLGATGAMIFGTVLDELERRDMNTALVTLCIGAGMGTATIIER
V

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=35.1758793969849, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI167614485, Length=415, Percent_Identity=34.2168674698795, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI4501853, Length=408, Percent_Identity=34.5588235294118, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4557237, Length=420, Percent_Identity=30.7142857142857, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=29.6629213483146, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=46.0674157303371, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI48994986, Length=413, Percent_Identity=38.498789346247, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=37.3239436619718, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082165, Length=415, Percent_Identity=37.3493975903614, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1788554, Length=417, Percent_Identity=33.3333333333333, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788683, Length=428, Percent_Identity=28.0373831775701, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI133906874, Length=396, Percent_Identity=34.0909090909091, Blast_Score=207, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI25147385, Length=413, Percent_Identity=29.7820823244552, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=413, Percent_Identity=29.2978208232446, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17551802, Length=450, Percent_Identity=28.6666666666667, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17535917, Length=412, Percent_Identity=26.6990291262136, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=406, Percent_Identity=33.4975369458128, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6325229, Length=417, Percent_Identity=31.1750599520384, Blast_Score=156, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24655093, Length=416, Percent_Identity=36.7788461538462, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17648125, Length=413, Percent_Identity=34.8668280871671, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24640423, Length=416, Percent_Identity=31.0096153846154, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI17137578, Length=447, Percent_Identity=31.3199105145414, Blast_Score=168, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42596; Mature: 42465

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAYVYDAVRSPRGRGKKDGALHEVPAVRLAAKMLEAVRDRNGLDTKLVDDIIFGCVDP
CCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
VGEAGAVIPRSAAFEAGYDNRAPGMQISRFCASGLDAINLGAAKIAGGSDEIVIAGGVES
CCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCEECCCCEEECCCCCCEEEECCHHH
MSRVGMGMSGGAWFMDPSVGLPAWFMPQGVSADLIATKYGFSRDDVDAYAVQSQKRAKRA
HHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCHHHECCCCCCCCCHHHHHHHHHHHHHH
WDKGHFKNSVVSIKDQNGLTILDQDEHMRPDTDMQSLASLNPSFVVPGEMGGFDAVAVQR
HHHCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHH
YPELEGINHVHHAGNSSGIVDGAAAVLLGSKKAGKSMGLKPRARIRAFTNIGSEPAIMLT
CCCCCCCCHHHHCCCCCCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEE
GPVDVTKKLLKKAKMKLSDIDLFELNEAFASVVLRYIQAFDIDGDKINVNGGAIAMGHPL
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCC
GATGAMIFGTVLDELERRDMNTALVTLCIGAGMGTATIIERV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
PDAYVYDAVRSPRGRGKKDGALHEVPAVRLAAKMLEAVRDRNGLDTKLVDDIIFGCVDP
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
VGEAGAVIPRSAAFEAGYDNRAPGMQISRFCASGLDAINLGAAKIAGGSDEIVIAGGVES
CCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCEECCCCEEECCCCCCEEEECCHHH
MSRVGMGMSGGAWFMDPSVGLPAWFMPQGVSADLIATKYGFSRDDVDAYAVQSQKRAKRA
HHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCHHHECCCCCCCCCHHHHHHHHHHHHHH
WDKGHFKNSVVSIKDQNGLTILDQDEHMRPDTDMQSLASLNPSFVVPGEMGGFDAVAVQR
HHHCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHH
YPELEGINHVHHAGNSSGIVDGAAAVLLGSKKAGKSMGLKPRARIRAFTNIGSEPAIMLT
CCCCCCCCHHHHCCCCCCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEE
GPVDVTKKLLKKAKMKLSDIDLFELNEAFASVVLRYIQAFDIDGDKINVNGGAIAMGHPL
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCC
GATGAMIFGTVLDELERRDMNTALVTLCIGAGMGTATIIERV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]