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The map label for this gene is ilvE [H]

Identifier: 109948185

GI number: 109948185

Start: 1489016

End: 1490038

Strand: Reverse

Name: ilvE [H]

Synonym: Hac_1717

Alternate gene names: 109948185

Gene position: 1490038-1489016 (Counterclockwise)

Preceding gene: 109948186

Following gene: 109948183

Centisome position: 95.89

GC content: 43.4

Gene sequence:

>1023_bases
ATGGCAAATTTAGAAAATTTGGACTGGAAAAATCTGGGCTTTAGCTACATTAAAACGGATTTTCGCTTCATCGCTACTTA
TAAAAACGGCTCTTGGTCACAGGGCGAGTTAGTCAGCGAAAATGTGTTACAGATTAGTGAAGGCTCTCCGGTCTTGCATT
ATGGGCAGGCTTGTTTTGAAGGCTTGAAAGCGTATCGCTCTCAAGATGGAAAAGCCCTACTCTTTCGCCCTTTAGAAAAC
GCCAAACGCTTGCAAACTTCATGCGAAAGATTGCTCATGCCAAAAGTGAGCGAAGAGTTGTTTTTAAGGGCATGCACTGA
AGCGATCAAAGCAAACATGAAATGGCTCGCTCCTTACAAAAGCGGGGCGAGTTTGTATTTGCGCCCTTTTGTCATAGGCG
TAGGGGATAATTTGGGGGTAAAGCCGGCCAATGAATACCTTTTTATCGTATTTTGTGTGCCTGTGGGGGCGTATTTTAAG
GGGGGTATAGAAAAAGGGGGGGCGAGATTTATCACCACGACTTTTGATAGAGCCGCGCCTAAAGGCACAGGGGGGGTGAA
AGTGGGGGGGAATTATGCTGCAAGCTTATTGGCCCATAAAATGGCCCTAGAGCAAGGCTATGATGATTGCATTTATTTAG
ACCCTACCACGCACACTAAAATTGAAGAAGTGGGGGCGGCGAATTTTTTTGGCATCACGCATGATAATACTTTTATCACT
CCGCATTCGCCGAGCGTTCTACCAAGCATTACCAGAAAAAGCTTGATGTTTTTGGCTCAAGAATATTTGGGTTTGAAAGT
GGAAGAAAAAGAAATTTTGATTGATAAATTAGATGTGTTTAAAGAAGCTGGAGCGTGCGGGACAGCTGCGATCATCACGC
CTATTAAAGAAATTGTGCACCACAACAAGTCTTATTTTTTTGAAACGCCAGGTCATGTTACTAAGCGACTCTATGATTTG
CTTTTATCCATCCAACAAGGCGAACAAAAAGCCCCTAAAGATTGGGTGCTTGAAGTTAATTAA

Upstream 100 bases:

>100_bases
CCCCATTTTAAATTAAATGGAGTTACTTTGAGCGTTAATATTTGAGGGATTTTTAACCCCTTTTAGATACAATCGCATCT
AAAACTGTTAAAGGTGCAAA

Downstream 100 bases:

>100_bases
ACATTTTGTTTAAGAACGCTCCAAATAAGTCTCTTTGAGGCTAGCCTTAGCCCTTTGCCAATTGGGTAAATACAAACTCA
ACAAATCTCTAAGATATTCG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MANLENLDWKNLGFSYIKTDFRFIATYKNGSWSQGELVSENVLQISEGSPVLHYGQACFEGLKAYRSQDGKALLFRPLEN
AKRLQTSCERLLMPKVSEELFLRACTEAIKANMKWLAPYKSGASLYLRPFVIGVGDNLGVKPANEYLFIVFCVPVGAYFK
GGIEKGGARFITTTFDRAAPKGTGGVKVGGNYAASLLAHKMALEQGYDDCIYLDPTTHTKIEEVGAANFFGITHDNTFIT
PHSPSVLPSITRKSLMFLAQEYLGLKVEEKEILIDKLDVFKEAGACGTAAIITPIKEIVHHNKSYFFETPGHVTKRLYDL
LLSIQQGEQKAPKDWVLEVN

Sequences:

>Translated_340_residues
MANLENLDWKNLGFSYIKTDFRFIATYKNGSWSQGELVSENVLQISEGSPVLHYGQACFEGLKAYRSQDGKALLFRPLEN
AKRLQTSCERLLMPKVSEELFLRACTEAIKANMKWLAPYKSGASLYLRPFVIGVGDNLGVKPANEYLFIVFCVPVGAYFK
GGIEKGGARFITTTFDRAAPKGTGGVKVGGNYAASLLAHKMALEQGYDDCIYLDPTTHTKIEEVGAANFFGITHDNTFIT
PHSPSVLPSITRKSLMFLAQEYLGLKVEEKEILIDKLDVFKEAGACGTAAIITPIKEIVHHNKSYFFETPGHVTKRLYDL
LLSIQQGEQKAPKDWVLEVN
>Mature_339_residues
ANLENLDWKNLGFSYIKTDFRFIATYKNGSWSQGELVSENVLQISEGSPVLHYGQACFEGLKAYRSQDGKALLFRPLENA
KRLQTSCERLLMPKVSEELFLRACTEAIKANMKWLAPYKSGASLYLRPFVIGVGDNLGVKPANEYLFIVFCVPVGAYFKG
GIEKGGARFITTTFDRAAPKGTGGVKVGGNYAASLLAHKMALEQGYDDCIYLDPTTHTKIEEVGAANFFGITHDNTFITP
HSPSVLPSITRKSLMFLAQEYLGLKVEEKEILIDKLDVFKEAGACGTAAIITPIKEIVHHNKSYFFETPGHVTKRLYDLL
LSIQQGEQKAPKDWVLEVN

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=348, Percent_Identity=31.0344827586207, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI296010906, Length=348, Percent_Identity=31.0344827586207, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI38176287, Length=348, Percent_Identity=31.0344827586207, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI50658084, Length=310, Percent_Identity=30.9677419354839, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI258614015, Length=263, Percent_Identity=32.319391634981, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI296010902, Length=348, Percent_Identity=26.4367816091954, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI296010900, Length=254, Percent_Identity=30.3149606299213, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI48994963, Length=319, Percent_Identity=31.974921630094, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=314, Percent_Identity=31.8471337579618, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17565728, Length=271, Percent_Identity=33.9483394833948, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322608, Length=318, Percent_Identity=32.7044025157233, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322002, Length=318, Percent_Identity=31.4465408805031, Blast_Score=135, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24641779, Length=350, Percent_Identity=29.7142857142857, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37778; Mature: 37647

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLENLDWKNLGFSYIKTDFRFIATYKNGSWSQGELVSENVLQISEGSPVLHYGQACFE
CCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCCHHHCCCEEECCCCCHHHHHHHHHH
GLKAYRSQDGKALLFRPLENAKRLQTSCERLLMPKVSEELFLRACTEAIKANMKWLAPYK
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECC
SGASLYLRPFVIGVGDNLGVKPANEYLFIVFCVPVGAYFKGGIEKGGARFITTTFDRAAP
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEECHHHHHHCCCCCCCCEEEEEEHHHCCC
KGTGGVKVGGNYAASLLAHKMALEQGYDDCIYLDPTTHTKIEEVGAANFFGITHDNTFIT
CCCCCEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCEEEEEECCCEEEC
PHSPSVLPSITRKSLMFLAQEYLGLKVEEKEILIDKLDVFKEAGACGTAAIITPIKEIVH
CCCCCCCHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HNKSYFFETPGHVTKRLYDLLLSIQQGEQKAPKDWVLEVN
CCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
>Mature Secondary Structure 
ANLENLDWKNLGFSYIKTDFRFIATYKNGSWSQGELVSENVLQISEGSPVLHYGQACFE
CCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCCHHHCCCEEECCCCCHHHHHHHHHH
GLKAYRSQDGKALLFRPLENAKRLQTSCERLLMPKVSEELFLRACTEAIKANMKWLAPYK
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECC
SGASLYLRPFVIGVGDNLGVKPANEYLFIVFCVPVGAYFKGGIEKGGARFITTTFDRAAP
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEECHHHHHHCCCCCCCCEEEEEEHHHCCC
KGTGGVKVGGNYAASLLAHKMALEQGYDDCIYLDPTTHTKIEEVGAANFFGITHDNTFIT
CCCCCEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCEEEEEECCCEEEC
PHSPSVLPSITRKSLMFLAQEYLGLKVEEKEILIDKLDVFKEAGACGTAAIITPIKEIVH
CCCCCCCHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HNKSYFFETPGHVTKRLYDLLLSIQQGEQKAPKDWVLEVN
CCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]