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The map label for this gene is purU [H]

Identifier: 109948134

GI number: 109948134

Start: 1444675

End: 1445556

Strand: Reverse

Name: purU [H]

Synonym: Hac_1657

Alternate gene names: 109948134

Gene position: 1445556-1444675 (Counterclockwise)

Preceding gene: 109948135

Following gene: 109948133

Centisome position: 93.03

GC content: 37.64

Gene sequence:

>882_bases
ATGTTAGAATTCATTTTAAAATTCCAAGCCAAAGACGCTAAAGGCCTGGTGAGTGCGATTAGCACCACCATCGCTAACAA
GGGCTATAATATTGTCAAAAACGATGAATTTGTTGATCCTTTAAAACAACGCTTTTTCATGCGCTTAAAAATCCAAAAAG
AAATTAAGCCCTTGAACACTGAAATTAAAGAACAAGAAGAGTGGGTCTTAAAAACCGCTCTTTTTAAAGCTTTAGAAAAT
TTTAAAGCGCTGTTGATTGAAGTCATTTTAATGCGCAAAAAAAACATTGTTTTACTCGCCACTAAAGAGAGCCATTGTTT
AGGGGATCTGCTTTTAAGGGTGTATGGGGGGGAATTAAACGCTCAAATTTTAGGCGTTATCGCCAATTACGAGATTTTAC
GCCCTTTAGTGGAAAAATTTGACATCCCTTATTTTTATGCACCTTGCGCTAACCAAATTTTGCATGAAAAAGAAGTTTTA
GCGATCATCAAAAATTTGGAATCAGAGCATCAAACAAGCATAGACTTGCTCGTTTTAGCCAAATACATGCGCATTTTAAG
CCATGATTTTACAAAGCGTTATGAAAACCAGATCCTAAATATCCATCATAGTTTTTTACCCGCATTCATTGGGGCTAACC
CTTACCAGCAAGCCTTTGAAAGAGGCGTGAAAGTCATTGGGGCTACGGCACATTTTGTGAATGAAAGCCTTGATGCAGGG
CCTATTATTTTACAAGACACTTTACCCATCAACCACAATTACAGCGTGGAAAAAATGCGTTTGGTGGGTAAGGATATAGA
AAAACTGGTTTTGGCTAGGGCGTTAAAACTGGTTTTAGAAGACAGAGTCTTTGTGCATGAAAACAAAACGGTGGTGTTTT
GA

Upstream 100 bases:

>100_bases
CTAGAAAAAAGCCCTATGGAGCGTTTTATTGAAAAAGCCACGCAATCAGCGACAAATATCATCACGCAAGCGCTTGGGTA
TCAATTATTGATGAGGTAAG

Downstream 100 bases:

>100_bases
ATGCTTTTAGATCTCATCAACCTCAATGAAGAAACCCTAAAAGAAAGGATCAAAACCGAATTCTTTAAGGATAAGAAATT
CCTTTATAGTGGGGATAAGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLEFILKFQAKDAKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNTEIKEQEEWVLKTALFKALEN
FKALLIEVILMRKKNIVLLATKESHCLGDLLLRVYGGELNAQILGVIANYEILRPLVEKFDIPYFYAPCANQILHEKEVL
AIIKNLESEHQTSIDLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
PIILQDTLPINHNYSVEKMRLVGKDIEKLVLARALKLVLEDRVFVHENKTVVF

Sequences:

>Translated_293_residues
MLEFILKFQAKDAKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNTEIKEQEEWVLKTALFKALEN
FKALLIEVILMRKKNIVLLATKESHCLGDLLLRVYGGELNAQILGVIANYEILRPLVEKFDIPYFYAPCANQILHEKEVL
AIIKNLESEHQTSIDLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
PIILQDTLPINHNYSVEKMRLVGKDIEKLVLARALKLVLEDRVFVHENKTVVF
>Mature_293_residues
MLEFILKFQAKDAKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNTEIKEQEEWVLKTALFKALEN
FKALLIEVILMRKKNIVLLATKESHCLGDLLLRVYGGELNAQILGVIANYEILRPLVEKFDIPYFYAPCANQILHEKEVL
AIIKNLESEHQTSIDLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
PIILQDTLPINHNYSVEKMRLVGKDIEKLVLARALKLVLEDRVFVHENKTVVF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=121, Percent_Identity=42.1487603305785, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI209869995, Length=121, Percent_Identity=42.1487603305785, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI209869993, Length=121, Percent_Identity=42.1487603305785, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1787483, Length=289, Percent_Identity=43.598615916955, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1788846, Length=114, Percent_Identity=35.9649122807018, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=172, Percent_Identity=30.8139534883721, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24582400, Length=262, Percent_Identity=27.8625954198473, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33640; Mature: 33640

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFILKFQAKDAKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNT
CCHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
EIKEQEEWVLKTALFKALENFKALLIEVILMRKKNIVLLATKESHCLGDLLLRVYGGELN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
AQILGVIANYEILRPLVEKFDIPYFYAPCANQILHEKEVLAIIKNLESEHQTSIDLLVLA
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIILQDTLPINHNYSVEKMRLVGKDIEKLVLARALKLVLEDRVFVHENKTVVF
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEC
>Mature Secondary Structure
MLEFILKFQAKDAKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNT
CCHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
EIKEQEEWVLKTALFKALENFKALLIEVILMRKKNIVLLATKESHCLGDLLLRVYGGELN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
AQILGVIANYEILRPLVEKFDIPYFYAPCANQILHEKEVLAIIKNLESEHQTSIDLLVLA
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIILQDTLPINHNYSVEKMRLVGKDIEKLVLARALKLVLEDRVFVHENKTVVF
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8335255 [H]