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The map label for this gene is mboIBM [H]

Identifier: 109948003

GI number: 109948003

Start: 1314237

End: 1315073

Strand: Reverse

Name: mboIBM [H]

Synonym: Hac_1512

Alternate gene names: 109948003

Gene position: 1315073-1314237 (Counterclockwise)

Preceding gene: 109948004

Following gene: 109948000

Centisome position: 84.63

GC content: 37.63

Gene sequence:

>837_bases
TTGCCTTATTCTAATCAAACCCCACACTCCGTTTTTGAAAGCACGGACAAGCTTTTCACTCTCTATCAAGGGGATTGCAA
TGAGGTTTTGTCTCTATTTGAAAATACTTTTGATTTGATTTTTGCCGATCCGCCTTATTTCCTTTCTAATGATGGCTTAA
GCATACAGAGCGGTAAAATTGTGAGCGTCAATAAAGGCGATTGGGATAAAGAAGAGAGGATTAATGATATTGATGAGTTT
AATTATCAGTGGATAAACAGCGCTAAAAAAGCTCTAAAAAATACAGGAAGTCTTTTAATCAGCGGGACTTATCACAATCT
TTTTTCTTTGGGGCGTATTTTACAAAAACTGGATTTTAAGATTTTAAACCTCATCACTTGGCAAAAAACCAACCCTCCCC
CTAATTTTAGTTGCCGTTATTTGACGCATTCAGCCGAGCAAATCATTTGGGCGAGAAAAAGCCACAAACACAAGCATGTT
TTTAACTATGAGGTTTTAAAAAAGATCAATAGCGATAAACAAATGCGTGATGTATGGAGCTTCCCGGCTATCGCACCTTG
GGAAAAAACAAACGGCAAGCACCCCACTCAAAAACCGCTTGCGTTACTTGTGCGCTTGCTTTTAATGGCGAGTAATGAAA
ATTCTCTCATTGGCGATCCTTTTAGCGGGAGTTCCACCACAGGTATTGCGGCCAATCTTTTGAAGAGAAAATTTATCGGT
ATAGAAAAAGAAAGCGAATTTATTAAAATGTCAATAAATAGAAAAATGGAATTAGACGCTCGCTATAAAGAAATCCGATC
CAAAATCAAAGATTTGAATCCAATAGACCCCTTTTGA

Upstream 100 bases:

>100_bases
TGGCGATGTTTGGTTTTGATACAGTGGAAGGTGATGAAAAGATGCAAAAATACATTGAAGACAAAGGCATTAAAGCAGAG
TAGGGGGAGTAAAAAGCCAT

Downstream 100 bases:

>100_bases
GTGGGCTTAAGCGTTATACTTTTCGGATTTTATCTCAAAATGAGATTCTATTTTCACCCAATCCTCACAAAGGATATTTT
CATGCCCAAAAAGCCAGTGT

Product: DNA methyltransferase

Products: NA

Alternate protein names: M.MboIB; Adenine-specific methyltransferase MboIB; M.MboI C [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MPYSNQTPHSVFESTDKLFTLYQGDCNEVLSLFENTFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKEERINDIDEF
NYQWINSAKKALKNTGSLLISGTYHNLFSLGRILQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHKHV
FNYEVLKKINSDKQMRDVWSFPAIAPWEKTNGKHPTQKPLALLVRLLLMASNENSLIGDPFSGSSTTGIAANLLKRKFIG
IEKESEFIKMSINRKMELDARYKEIRSKIKDLNPIDPF

Sequences:

>Translated_278_residues
MPYSNQTPHSVFESTDKLFTLYQGDCNEVLSLFENTFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKEERINDIDEF
NYQWINSAKKALKNTGSLLISGTYHNLFSLGRILQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHKHV
FNYEVLKKINSDKQMRDVWSFPAIAPWEKTNGKHPTQKPLALLVRLLLMASNENSLIGDPFSGSSTTGIAANLLKRKFIG
IEKESEFIKMSINRKMELDARYKEIRSKIKDLNPIDPF
>Mature_277_residues
PYSNQTPHSVFESTDKLFTLYQGDCNEVLSLFENTFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKEERINDIDEFN
YQWINSAKKALKNTGSLLISGTYHNLFSLGRILQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHKHVF
NYEVLKKINSDKQMRDVWSFPAIAPWEKTNGKHPTQKPLALLVRLLLMASNENSLIGDPFSGSSTTGIAANLLKRKFIGI
EKESEFIKMSINRKMELDARYKEIRSKIKDLNPIDPF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=264, Percent_Identity=29.9242424242424, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32034; Mature: 31903

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYSNQTPHSVFESTDKLFTLYQGDCNEVLSLFENTFDLIFADPPYFLSNDGLSIQSGKI
CCCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHCCCEEEECCCCCEECCCCCEECCCEE
VSVNKGDWDKEERINDIDEFNYQWINSAKKALKNTGSLLISGTYHNLFSLGRILQKLDFK
EEECCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHH
ILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHKHVFNYEVLKKINSDKQMRDVWS
HHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHC
FPAIAPWEKTNGKHPTQKPLALLVRLLLMASNENSLIGDPFSGSSTTGIAANLLKRKFIG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHCC
IEKESEFIKMSINRKMELDARYKEIRSKIKDLNPIDPF
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PYSNQTPHSVFESTDKLFTLYQGDCNEVLSLFENTFDLIFADPPYFLSNDGLSIQSGKI
CCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHCCCEEEECCCCCEECCCCCEECCCEE
VSVNKGDWDKEERINDIDEFNYQWINSAKKALKNTGSLLISGTYHNLFSLGRILQKLDFK
EEECCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHH
ILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHKHVFNYEVLKKINSDKQMRDVWS
HHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHC
FPAIAPWEKTNGKHPTQKPLALLVRLLLMASNENSLIGDPFSGSSTTGIAANLLKRKFIG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHCC
IEKESEFIKMSINRKMELDARYKEIRSKIKDLNPIDPF
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]