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The map label for this gene is trmD [H]

Identifier: 109947825

GI number: 109947825

Start: 1133568

End: 1134242

Strand: Reverse

Name: trmD [H]

Synonym: Hac_1315

Alternate gene names: 109947825

Gene position: 1134242-1133568 (Counterclockwise)

Preceding gene: 109947826

Following gene: 109947824

Centisome position: 72.99

GC content: 38.52

Gene sequence:

>675_bases
TTGACTCTCTTTCCGCAACTCGTATGGTCTTATTTTCAAGACTCTATTTTAAAAAGAGCGTTAGAAAAAAATCTTTTTGC
ATTAGAAGTGTTAAACCTTAGGGATTTTAGTGCCAATAAATATCAAAAAGCGGATCACACACTCATTGGTGGGGGGGCAG
GGCAACTTTTAGACCCTGAGATGATAGAAAACGCGCTCAATTCTATTAAAAACCCTAAACATACGATTTTTTTAAGTGCG
GTGGGCAAGCCTTTCAAACAAATAGATGCGATGCGTTTGGCTCAAAAAAAGCATGTCGTTTTGGTGTGCGGGCGTTATGA
GGGCTTTGATGAACGCTCTATTGAACTTAGTGCTGATGAGGTTTTTTGTATAGGCGATTTTATTTTGACAGGGGGCGAGC
TTGGGGCGTTATGTTTGATGGATAGCATCGCTCGCCACATTCAAGGGGTTTTAGGTAACGCCCAATCTTTAGAGAGCGAG
AGCTTTGAAAATGATTATTTGGAGGCCCCTAATTTCGCTAACGCTGTTTTTAAATCCAAAGAAATCAATAAAATCCCTGC
ACCTTTAGAATATTCTAAAGGAAATCATGCTAAAATCAAGCAACTAAAACTTGATTTGTCAAAATTAAGGACAAAATTTT
ACCGCCTTGATTTATTCAAGCAACACAAATCATGA

Upstream 100 bases:

>100_bases
TAGGGATTTTTATATCAAAGAAATCCTTTTGCAAGACAAAAAAATTACCACCAACAACGCTAAAACGCTTTTAGAGAATA
GTTGAGTGAAATTCAGCGTT

Downstream 100 bases:

>100_bases
AATTGACATTAAGGAAAACACATGAAAAACCGCTACATTCAGCAATTTGAAGACGCTCAATTAAAAGATAAAGTCATGCC
GCAATTTAAAGCTGGTGATA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTLFPQLVWSYFQDSILKRALEKNLFALEVLNLRDFSANKYQKADHTLIGGGAGQLLDPEMIENALNSIKNPKHTIFLSA
VGKPFKQIDAMRLAQKKHVVLVCGRYEGFDERSIELSADEVFCIGDFILTGGELGALCLMDSIARHIQGVLGNAQSLESE
SFENDYLEAPNFANAVFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS

Sequences:

>Translated_224_residues
MTLFPQLVWSYFQDSILKRALEKNLFALEVLNLRDFSANKYQKADHTLIGGGAGQLLDPEMIENALNSIKNPKHTIFLSA
VGKPFKQIDAMRLAQKKHVVLVCGRYEGFDERSIELSADEVFCIGDFILTGGELGALCLMDSIARHIQGVLGNAQSLESE
SFENDYLEAPNFANAVFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS
>Mature_223_residues
TLFPQLVWSYFQDSILKRALEKNLFALEVLNLRDFSANKYQKADHTLIGGGAGQLLDPEMIENALNSIKNPKHTIFLSAV
GKPFKQIDAMRLAQKKHVVLVCGRYEGFDERSIELSADEVFCIGDFILTGGELGALCLMDSIARHIQGVLGNAQSLESES
FENDYLEAPNFANAVFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=32.5892857142857, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25251; Mature: 25120

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFPQLVWSYFQDSILKRALEKNLFALEVLNLRDFSANKYQKADHTLIGGGAGQLLDPE
CCCCHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHH
MIENALNSIKNPKHTIFLSAVGKPFKQIDAMRLAQKKHVVLVCGRYEGFDERSIELSADE
HHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCC
VFCIGDFILTGGELGALCLMDSIARHIQGVLGNAQSLESESFENDYLEAPNFANAVFKSK
EEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHCCC
EINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLFPQLVWSYFQDSILKRALEKNLFALEVLNLRDFSANKYQKADHTLIGGGAGQLLDPE
CCCHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHH
MIENALNSIKNPKHTIFLSAVGKPFKQIDAMRLAQKKHVVLVCGRYEGFDERSIELSADE
HHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCC
VFCIGDFILTGGELGALCLMDSIARHIQGVLGNAQSLESESFENDYLEAPNFANAVFKSK
EEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHCCC
EINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]