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The map label for this gene is glcD [H]

Identifier: 109947447

GI number: 109947447

Start: 781985

End: 783364

Strand: Reverse

Name: glcD [H]

Synonym: Hac_0898

Alternate gene names: 109947447

Gene position: 783364-781985 (Counterclockwise)

Preceding gene: 109947448

Following gene: 109947446

Centisome position: 50.41

GC content: 41.01

Gene sequence:

>1380_bases
ATGTTAGAGAAGCGACACCTTAAATATTTTAAAAATTTAGTAGGGGAAGAGGATTTCTTCACTGATTTAGCGCATTTGAA
CGCTTATTGTTATGACGCTACCAAAGAAAGGCATTTGCCTAGCGGCGTGATTTTCCCTAAAAACGAGCAAGAAATCAGCC
AAATTTTAAAATATTGCAACGAGCATCGCCTTATCGTTGTGCCTAGGGGGGCTGGGAGCGGTTTTACAGGGGGAGCGTTG
AGCGTGAGTGGGGGGCTAGTTTTAAGCGTAGAAAAGTATTTGGACAAAATTTTAGAGATTGACACTAAAAATCTAATCGC
TAGAGTAGAACCGGGTGTGATTAACAAGCATTTCCAAAACGAAGTGGAAAAATTAAACTTATTCTACCCCCCAGATCCAG
CGAGTGAGAATCAAAGCACTTTAGGGGGGAATGTCGCTGAAAATGCCGGTGGCATGCGTGCGGCCAAATACGGCATCACT
AAAGATTATGTCATGGCTTTAAGGGTGGTTTTAGCCAATGGCGAAGTGATAAGGGCAGGTAAAAAAACGATTAAGGATGT
CGCAGGCTTTAATGTTGCAGGGCTTATGATCGCTAGTGAGGGGTGTTTGGGCGTGATTTCTGAGATCACTCTAAAGCTTT
TAGCCAAACCGCCCCTAAAACAAAGCGCGATGGGGGTTTTTAATCATATTGAAGACGCCATGAACGCTGTTTATAAAACA
ATGAGTAGCGGTGTTACGCCTGTGGCGATGGAATTTTTGGATAATTTGAGTATTAAAGCGGTTGAAGAGAGGTTTTCTAA
AGGCTTGCCCAAAGACGCTGGAGCGATACTCATCACTCAAGTAGATGGCGTAGTTAAAGAGCAGATTGCATGGCAACTCA
ATGAGATAGAAAAGCATTTTAAAGCCAATGGTTGCGTTGGTTTTAAAGTGGCTCAAAACGAACAAGAAGAGCAGGATTTA
TGGTTTTCAAGGCGTAACGCTTCCCAAAGTATTAGCGTTTATGGTAAAAAAAAGTTGAATGAAGATGTTACCGTTCCTAG
GGCGAGTTTGCCTAGTTTGTTACAAGAAGTCGCTAAAATCAGCCAAAAATACGGTTTTAAAATCCCTTGCTTTGGCCATA
CAGGCGATGGCAATGTGCATGTGAATATCATGTTAGAAGATCCTAAAAAAGATTTAGAAAAAGGCCATAAGGCTATGGAA
GAGATTTTTCAAGCCGCTATTAATTTAGAGGGGACTTTAAGCGGGGAGCATGGCATAGGCTTGTCTAAGGCAAAATTCAT
GCCTTTAGCGTTTAATGATAGTGAAATGGAGCTTTTTAGGAATATTAAAAAAGCCCTTGATCCTAATAATATTTTAAACC
CTTTTAAAATGGGGTTGTAA

Upstream 100 bases:

>100_bases
GGCGAGAGAATTTGAACAAAGAGCGAGAGAACATCAAGAAAAAGATGAAGCAGAACTTGAGGAAAGAAGAAAGGCTTTAG
AGTCCAATAAGAAGTAGACC

Downstream 100 bases:

>100_bases
AAATAAAACAAGGAAGTCGCATGAAAATCGGTGTTTATGGAGCGAGCGGTCGTATAGGGAAATTACTCTTAGAAGAATTG
AAAGGGGGGTATAAAGGATT

Product: glycolate oxidase subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLEKRHLKYFKNLVGEEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKYLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGIT
KDYVMALRVVLANGEVIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVGFKVAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAME
EIFQAAINLEGTLSGEHGIGLSKAKFMPLAFNDSEMELFRNIKKALDPNNILNPFKMGL

Sequences:

>Translated_459_residues
MLEKRHLKYFKNLVGEEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKYLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGIT
KDYVMALRVVLANGEVIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVGFKVAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAME
EIFQAAINLEGTLSGEHGIGLSKAKFMPLAFNDSEMELFRNIKKALDPNNILNPFKMGL
>Mature_459_residues
MLEKRHLKYFKNLVGEEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKYLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGIT
KDYVMALRVVLANGEVIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVGFKVAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAME
EIFQAAINLEGTLSGEHGIGLSKAKFMPLAFNDSEMELFRNIKKALDPNNILNPFKMGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=425, Percent_Identity=31.2941176470588, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI37595754, Length=448, Percent_Identity=29.4642857142857, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI119964728, Length=464, Percent_Identity=27.1551724137931, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4501993, Length=255, Percent_Identity=23.5294117647059, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789351, Length=447, Percent_Identity=34.8993288590604, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1787977, Length=504, Percent_Identity=23.6111111111111, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994907, Length=446, Percent_Identity=23.7668161434978, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI48994914, Length=122, Percent_Identity=31.1475409836066, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=465, Percent_Identity=29.6774193548387, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71992381, Length=465, Percent_Identity=28.3870967741935, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71992392, Length=385, Percent_Identity=29.3506493506493, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17534361, Length=460, Percent_Identity=28.0434782608696, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17556096, Length=456, Percent_Identity=25.6578947368421, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320027, Length=427, Percent_Identity=28.3372365339578, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320023, Length=479, Percent_Identity=28.39248434238, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320764, Length=477, Percent_Identity=26.8343815513627, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI18921117, Length=469, Percent_Identity=26.6524520255864, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24639277, Length=469, Percent_Identity=26.6524520255864, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24639275, Length=469, Percent_Identity=26.6524520255864, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24653753, Length=467, Percent_Identity=23.1263383297645, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50555; Mature: 50555

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKRHLKYFKNLVGEEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
EHRLIVVPRGAGSGFTGGALSVSGGLVLSVEKYLDKILEIDTKNLIARVEPGVINKHFQN
CCCEEEEECCCCCCCCCCEEEECCCEEEEHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
EVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGITKDYVMALRVVLANGEVIRAG
HHHHEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEECH
KKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHF
HHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KANGCVGFKVAQNEQEEQDLWFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKI
HCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHH
SQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAMEEIFQAAINLEGTLSGEHGIG
HHHCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
LSKAKFMPLAFNDSEMELFRNIKKALDPNNILNPFKMGL
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MLEKRHLKYFKNLVGEEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
EHRLIVVPRGAGSGFTGGALSVSGGLVLSVEKYLDKILEIDTKNLIARVEPGVINKHFQN
CCCEEEEECCCCCCCCCCEEEECCCEEEEHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
EVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGITKDYVMALRVVLANGEVIRAG
HHHHEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEECH
KKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHF
HHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KANGCVGFKVAQNEQEEQDLWFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKI
HCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHH
SQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAMEEIFQAAINLEGTLSGEHGIG
HHHCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
LSKAKFMPLAFNDSEMELFRNIKKALDPNNILNPFKMGL
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]