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The map label for this gene is kgtP [H]

Identifier: 109947350

GI number: 109947350

Start: 683007

End: 684275

Strand: Reverse

Name: kgtP [H]

Synonym: Hac_0789

Alternate gene names: 109947350

Gene position: 684275-683007 (Counterclockwise)

Preceding gene: 109947351

Following gene: 109947349

Centisome position: 44.04

GC content: 38.38

Gene sequence:

>1269_bases
ATGAATCCTCAACAAGCCACCAAAAAACCTTTAAAATCTCTTCTAGCCGCTAGTTCAGGTAATTTAGTAGAATGGTATGA
TTTTTATGCTTATGCGTTCCTTGCTCCTTATTTTGCTAAGGACTTTACCCATACTAATGACCCTACTTTAGCGCTAATCT
CGGCTTTTTTGGTTTTCATGCTAGGGTTTTTCATGCGCCCTTTGGGGAGTTTGTTTTTTGGTAAATTGGGGGATAAAAAG
GGGCGTAAAACCTCTATGGTGTATTCTATTATCCTTATGGCACTAGGCTCTTTTATGTTGGCATTGCTCCCCACTAAAGA
AGTCGTAGGGGAATGGGCGTTCTTATTTTTATTATTAGCTAGGCTTTTACAAGGCTTTAGCGTGGGAGGTGAATATGGCG
TGGTCGCCACTTACCTCTCTGAATTAGGTAAGAATGGCAAAAAAGGTTTTTATGGCTCTTTTCAATATGTAACTCTAGTT
GGAGGGCAACTCTTAGCTATTTTTTCACTTTTTATTGTTGAAAACATTTATACGCATGAGCAAATCAGTGCATTTGCTTG
GCGTTATTTATTCGCTTTAGGGGGTATATTAGCCTTACTCTCGCTCTTTTTAAGAAACATCATGGAAGAGACTATGGGTA
GTGAAACAACTCCCCAAAAAGAAACCCAAAGGGGCAGTTTAAAGGAATTACTCAACCATAAAAAAGCCTTAATGATAGTC
TTTGGGATCACTATGGGAGGGAGTTTGTGTTTTTATACTTTTACGGTGTATTTAAAAATCTTTTTAACCAATAGCTCGTC
ATTTAGCCCTAAAGAAAGCAGTTTTATCATGCTTTTAGCGCTCACTTATTTTATCTTTTTACAGCCTTTATGCGGAATGC
TTGCAGATAGAATCAAACGCCAAAAAATGTTGATGATTTTTGCTATCACAGGGCTTATTGTAACTCCCCTTGTCTTTTAT
GGTATCAAACACGCCACTAGCGTGTATGAAGCCCTATTTTATGAAATGCTTGCATTGAGTACGATGAGCTTTTATACTTG
TATTGCTGGGGTTATTAAGGCGGAATTATTCCCTGAACATGTGCGAGCGCTTGGCGTGGGTCTAGCTTATGCGATTGCGA
ATGCGCTTTTTGGGGGGAGTGCTAGTTATGTAGCGTTAGAATTCAAACAACATGGTTTTGAAGTAGGGTTTGTGGGCTAT
GTCATGTTTAGTCTCATTATATACATGATTATGGTAATGCTATTCCCTAAAAAAACCTATTTGGAATAA

Upstream 100 bases:

>100_bases
ATAAATACCATAATCCTTTTATCTATTCGTTAAATGTTGTTGGTAGTTTTGATAACATCCTAGTAAAATGGTCTAAAAAT
TTCACATTCTAAGGATAACT

Downstream 100 bases:

>100_bases
ATTGGTTCTTATTTAATTTTTGCTATAATTTTTCCTTTTAAAAGGATTATTGCATGCAAAATGGGTATTATGCGGCCGCA
GGAGCTATGGCTACACAATT

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNPQQATKKPLKSLLAASSGNLVEWYDFYAYAFLAPYFAKDFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKK
GRKTSMVYSIILMALGSFMLALLPTKEVVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVTLV
GGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMGSETTPQKETQRGSLKELLNHKKALMIV
FGITMGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLALTYFIFLQPLCGMLADRIKRQKMLMIFAITGLIVTPLVFY
GIKHATSVYEALFYEMLALSTMSFYTCIAGVIKAELFPEHVRALGVGLAYAIANALFGGSASYVALEFKQHGFEVGFVGY
VMFSLIIYMIMVMLFPKKTYLE

Sequences:

>Translated_422_residues
MNPQQATKKPLKSLLAASSGNLVEWYDFYAYAFLAPYFAKDFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKK
GRKTSMVYSIILMALGSFMLALLPTKEVVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVTLV
GGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMGSETTPQKETQRGSLKELLNHKKALMIV
FGITMGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLALTYFIFLQPLCGMLADRIKRQKMLMIFAITGLIVTPLVFY
GIKHATSVYEALFYEMLALSTMSFYTCIAGVIKAELFPEHVRALGVGLAYAIANALFGGSASYVALEFKQHGFEVGFVGY
VMFSLIIYMIMVMLFPKKTYLE
>Mature_422_residues
MNPQQATKKPLKSLLAASSGNLVEWYDFYAYAFLAPYFAKDFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKK
GRKTSMVYSIILMALGSFMLALLPTKEVVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVTLV
GGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMGSETTPQKETQRGSLKELLNHKKALMIV
FGITMGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLALTYFIFLQPLCGMLADRIKRQKMLMIFAITGLIVTPLVFY
GIKHATSVYEALFYEMLALSTMSFYTCIAGVIKAELFPEHVRALGVGLAYAIANALFGGSASYVALEFKQHGFEVGFVGY
VMFSLIIYMIMVMLFPKKTYLE

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=412, Percent_Identity=46.1165048543689, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1790550, Length=376, Percent_Identity=28.7234042553192, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788292, Length=396, Percent_Identity=28.5353535353535, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1789941, Length=402, Percent_Identity=30.8457711442786, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082404, Length=438, Percent_Identity=25.1141552511416, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082231, Length=234, Percent_Identity=26.0683760683761, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47174; Mature: 47174

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]