Click here to switch to the map view.

The map label for this gene is 109947236

Identifier: 109947236

GI number: 109947236

Start: 579629

End: 580510

Strand: Reverse

Name: 109947236

Synonym: Hac_0661

Alternate gene names: NA

Gene position: 580510-579629 (Counterclockwise)

Preceding gene: 109947237

Following gene: 109947235

Centisome position: 37.36

GC content: 40.82

Gene sequence:

>882_bases
ATGATTTGTGCGAGCATTCTCCAACACGCTTATTGCGGCTCTAGAAAAAAGACTATAGAACATACAGCACACTTGCTTGA
ACAAGCACTCAAAAAACACCCTAAAACCAATTTAGTGGTGTTGCAAGAATTAAACCCTTATAGTTATTTTTGCCAAAGCG
AAAACCCTAAATTTTTTGATTTAGGCGAGTATTTTGAAGAAGATAAGGCTTTTTTTAGCGCTTTAGCTCAAAAGTTCAAG
GTGGTGCTTGTCGCTTCTTTATTTGAAAAGCGCGCTAAAGGGTTGTATCACAATAGTGCGGTTGTGTTTGAAAAAGACGG
CTCAATCGTTGGAATTTATCGCAAAATGCACATCCCTGATGATCCGGGGTTTTATGAAAAATTTTATTTCACGCCGGGGG
ATTTGGGTTTTGAGCCTATTGTCACAAGCGTGGGGAAATTAGGGCTTATGGTGTGTTGGGATCAATGGTATCCTGAAGCG
GCTAGGATTATGGCTTTAAAAGGGGCGGAGATTTTAATCTATCCTAGTGCGATAGGGTTTTTAGAAGAAGATTCCAATGA
AGAAAAAAAACGCCAGCAAAACGCATGGGAGACGATCCAAAGAGGGCATGCGATCGCTAATGGCTTGCCTTTGATTGCGA
CTAACAGAGTGGGTGTGGAGCTGGATCCTAGTGGCGTAATTAAGGGGGGTATTACTTTTTTTGGCTCTAGTTTTGTGGTG
GGGGCTTTAGGCGAGTTTTTAGCTAAAGCGAGCGATAAAGAAGAAATTTTGTATGCAGAAATTGATTTAGGACACACAGA
AGAAGTGCGCAGAATGTGGCCGTTTTTAAGAGACAGACGCATTGATTTTTATCATGATTTGTTGAAACGCTATAAAGATT
GA

Upstream 100 bases:

>100_bases
GAGCTTTGAAGAATATGATGAGGGGTATGATGATTATAAGAATGTCTATGATGATGACGATTATCAAGATTATAGCTCTG
ATTATGAAGAAGAGTGAAAA

Downstream 100 bases:

>100_bases
TTTTAGAAAGGATTGGCATGGCAAGTGCAGTTTTTTGTGCTAAAAAAGCGCAAAAATTCTATCATTTTTGCGTGGTATTT
TCACTTTAACAAGGAGCAAA

Product: beta-alanine synthetase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MICASILQHAYCGSRKKTIEHTAHLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKAFFSALAQKFK
VVLVASLFEKRAKGLYHNSAVVFEKDGSIVGIYRKMHIPDDPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEA
ARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGVIKGGITFFGSSFVV
GALGEFLAKASDKEEILYAEIDLGHTEEVRRMWPFLRDRRIDFYHDLLKRYKD

Sequences:

>Translated_293_residues
MICASILQHAYCGSRKKTIEHTAHLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKAFFSALAQKFK
VVLVASLFEKRAKGLYHNSAVVFEKDGSIVGIYRKMHIPDDPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEA
ARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGVIKGGITFFGSSFVV
GALGEFLAKASDKEEILYAEIDLGHTEEVRRMWPFLRDRRIDFYHDLLKRYKD
>Mature_293_residues
MICASILQHAYCGSRKKTIEHTAHLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKAFFSALAQKFK
VVLVASLFEKRAKGLYHNSAVVFEKDGSIVGIYRKMHIPDDPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEA
ARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGVIKGGITFFGSSFVV
GALGEFLAKASDKEEILYAEIDLGHTEEVRRMWPFLRDRRIDFYHDLLKRYKD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=229, Percent_Identity=31.0043668122271, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI5031947, Length=236, Percent_Identity=29.2372881355932, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI297632350, Length=236, Percent_Identity=29.2372881355932, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI297632348, Length=236, Percent_Identity=29.2372881355932, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI7706509, Length=265, Percent_Identity=28.6792452830189, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533173, Length=264, Percent_Identity=30.3030303030303, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17556280, Length=293, Percent_Identity=26.2798634812287, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=285, Percent_Identity=30.1754385964912, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322335, Length=207, Percent_Identity=25.1207729468599, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358471, Length=270, Percent_Identity=30, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355835, Length=285, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17933642, Length=220, Percent_Identity=26.8181818181818, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33405; Mature: 33405

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICASILQHAYCGSRKKTIEHTAHLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFD
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEE
LGEYFEEDKAFFSALAQKFKVVLVASLFEKRAKGLYHNSAVVFEKDGSIVGIYRKMHIPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEECCCC
DPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEAARIMALKGAEILIYPSAIGF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEEHHCCCCHHHEEEECCCEEEEEECHHCC
LEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGVIKGGITFFGSSFVV
CCCCCCHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCEEECCCCCEECCHHHHHHHHHH
GALGEFLAKASDKEEILYAEIDLGHTEEVRRMWPFLRDRRIDFYHDLLKRYKD
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MICASILQHAYCGSRKKTIEHTAHLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFD
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEE
LGEYFEEDKAFFSALAQKFKVVLVASLFEKRAKGLYHNSAVVFEKDGSIVGIYRKMHIPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEECCCC
DPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEAARIMALKGAEILIYPSAIGF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEEHHCCCCHHHEEEECCCEEEEEECHHCC
LEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGVIKGGITFFGSSFVV
CCCCCCHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCEEECCCCCEECCHHHHHHHHHH
GALGEFLAKASDKEEILYAEIDLGHTEEVRRMWPFLRDRRIDFYHDLLKRYKD
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]