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The map label for this gene is ispH [H]

Identifier: 109947060

GI number: 109947060

Start: 406005

End: 406817

Strand: Reverse

Name: ispH [H]

Synonym: Hac_0458

Alternate gene names: 109947060

Gene position: 406817-406005 (Counterclockwise)

Preceding gene: 109947061

Following gene: 109947059

Centisome position: 26.18

GC content: 38.62

Gene sequence:

>813_bases
ATGGCTAAGGATTATGGTTTTTGTTTTGGTGTCAAAAGAGCGATACAGATCGCTGAAAAAAATCAAAATAGCCTGATTTT
TGGCTCGCTCATCCATAACGCTAAAGAAATCAATCGTTTGGAAAAAAACTTCAATGTGAAAATTGAAGAAGATCCTAAAA
AAATCCCTAAAGACAAGAGCGTGATCATAAGAACCCATGGCATTCCCAAGCAAGATTTAGAATATTTGAAAAATAAGGGG
GTAAAAATCACTGATGCGACCTGTCCGTATGTGATCAAACCCCAACAAATCGTAGAATCCATGAGTAAAGAGGGGTATCA
GATCGTGCTTTTTGGGGATATTAACCACCCTGAAGTCAAGGGCGTGATTAGCTATGCCACTAATCAAGCTTTAGTAGTCA
ATTCTTTAGAAAAATTGCAAGAAAAAAAGCTCCAACGAAAAGTGGCTCTAGTCTCTCAAACCACCAAACAAACCCCAAAA
CTCTTACAAATCGCTTCTTATTTGGTTGAAAAATGCACTGAAGTGCGTATTTTTAACACGATCTGCAACGCCACTTCCTA
CAACCAAAAAGCCGCTTTGGATTTGAGTAAAGAAGTGGATATTATGATAGTCGTGGGCGGTAAAACTTCTTCAAACACCA
AACAGCTCTTAAGCATCGCCAAACAGCATTGTAAAGACAGCTACTTGGTAGAAGATGAAAACGAATTGGAAATGGCGTGG
TTTAAGGATAAAAAATTGTGCGGAATTACCGCTGGGGCTTCCACGCCGGATTGGATTATAGAAAATGTCAAGCAAAAAAT
TAGCACGATTTAA

Upstream 100 bases:

>100_bases
CCTTTAGAAATTGATAATTTAGAATGTGCAAACATTTCTTTCCCGCAATTCAAGCGTTTGCTCAATTTATTTAAAAAAGG
GAGTCTCCATGGAAATTAAA

Downstream 100 bases:

>100_bases
CACATTTTGAAATTTTTCTTTAAAAAAACAGCTAATTGTTGGTTTTTAAACGATTTTGTAAGTCAAAAAAAGATACAATG
ATCTTAAGGATTTTATATTT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKDKSVIIRTHGIPKQDLEYLKNKG
VKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVVNSLEKLQEKKLQRKVALVSQTTKQTPK
LLQIASYLVEKCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENELEMAW
FKDKKLCGITAGASTPDWIIENVKQKISTI

Sequences:

>Translated_270_residues
MAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKDKSVIIRTHGIPKQDLEYLKNKG
VKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVVNSLEKLQEKKLQRKVALVSQTTKQTPK
LLQIASYLVEKCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENELEMAW
FKDKKLCGITAGASTPDWIIENVKQKISTI
>Mature_269_residues
AKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKDKSVIIRTHGIPKQDLEYLKNKGV
KITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVVNSLEKLQEKKLQRKVALVSQTTKQTPKL
LQIASYLVEKCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENELEMAWF
KDKKLCGITAGASTPDWIIENVKQKISTI

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=282, Percent_Identity=29.0780141843972, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 30406; Mature: 30274

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKDKS
CCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCE
VIIRTHGIPKQDLEYLKNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVK
EEEEECCCCHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCCCHHH
GVISYATNQALVVNSLEKLQEKKLQRKVALVSQTTKQTPKLLQIASYLVEKCTEVRIFNT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENELEMAW
HHCCCCCCCHHHEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCEEEEE
FKDKKLCGITAGASTPDWIIENVKQKISTI
ECCCCEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKDKS
CCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCE
VIIRTHGIPKQDLEYLKNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVK
EEEEECCCCHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCCCHHH
GVISYATNQALVVNSLEKLQEKKLQRKVALVSQTTKQTPKLLQIASYLVEKCTEVRIFNT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENELEMAW
HHCCCCCCCHHHEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCEEEEE
FKDKKLCGITAGASTPDWIIENVKQKISTI
ECCCCEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA