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The map label for this gene is truA

Identifier: 109947020

GI number: 109947020

Start: 360514

End: 361242

Strand: Direct

Name: truA

Synonym: Hac_0414

Alternate gene names: 109947020

Gene position: 360514-361242 (Clockwise)

Preceding gene: 109947019

Following gene: 109947023

Centisome position: 23.2

GC content: 39.37

Gene sequence:

>729_bases
ATGCGTTGTTTTAAGGCTACTATCGCTTATGATGGGGCGTATTTTTTAGGCTATGCCAAACAGCCTGACAAACTTGGCGT
GCAAGATAAAATTGAAAGCGCTCTAAATTTGCTAGGGATTAAAAGCGTAGTGATTGCAGCCGGTCGCACCGATAAAGGCG
TGCATGCAAACAATCAAGTGCTATCTTTTCATGCTCAAAAACACTGGAGTGCTGATAAGTTGTTTTATTATCTAGCCCCT
AAACTCGCTCCGCATATTGTCTTAAAAAAATTAGAAGAAAAAAATTTCCATGCTCGTTTTGACGCTCAAAAAAGAGCGTA
TCGCTACCTTTTAACTAAAAGTTTAAAAACACCTTTTTTAGCACCTTATATCGCTTGTGGGGATTATGGATCATTAGATT
CATTAAACACCGCTTTAAAGCAATTTATAGGCAAGCATGATTTTTCCATGTTTAAAAAAGAAGGCGGGGCGGCAACCAAC
CCCAAACGCATCATTTTTAACGCGTTTGCTTATAAGGCTTCTATCATGGGGCATGAGTGCGTAGTGTTTAAAATTATCGG
CGATGCGTTTTTGCGCTCTAGCGTGCGTTTGATCATGCAAGCATGCGTTCAATACTCCTTAGAAAAAATCACGCTCGCTG
AAATTAAAACACAAATCAATAACATCAAAGCCACCATAAGAACGCCCATAATGGCTAACGGATTGTATTTACATAGGGTG
CATTATTGA

Upstream 100 bases:

>100_bases
ATTTCTACCGATTTGTTTTTGATGACCTTTATCTTCCCCTTTATTTGGGCGTTTGCCTCTTATTTGTTGTTTAGAAAATT
CATTTTAAAGCGTTATTAAA

Downstream 100 bases:

>100_bases
AATCTTAAAAGGTGTTCTTCCCAAGCCAAAGCGCTTTTGATGATGGTGTCTAGGTTGTTATAAAGGGGTTTGAAAGAGGT
GTTTTGTAAGATTTTAGCGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRCFKATIAYDGAYFLGYAKQPDKLGVQDKIESALNLLGIKSVVIAAGRTDKGVHANNQVLSFHAQKHWSADKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKSLKTPFLAPYIACGDYGSLDSLNTALKQFIGKHDFSMFKKEGGAATN
PKRIIFNAFAYKASIMGHECVVFKIIGDAFLRSSVRLIMQACVQYSLEKITLAEIKTQINNIKATIRTPIMANGLYLHRV
HY

Sequences:

>Translated_242_residues
MRCFKATIAYDGAYFLGYAKQPDKLGVQDKIESALNLLGIKSVVIAAGRTDKGVHANNQVLSFHAQKHWSADKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKSLKTPFLAPYIACGDYGSLDSLNTALKQFIGKHDFSMFKKEGGAATN
PKRIIFNAFAYKASIMGHECVVFKIIGDAFLRSSVRLIMQACVQYSLEKITLAEIKTQINNIKATIRTPIMANGLYLHRV
HY
>Mature_242_residues
MRCFKATIAYDGAYFLGYAKQPDKLGVQDKIESALNLLGIKSVVIAAGRTDKGVHANNQVLSFHAQKHWSADKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKSLKTPFLAPYIACGDYGSLDSLNTALKQFIGKHDFSMFKKEGGAATN
PKRIIFNAFAYKASIMGHECVVFKIIGDAFLRSSVRLIMQACVQYSLEKITLAEIKTQINNIKATIRTPIMANGLYLHRV
HY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Escherichia coli, GI1788657, Length=244, Percent_Identity=27.4590163934426, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_HELAH (Q17YM7)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664248.1
- ProteinModelPortal:   Q17YM7
- SMR:   Q17YM7
- STRING:   Q17YM7
- GeneID:   4176670
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0414
- NMPDR:   fig|382638.8.peg.403
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   KGVHANN
- ProtClustDB:   PRK00021
- BioCyc:   HACI382638:HAC_0414-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27285; Mature: 27285

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCFKATIAYDGAYFLGYAKQPDKLGVQDKIESALNLLGIKSVVIAAGRTDKGVHANNQV
CCEEEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCE
LSFHAQKHWSADKLFYYLAPKLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKSLKTPFL
EEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHCCCCCC
APYIACGDYGSLDSLNTALKQFIGKHDFSMFKKEGGAATNPKRIIFNAFAYKASIMGHEC
CCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHCCCCE
VVFKIIGDAFLRSSVRLIMQACVQYSLEKITLAEIKTQINNIKATIRTPIMANGLYLHRV
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCEEEEEE
HY
CC
>Mature Secondary Structure
MRCFKATIAYDGAYFLGYAKQPDKLGVQDKIESALNLLGIKSVVIAAGRTDKGVHANNQV
CCEEEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCE
LSFHAQKHWSADKLFYYLAPKLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKSLKTPFL
EEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHCCCCCC
APYIACGDYGSLDSLNTALKQFIGKHDFSMFKKEGGAATNPKRIIFNAFAYKASIMGHEC
CCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHCCCCE
VVFKIIGDAFLRSSVRLIMQACVQYSLEKITLAEIKTQINNIKATIRTPIMANGLYLHRV
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCEEEEEE
HY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA