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The map label for this gene is metK

Identifier: 109946990

GI number: 109946990

Start: 328425

End: 329582

Strand: Direct

Name: metK

Synonym: Hac_0382

Alternate gene names: 109946990

Gene position: 328425-329582 (Clockwise)

Preceding gene: 109946989

Following gene: 109946991

Centisome position: 21.14

GC content: 42.49

Gene sequence:

>1158_bases
ATGAAAGATAGTTTTCTTTTTACTTCAGAATCAGTAACCGAAGGGCATCCTGATAAAATGGCCGATCAAATCAGCGATGC
GGTTTTAGATTATATTATTGAGCGGGATAAAAAAGCTAAAGTCGCATGCGAGACTTTGGTTTCTAATGGTTTTTGCGTGA
TCACTGGCGAGTTAAAAACTTCTATCTATGCTCCTATGCAAGAGATTGCAAGAGAAGTGGTTAAAAAAATTGGTTATACA
GACGCTCTTTATGGCTTTGATTATAGAAGCGCGGCGGTTTTAAATGGCATTGGTGAGCAAAGCCCTGATATTAATCAAGG
CGTGGATAGAGAAGATGGCGAGATTGGGGCAGGGGATCAAGGGCTTGTGTTTGGTTATGCGTGCAAAGAGACTCAAATGC
TCATGCCTTTACCCATTCATTTAGCGCACCAACTCACTTTCGCTCTAGCTCAAAAAAGAAAAGACAACACCTTGCCTTTT
TTAAGGCCTGATGGCAAGTCTCAAGTGAGCGTTCGCTATGAGAATAACAAACCCATAAGCATTGATACGATCGTCATTTC
TACCCAACATTCCCCAGAAGTTTCACAAAAACATTTAAAAGAAGCGGTGATTGAAGAGATCGTGTATAAGGTTTTACCCA
AAGAATATTTGCATGACAATATCAAGTTTTTTGTCAATCCCACAGGAAAATTCGTTATCGGTGGACCACAAGGTGATGCG
GGTTTGACAGGAAGAAAAATCATCGTGGATACTTATGGGGGGAGTTGTCCGCATGGGGGTGGAGCGTTTAGCGGGAAAGA
CCCTAGTAAGGTGGATAGGAGTGCAGCTTATGCGGCCCGCTATGTGGCTAAAAATTTGGTAGCGAGTGGGGTTTGCGATA
GAGCGACCGTGCAGCTTGCTTATGCGATTGGGGTTGTAGAGCCGGTGTCCATTTATGTGAATACGCATAACACGAGCAAG
TATTCAAGCGCGGAATTAGAAAAATGCGTGAAATTGGTTTTTAAACTCACGCCAAAAGGCATTATTGAAAGCTTGGATTT
GTTAAGACCTATTTATTCGCTCACTTCATCTTATGGGCATTTTGGGCGCGAGTTGGAGGCATTCACTTGGGAAAAGACCA
ACAAGGCTGAAGAGATTAAAGCGTTCTTTAAGCATTAA

Upstream 100 bases:

>100_bases
GAGTTTGTTGCAAAAAGCTAAAGGGTTTTTTAAGTCTTAAAGAATGAAATAAGCTATAATAAGCCCATTTTGAATACAAA
CTTTATCACAAGGACAATCA

Downstream 100 bases:

>100_bases
AAAAATATTTTAAGGGTCATATTTTAAAAAATTTTTGTATAATCAACAATTCACAAGGAGTTTAAATTGAAACAAAGAAC
GCTGTCTATTATTAAACCGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDKKAKVACETLVSNGFCVITGELKTSIYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQGLVFGYACKETQMLMPLPIHLAHQLTFALAQKRKDNTLPF
LRPDGKSQVSVRYENNKPISIDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDRATVQLAYAIGVVEPVSIYVNTHNTSK
YSSAELEKCVKLVFKLTPKGIIESLDLLRPIYSLTSSYGHFGRELEAFTWEKTNKAEEIKAFFKH

Sequences:

>Translated_385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDKKAKVACETLVSNGFCVITGELKTSIYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQGLVFGYACKETQMLMPLPIHLAHQLTFALAQKRKDNTLPF
LRPDGKSQVSVRYENNKPISIDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDRATVQLAYAIGVVEPVSIYVNTHNTSK
YSSAELEKCVKLVFKLTPKGIIESLDLLRPIYSLTSSYGHFGRELEAFTWEKTNKAEEIKAFFKH
>Mature_385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDKKAKVACETLVSNGFCVITGELKTSIYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQGLVFGYACKETQMLMPLPIHLAHQLTFALAQKRKDNTLPF
LRPDGKSQVSVRYENNKPISIDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDRATVQLAYAIGVVEPVSIYVNTHNTSK
YSSAELEKCVKLVFKLTPKGIIESLDLLRPIYSLTSSYGHFGRELEAFTWEKTNKAEEIKAFFKH

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=54.4973544973545, Blast_Score=390, Evalue=1e-109,
Organism=Homo sapiens, GI4557737, Length=377, Percent_Identity=51.7241379310345, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=61.968085106383, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=51.0582010582011, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=51.0526315789474, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=51.6042780748663, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=51.3368983957219, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=47.5935828877005, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25145635, Length=329, Percent_Identity=48.6322188449848, Blast_Score=304, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=48.7730061349693, Blast_Score=301, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=50.1661129568106, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=54.8780487804878, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=53.9473684210526, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=52.6455026455027, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=50.7894736842105, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=50.7894736842105, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=51.0526315789474, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=51.0526315789474, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=51.0526315789474, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=51.0526315789474, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=51.0526315789474, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=51.0526315789474, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580541, Length=366, Percent_Identity=49.4535519125683, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=50.4201680672269, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_HELAH (Q17YQ7)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664218.1
- ProteinModelPortal:   Q17YQ7
- SMR:   Q17YQ7
- STRING:   Q17YQ7
- GeneID:   4176762
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0382
- NMPDR:   fig|382638.8.peg.371
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q17YQ7
- ProtClustDB:   PRK05250
- BioCyc:   HACI382638:HAC_0382-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42359; Mature: 42359

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDKKAKVACETLVSNGFCVITGELKT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCHHH
SIYAPMQEIAREVVKKIGYTDALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQ
HHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GLVFGYACKETQMLMPLPIHLAHQLTFALAQKRKDNTLPFLRPDGKSQVSVRYENNKPIS
CEEEEEECCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCEE
IDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEECCCCCC
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDRATVQLA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YAIGVVEPVSIYVNTHNTSKYSSAELEKCVKLVFKLTPKGIIESLDLLRPIYSLTSSYGH
HHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FGRELEAFTWEKTNKAEEIKAFFKH
HCCHHHHEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDKKAKVACETLVSNGFCVITGELKT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCHHH
SIYAPMQEIAREVVKKIGYTDALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQ
HHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GLVFGYACKETQMLMPLPIHLAHQLTFALAQKRKDNTLPFLRPDGKSQVSVRYENNKPIS
CEEEEEECCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCEE
IDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEECCCCCC
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDRATVQLA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YAIGVVEPVSIYVNTHNTSKYSSAELEKCVKLVFKLTPKGIIESLDLLRPIYSLTSSYGH
HHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FGRELEAFTWEKTNKAEEIKAFFKH
HCCHHHHEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA