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The map label for this gene is tpiA

Identifier: 109946987

GI number: 109946987

Start: 325802

End: 326506

Strand: Direct

Name: tpiA

Synonym: Hac_0379

Alternate gene names: 109946987

Gene position: 325802-326506 (Clockwise)

Preceding gene: 109946982

Following gene: 109946988

Centisome position: 20.97

GC content: 33.33

Gene sequence:

>705_bases
ATGACAAAAATTGCAATGGCTAATTTTAAATCCGCTATGCCTATTTTTAAAAGCCATGCGTATTTGAAAGAATTGGAAAA
AACTTTAAAGCCGCAGCATTGTGATAGGGTGTTTGTATTCCCTGATTTTTTGGGGTTATTGCCTAATGCGTTTTTGCATT
TCACTTTAGGAGTGCAAAACGCTTACCCTAAAGATTGTGGGGCTTTTACGGGTGAAATCACTTCAAAGCATTTAGAAGAA
TTGAAAATCAACACGCTTTTAATAGGGCATAGCGAGAGACGAGTGCTTTTAAAGGAAAGCCCTAACTTTTTGAAAGAAAA
GTTTGATTTTTTTAAAGATAAAAAATTTAAAATCGTCTATTGTATTGGCGAAGATTTAAAAACTAGAGAAAAGGGTTTAG
GAGCTGTAAAAGAATTTTTAAATGAACAATTAGAAAACATTGATCTTGATTATCAAAATTTAATCGTGGCTTATGAGCCT
ATTTGGGCGATTGGCACAGGAAAAAGCGCTTCTTTAGAAGATATTTATCTCACGCATGGTTTTTTAAAGCAACATTTAAA
TCAAAAAATGCCCTTATTGTATGGGGGGAGCGTGAATACACAAAACGCTAAAGAAATTTTAGGGATTGATAGCGTGGATG
GCTTATTGATTGGGAGCACGTCTTTGGAATTAGAAAATTTTAAAACAATCATTTCATTTTTGTAA

Upstream 100 bases:

>100_bases
AAAAATTAAATTCAAGGGGTCTATTGTTTAAAATTTACATTTTCTAAACGCTTTGGATATAATAAGAGCTAGTTACCACA
TATAAAAATAAGGATCTTTG

Downstream 100 bases:

>100_bases
AGGAAAATTATGGGATTTTTAAAAGGTAAAAAAGGGCTTATTGTAGGGGTTGCGAATAATAAATCCATCGCTTGTGGGAT
CGCTCAATCTTGTTTTAATC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MTKIAMANFKSAMPIFKSHAYLKELEKTLKPQHCDRVFVFPDFLGLLPNAFLHFTLGVQNAYPKDCGAFTGEITSKHLEE
LKINTLLIGHSERRVLLKESPNFLKEKFDFFKDKKFKIVYCIGEDLKTREKGLGAVKEFLNEQLENIDLDYQNLIVAYEP
IWAIGTGKSASLEDIYLTHGFLKQHLNQKMPLLYGGSVNTQNAKEILGIDSVDGLLIGSTSLELENFKTIISFL

Sequences:

>Translated_234_residues
MTKIAMANFKSAMPIFKSHAYLKELEKTLKPQHCDRVFVFPDFLGLLPNAFLHFTLGVQNAYPKDCGAFTGEITSKHLEE
LKINTLLIGHSERRVLLKESPNFLKEKFDFFKDKKFKIVYCIGEDLKTREKGLGAVKEFLNEQLENIDLDYQNLIVAYEP
IWAIGTGKSASLEDIYLTHGFLKQHLNQKMPLLYGGSVNTQNAKEILGIDSVDGLLIGSTSLELENFKTIISFL
>Mature_233_residues
TKIAMANFKSAMPIFKSHAYLKELEKTLKPQHCDRVFVFPDFLGLLPNAFLHFTLGVQNAYPKDCGAFTGEITSKHLEEL
KINTLLIGHSERRVLLKESPNFLKEKFDFFKDKKFKIVYCIGEDLKTREKGLGAVKEFLNEQLENIDLDYQNLIVAYEPI
WAIGTGKSASLEDIYLTHGFLKQHLNQKMPLLYGGSVNTQNAKEILGIDSVDGLLIGSTSLELENFKTIISFL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=198, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI226529917, Length=198, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1790353, Length=191, Percent_Identity=35.0785340314136, Blast_Score=127, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17536593, Length=186, Percent_Identity=37.0967741935484, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320255, Length=183, Percent_Identity=38.2513661202186, Blast_Score=125, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28572008, Length=222, Percent_Identity=35.5855855855856, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28572006, Length=222, Percent_Identity=35.5855855855856, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28572004, Length=222, Percent_Identity=35.5855855855856, Blast_Score=128, Evalue=3e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_HELAH (Q17YR0)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664215.1
- ProteinModelPortal:   Q17YR0
- SMR:   Q17YR0
- STRING:   Q17YR0
- GeneID:   4177480
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0379
- NMPDR:   fig|382638.8.peg.368
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   IEKNGTM
- ProtClustDB:   PRK00042
- BioCyc:   HACI382638:HAC_0379-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26551; Mature: 26420

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 90-90 ACT_SITE 159-159 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAMANFKSAMPIFKSHAYLKELEKTLKPQHCDRVFVFPDFLGLLPNAFLHFTLGVQN
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHEEEEECCCC
AYPKDCGAFTGEITSKHLEELKINTLLIGHSERRVLLKESPNFLKEKFDFFKDKKFKIVY
CCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEE
CIGEDLKTREKGLGAVKEFLNEQLENIDLDYQNLIVAYEPIWAIGTGKSASLEDIYLTHG
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCEEEEECCCCCCCHHHHHHHH
FLKQHLNQKMPLLYGGSVNTQNAKEILGIDSVDGLLIGSTSLELENFKTIISFL
HHHHHHCCCCCEEECCCCCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TKIAMANFKSAMPIFKSHAYLKELEKTLKPQHCDRVFVFPDFLGLLPNAFLHFTLGVQN
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHEEEEECCCC
AYPKDCGAFTGEITSKHLEELKINTLLIGHSERRVLLKESPNFLKEKFDFFKDKKFKIVY
CCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEE
CIGEDLKTREKGLGAVKEFLNEQLENIDLDYQNLIVAYEPIWAIGTGKSASLEDIYLTHG
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCEEEEECCCCCCCHHHHHHHH
FLKQHLNQKMPLLYGGSVNTQNAKEILGIDSVDGLLIGSTSLELENFKTIISFL
HHHHHHCCCCCEEECCCCCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA