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The map label for this gene is frdA [H]

Identifier: 109946985

GI number: 109946985

Start: 322667

End: 324811

Strand: Reverse

Name: frdA [H]

Synonym: Hac_0377

Alternate gene names: 109946985

Gene position: 324811-322667 (Counterclockwise)

Preceding gene: 109946986

Following gene: 109946984

Centisome position: 20.9

GC content: 44.34

Gene sequence:

>2145_bases
ATGAAAATAACATATTGTGATGCGTTGATTATTGGAGGCGGACTAGCCGGCTTAAGGGCTAGTATTGCATGCAAACAAAA
GGGTTTAAACACCATTGTTTTGAGTTTAGTGCCTGTTAGGCGTTCGCACTCTGCGGCCGCTCAAGGGGGCATGCAAGCAA
GCCTAGCGAACGCTAAAAAAAGCGAGGGCGATAACGAAGATTTGCACTTTTTAGATACGGTTAAGGGGAGCGATTGGGGG
TGCGATCAACAAGTGGCTAGAATGTTTGTAACCACTGCCCCTAAAGCCATTAGGGAATTAGCCAGTTGGGGGGTGCCTTG
GACTAGGATCAAAAAAGGCGATAGGTCTGCAGTCGTTAATGGTGAGCATGTCACTATCACTGAAAGAGACGATAGGCATG
GCTATATTTTAAGCCGTGATTTTGGAGGCACTAAAAAATGGCGCACATGCTTTACCGCTGATGCCACAGGGCATACCATG
CTTTATGCGGTCGCCAATGAAGCCTTGCACCACAAGGTGGATATTCAAGACAGAAAAGACATGCTCGCTTTCATCCATCA
TGATAATAAGTGCTATGGGGCGGTGGTAAGGGATTTGATTACCGGCGAAATTTCAGCGTATGTTTCTAAAGGCACGCTTC
TAGCTACTGGGGGTTATGGGCGCGTGTATAAACACACCACTAACGCGGTGATTTGCGATGGAGCTGGGGCTGCAAGTGCT
TTAGAAACCGGCGTGGCTAAATTAGGGAATATGGAAGCGGTGCAATTCCACCCTACCGCTTTAGTGCCGAGTGGGATTTT
AATGACCGAAGGTTGTAGGGGCGATGGAGGGGTTTTGAGAGACAAGTTTGGAAGACGCTTCATGCCCGCTTATGAGCCGG
AGAAAAAAGAGCTTGCAAGTAGAGACGTGGTCTCAAGGCGGATTTTAGAGCATATCCAAAAAGGTTATGGGGCTAAATCG
CCTTATGGAGACCATGTGTGGTTGGATATTGCTATTTTAGGGCGTAGTCATGTGGAAAAAAATTTAAGAGATGTGCGCGA
TATTGCCATGACTTTTGCCGGCATTGATCCGGCTGATAGCGAAGAGCAAACTAAAGACAACATGCAAGGCATGCCCGCCA
ATGAGTCCGAATACGGACAAGCGATGGCTAAACAAAAAGGCTGGATCCCCATAAAACCCATGCAACACTATTCTATGGGT
GGGGTTAGGACAAACCCTAAAGGCGAAACCCATTTGAAAGGTCTGTTTTGTGCGGGCGAAGCGGCATGCTGGGATTTGCA
TGGGTTTAACCGCTTGGGGGGTAATTCTGTGAGTGAAGCCGTGGTGGCTGGCATGATTATAGGGGATTATTTCGCCTCGC
ATTGTTTGGAAGCGCAAATTGAAATCAACACGCAAAAAATTGAAGCTTTCATTAAAGAAAGCCAGAATTACATGCACTTT
TTATTACACAATGAAGGCGATGAAGATGTGTATGAAATTAGAGAACGCATGAAAGAAGTCATGGATGAAAAAGTGGGCGT
TTTTAGAGAAGGCAAAAGATTAGAAGAAGCTCTTAAAGAATTGCAAGAGCTTTATGCACGCTCCAAAAACATTTGCGTGA
AAAACAAGGTTTTACACAATAACCCTGAATTAGAAGACGCTTATCGCACCAAAAAAATGCTCAAACTCGCACTTTGTATC
ACTCAAGGGGCGTTATTACGCACTGAAAGCAGAGGGGCTCACACAAGGATTGATTACCCTAAAAGAGACGATGAAAAATG
GCTTAATCGGACTTTAGCGAGTTGGCCTAATGCTGAACAAGACATGCCCACGATTGAATATGAAGAATTAGATGTGATGA
AAATGGAAATTAGCCCTGATTTTAGGGGCTATGGCAAAAAAGGTAATTTCATTCCCCACCCCAAAAAAGAAGAGCGCGAT
GCTGAAATTTTAAAAACGATTTTAGAATTAGAAAAGCTTGGAAAAGACAGAGTAGAAGTCCAGCATGCACTCATGCCTTT
TGAATTGCAAGAAAAATACAAAGCTAGGAATATGCGTTTAGAAGATGAAGAGGTGAGAGCTAGGGGGGAACATTTGTATT
CTTTCAATGTCCATGATTTATTAGACAAACACAACGCCAATCTAAAAGGAGAACACCATGGGTGA

Upstream 100 bases:

>100_bases
TTATATCAAAAAAGGTTTGGAAAACAAAGATAATGGCATTAAAACCATGCAAGAAGCCATAGAGACTGATGGAAAATTCC
ACAAAGAATAAGGGTAGAAA

Downstream 100 bases:

>100_bases
TAATGAACGAACGATTATAGTTAGAGTGCTAAAGTTTGACCCTCAAAGCACGGTGAGTAAGCCGCATTTTAAAGAGTATC
AGTTGAAAGAAACGCCATCC

Product: fumarate reductase flavoprotein subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKITYCDALIIGGGLAGLRASIACKQKGLNTIVLSLVPVRRSHSAAAQGGMQASLANAKKSEGDNEDLHFLDTVKGSDWG
CDQQVARMFVTTAPKAIRELASWGVPWTRIKKGDRSAVVNGEHVTITERDDRHGYILSRDFGGTKKWRTCFTADATGHTM
LYAVANEALHHKVDIQDRKDMLAFIHHDNKCYGAVVRDLITGEISAYVSKGTLLATGGYGRVYKHTTNAVICDGAGAASA
LETGVAKLGNMEAVQFHPTALVPSGILMTEGCRGDGGVLRDKFGRRFMPAYEPEKKELASRDVVSRRILEHIQKGYGAKS
PYGDHVWLDIAILGRSHVEKNLRDVRDIAMTFAGIDPADSEEQTKDNMQGMPANESEYGQAMAKQKGWIPIKPMQHYSMG
GVRTNPKGETHLKGLFCAGEAACWDLHGFNRLGGNSVSEAVVAGMIIGDYFASHCLEAQIEINTQKIEAFIKESQNYMHF
LLHNEGDEDVYEIRERMKEVMDEKVGVFREGKRLEEALKELQELYARSKNICVKNKVLHNNPELEDAYRTKKMLKLALCI
TQGALLRTESRGAHTRIDYPKRDDEKWLNRTLASWPNAEQDMPTIEYEELDVMKMEISPDFRGYGKKGNFIPHPKKEERD
AEILKTILELEKLGKDRVEVQHALMPFELQEKYKARNMRLEDEEVRARGEHLYSFNVHDLLDKHNANLKGEHHG

Sequences:

>Translated_714_residues
MKITYCDALIIGGGLAGLRASIACKQKGLNTIVLSLVPVRRSHSAAAQGGMQASLANAKKSEGDNEDLHFLDTVKGSDWG
CDQQVARMFVTTAPKAIRELASWGVPWTRIKKGDRSAVVNGEHVTITERDDRHGYILSRDFGGTKKWRTCFTADATGHTM
LYAVANEALHHKVDIQDRKDMLAFIHHDNKCYGAVVRDLITGEISAYVSKGTLLATGGYGRVYKHTTNAVICDGAGAASA
LETGVAKLGNMEAVQFHPTALVPSGILMTEGCRGDGGVLRDKFGRRFMPAYEPEKKELASRDVVSRRILEHIQKGYGAKS
PYGDHVWLDIAILGRSHVEKNLRDVRDIAMTFAGIDPADSEEQTKDNMQGMPANESEYGQAMAKQKGWIPIKPMQHYSMG
GVRTNPKGETHLKGLFCAGEAACWDLHGFNRLGGNSVSEAVVAGMIIGDYFASHCLEAQIEINTQKIEAFIKESQNYMHF
LLHNEGDEDVYEIRERMKEVMDEKVGVFREGKRLEEALKELQELYARSKNICVKNKVLHNNPELEDAYRTKKMLKLALCI
TQGALLRTESRGAHTRIDYPKRDDEKWLNRTLASWPNAEQDMPTIEYEELDVMKMEISPDFRGYGKKGNFIPHPKKEERD
AEILKTILELEKLGKDRVEVQHALMPFELQEKYKARNMRLEDEEVRARGEHLYSFNVHDLLDKHNANLKGEHHG
>Mature_714_residues
MKITYCDALIIGGGLAGLRASIACKQKGLNTIVLSLVPVRRSHSAAAQGGMQASLANAKKSEGDNEDLHFLDTVKGSDWG
CDQQVARMFVTTAPKAIRELASWGVPWTRIKKGDRSAVVNGEHVTITERDDRHGYILSRDFGGTKKWRTCFTADATGHTM
LYAVANEALHHKVDIQDRKDMLAFIHHDNKCYGAVVRDLITGEISAYVSKGTLLATGGYGRVYKHTTNAVICDGAGAASA
LETGVAKLGNMEAVQFHPTALVPSGILMTEGCRGDGGVLRDKFGRRFMPAYEPEKKELASRDVVSRRILEHIQKGYGAKS
PYGDHVWLDIAILGRSHVEKNLRDVRDIAMTFAGIDPADSEEQTKDNMQGMPANESEYGQAMAKQKGWIPIKPMQHYSMG
GVRTNPKGETHLKGLFCAGEAACWDLHGFNRLGGNSVSEAVVAGMIIGDYFASHCLEAQIEINTQKIEAFIKESQNYMHF
LLHNEGDEDVYEIRERMKEVMDEKVGVFREGKRLEEALKELQELYARSKNICVKNKVLHNNPELEDAYRTKKMLKLALCI
TQGALLRTESRGAHTRIDYPKRDDEKWLNRTLASWPNAEQDMPTIEYEELDVMKMEISPDFRGYGKKGNFIPHPKKEERD
AEILKTILELEKLGKDRVEVQHALMPFELQEKYKARNMRLEDEEVRARGEHLYSFNVHDLLDKHNANLKGEHHG

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=578, Percent_Identity=33.5640138408304, Blast_Score=279, Evalue=9e-75,
Organism=Escherichia coli, GI1786942, Length=597, Percent_Identity=35.1758793969849, Blast_Score=301, Evalue=1e-82,
Organism=Escherichia coli, GI1790597, Length=613, Percent_Identity=33.931484502447, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788928, Length=587, Percent_Identity=29.8126064735945, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=579, Percent_Identity=35.0604490500864, Blast_Score=294, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17550100, Length=573, Percent_Identity=35.0785340314136, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71986328, Length=479, Percent_Identity=24.0083507306889, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322701, Length=644, Percent_Identity=33.2298136645963, Blast_Score=291, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6322416, Length=618, Percent_Identity=32.2006472491909, Blast_Score=281, Evalue=3e-76,
Organism=Drosophila melanogaster, GI17137288, Length=610, Percent_Identity=33.7704918032787, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24655642, Length=610, Percent_Identity=33.7704918032787, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24655647, Length=610, Percent_Identity=33.7704918032787, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24663005, Length=595, Percent_Identity=32.7731092436975, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 80035; Mature: 80035

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00504 FRD_SDH_FAD_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITYCDALIIGGGLAGLRASIACKQKGLNTIVLSLVPVRRSHSAAAQGGMQASLANAKK
CEEEEEEHEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHC
SEGDNEDLHFLDTVKGSDWGCDQQVARMFVTTAPKAIRELASWGVPWTRIKKGDRSAVVN
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCCEEEC
GEHVTITERDDRHGYILSRDFGGTKKWRTCFTADATGHTMLYAVANEALHHKVDIQDRKD
CCEEEEEECCCCCCEEEECCCCCCCHHCEEEEECCCCCEEEHHHHHHHHHCCCCCCCHHH
MLAFIHHDNKCYGAVVRDLITGEISAYVSKGTLLATGGYGRVYKHTTNAVICDGAGAASA
HEEEHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCEEEECCCCHHHH
LETGVAKLGNMEAVQFHPTALVPSGILMTEGCRGDGGVLRDKFGRRFMPAYEPEKKELAS
HHHHHHHHCCCCEEEECCCEECCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHH
RDVVSRRILEHIQKGYGAKSPYGDHVWLDIAILGRSHVEKNLRDVRDIAMTFAGIDPADS
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
EEQTKDNMQGMPANESEYGQAMAKQKGWIPIKPMQHYSMGGVRTNPKGETHLKGLFCAGE
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCHHCEEEECCC
AACWDLHGFNRLGGNSVSEAVVAGMIIGDYFASHCLEAQIEINTQKIEAFIKESQNYMHF
CCEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCEEEE
LLHNEGDEDVYEIRERMKEVMDEKVGVFREGKRLEEALKELQELYARSKNICVKNKVLHN
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCEECC
NPELEDAYRTKKMLKLALCITQGALLRTESRGAHTRIDYPKRDDEKWLNRTLASWPNAEQ
CCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
DMPTIEYEELDVMKMEISPDFRGYGKKGNFIPHPKKEERDAEILKTILELEKLGKDRVEV
CCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
QHALMPFELQEKYKARNMRLEDEEVRARGEHLYSFNVHDLLDKHNANLKGEHHG
HHHHCCHHHHHHHHHHCCCCCHHHHHHCCCEEEECCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKITYCDALIIGGGLAGLRASIACKQKGLNTIVLSLVPVRRSHSAAAQGGMQASLANAKK
CEEEEEEHEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHC
SEGDNEDLHFLDTVKGSDWGCDQQVARMFVTTAPKAIRELASWGVPWTRIKKGDRSAVVN
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCCEEEC
GEHVTITERDDRHGYILSRDFGGTKKWRTCFTADATGHTMLYAVANEALHHKVDIQDRKD
CCEEEEEECCCCCCEEEECCCCCCCHHCEEEEECCCCCEEEHHHHHHHHHCCCCCCCHHH
MLAFIHHDNKCYGAVVRDLITGEISAYVSKGTLLATGGYGRVYKHTTNAVICDGAGAASA
HEEEHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCEEEECCCCHHHH
LETGVAKLGNMEAVQFHPTALVPSGILMTEGCRGDGGVLRDKFGRRFMPAYEPEKKELAS
HHHHHHHHCCCCEEEECCCEECCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHH
RDVVSRRILEHIQKGYGAKSPYGDHVWLDIAILGRSHVEKNLRDVRDIAMTFAGIDPADS
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
EEQTKDNMQGMPANESEYGQAMAKQKGWIPIKPMQHYSMGGVRTNPKGETHLKGLFCAGE
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCHHCEEEECCC
AACWDLHGFNRLGGNSVSEAVVAGMIIGDYFASHCLEAQIEINTQKIEAFIKESQNYMHF
CCEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCEEEE
LLHNEGDEDVYEIRERMKEVMDEKVGVFREGKRLEEALKELQELYARSKNICVKNKVLHN
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCEECC
NPELEDAYRTKKMLKLALCITQGALLRTESRGAHTRIDYPKRDDEKWLNRTLASWPNAEQ
CCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
DMPTIEYEELDVMKMEISPDFRGYGKKGNFIPHPKKEERDAEILKTILELEKLGKDRVEV
CCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
QHALMPFELQEKYKARNMRLEDEEVRARGEHLYSFNVHDLLDKHNANLKGEHHG
HHHHCCHHHHHHHHHHCCCCCHHHHHHCCCEEEECCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]