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The map label for this gene is pyrF [H]

Identifier: 109946875

GI number: 109946875

Start: 212815

End: 213498

Strand: Direct

Name: pyrF [H]

Synonym: Hac_0258

Alternate gene names: 109946875

Gene position: 212815-213498 (Clockwise)

Preceding gene: 109946869

Following gene: 109946876

Centisome position: 13.7

GC content: 38.16

Gene sequence:

>684_bases
ATGCAATTATGTGTCGCGCTAGACTTGGAAGAAAAAAAAGACAACCTTTCTTTATTGAAAGAATTGAAAGGCTTGAATTT
ATGGGCTAAAGTGGGGCTTAGATCGTTTATAAGAGATGGGGCTATTTTTTTAGATGAGATCAGAAAGATTGATGGAAATT
TTAAGATTTTTTTGGATTTGAAGCTTTATGATATTCCCTATACTATGGCAAATGCCGCGCTAGAATGCACGAAATTAGAA
ATTGATATGCTCACCGTGCATTTAAGTAGCGGTAAAATCGTGCTAACAACCTTAATGCAACGCCTAAACACTCTTAAAAA
ACGCCCCTTGATCATGGGCGTGAGCGCTTTAACGAGCTTTAGCGAAGAGGAATTTTTGAGCGTGTATAACGCCCCTTTAA
AAACTCAAGCGATCAAATTAAGCGTTATGGGTAAAGAGAGCGGTATTGATGGGGTGGTGTGTTCGGTGTTTGAAAGTTTA
GCGATTAAAGAAAATTTAGGTAAGGACTTTTTGACTTTAACCCCTGGTATAAGACTCAATAAAAACGATAAAGAAGATCA
AGAAAGGGTGGCTAACGCTAAAGAAGCTAAACAAAATTTAAGCGATTTTATCGTGGTGGGCCGCCCCATTTACCAAGCTA
AAGAGCCTAGAGAAGTGGCTTTAGAGCTTTTAAAGGATTGTTGA

Upstream 100 bases:

>100_bases
TTTCATGAAACTTTGTTTTATTTTCAAACGCTTATCATAGCTAAAATTCTTTCATTTCTTTTTGTTATAATGGCGTTATT
TTACACTTTAAAGGGCTTTC

Downstream 100 bases:

>100_bases
GAATGCAAGTTTTAGAAACGATTTCTGATTTAAGAGAATATCGTAAAAATGTAAAAGAAAGCGTTGGTTTTGTGCCGACT
ATGGGAGCCTTACACAAAGG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MQLCVALDLEEKKDNLSLLKELKGLNLWAKVGLRSFIRDGAIFLDEIRKIDGNFKIFLDLKLYDIPYTMANAALECTKLE
IDMLTVHLSSGKIVLTTLMQRLNTLKKRPLIMGVSALTSFSEEEFLSVYNAPLKTQAIKLSVMGKESGIDGVVCSVFESL
AIKENLGKDFLTLTPGIRLNKNDKEDQERVANAKEAKQNLSDFIVVGRPIYQAKEPREVALELLKDC

Sequences:

>Translated_227_residues
MQLCVALDLEEKKDNLSLLKELKGLNLWAKVGLRSFIRDGAIFLDEIRKIDGNFKIFLDLKLYDIPYTMANAALECTKLE
IDMLTVHLSSGKIVLTTLMQRLNTLKKRPLIMGVSALTSFSEEEFLSVYNAPLKTQAIKLSVMGKESGIDGVVCSVFESL
AIKENLGKDFLTLTPGIRLNKNDKEDQERVANAKEAKQNLSDFIVVGRPIYQAKEPREVALELLKDC
>Mature_227_residues
MQLCVALDLEEKKDNLSLLKELKGLNLWAKVGLRSFIRDGAIFLDEIRKIDGNFKIFLDLKLYDIPYTMANAALECTKLE
IDMLTVHLSSGKIVLTTLMQRLNTLKKRPLIMGVSALTSFSEEEFLSVYNAPLKTQAIKLSVMGKESGIDGVVCSVFESL
AIKENLGKDFLTLTPGIRLNKNDKEDQERVANAKEAKQNLSDFIVVGRPIYQAKEPREVALELLKDC

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=219, Percent_Identity=32.8767123287671, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25467; Mature: 25467

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLCVALDLEEKKDNLSLLKELKGLNLWAKVGLRSFIRDGAIFLDEIRKIDGNFKIFLDL
CCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEE
KLYDIPYTMANAALECTKLEIDMLTVHLSSGKIVLTTLMQRLNTLKKRPLIMGVSALTSF
EEEECCHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHCC
SEEEFLSVYNAPLKTQAIKLSVMGKESGIDGVVCSVFESLAIKENLGKDFLTLTPGIRLN
CHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEC
KNDKEDQERVANAKEAKQNLSDFIVVGRPIYQAKEPREVALELLKDC
CCCCHHHHHHHHHHHHHHHHHHEEEECCCHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQLCVALDLEEKKDNLSLLKELKGLNLWAKVGLRSFIRDGAIFLDEIRKIDGNFKIFLDL
CCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEE
KLYDIPYTMANAALECTKLEIDMLTVHLSSGKIVLTTLMQRLNTLKKRPLIMGVSALTSF
EEEECCHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHCC
SEEEFLSVYNAPLKTQAIKLSVMGKESGIDGVVCSVFESLAIKENLGKDFLTLTPGIRLN
CHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEC
KNDKEDQERVANAKEAKQNLSDFIVVGRPIYQAKEPREVALELLKDC
CCCCHHHHHHHHHHHHHHHHHHEEEECCCHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA