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The map label for this gene is cobT [H]

Identifier: 109946846

GI number: 109946846

Start: 194780

End: 195469

Strand: Reverse

Name: cobT [H]

Synonym: Hac_0226

Alternate gene names: 109946846

Gene position: 195469-194780 (Counterclockwise)

Preceding gene: 109946847

Following gene: 109946845

Centisome position: 12.58

GC content: 41.01

Gene sequence:

>690_bases
GTGAAAAATTTAGTGATCTTAAGTGGGGCTGGCATTTCAGCAGAAAGTGGGATTAAAACCTTTAGAGACGCCAATGGCTT
GTGGGAAGGGCATGACATCATGGAAGTCGCTTCGCCTTATGGCTGGAAAAAGAACCCACAAAAAGTGTTGGATTTTTACA
ATCAAAGGTGCAGACAGCTTTTTGAAGTCTATCCTAACAAAGCCCACAAGACTCTAGCGGAATTAGAAAAACACTATCAA
GTCAATATTATCACCCAAAATGTAGATGATTTGCATGAAAGGGCGGGCTCTTCTTGCATTTTACACTTGCATGGGGAATT
GTTGAGCGTTCGCAGTGAAAAAGATCCTCATTTGGTTTATAGGTGGGAAAAGGACTTGAATTTAGGCGATTTAGCCCAAG
ATAAAGTGCAATTACGCCCTGATATTGTGTGGTTTGGCGAAGCTGTCCCCTTGCTTAAAGAAGCGATTTCTTTAGTCAAA
CAAGCGCATGTTTTAATCATCATTGGCACTTCTTTGCAAGTCTATCCCGCAGCCAGTCTCTACACGCATGCCCATAAAGA
CGCTCTTATTTATTACATTGACCCTAAAGCTAAAAACGCCCATTTACCCCAAAATATCCAATGCATTAATGAAAGTGCAG
TGAATGCCATGCATAATTTAATGCCTAAACTCATAGAGATGGCCTCTTAA

Upstream 100 bases:

>100_bases
GCATGGGGTTACTCGTGGGGTTTGTTGCACCTTTTATTTTTAAGTTGTTTTTGCATGACAAATTCAGCGGCACTCATATT
GAAACCATCAAGGAGGAATA

Downstream 100 bases:

>100_bases
GAGGTTTTTAAATCTTTTTGAGATCCGTTATCATTGAATGATAAAATTTAAAAATGCTTAAAGGCTTTTTTAAAACTAAT
ATAATAAATAGCTTGATTTT

Product: nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphor

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKNLVILSGAGISAESGIKTFRDANGLWEGHDIMEVASPYGWKKNPQKVLDFYNQRCRQLFEVYPNKAHKTLAELEKHYQ
VNIITQNVDDLHERAGSSCILHLHGELLSVRSEKDPHLVYRWEKDLNLGDLAQDKVQLRPDIVWFGEAVPLLKEAISLVK
QAHVLIIIGTSLQVYPAASLYTHAHKDALIYYIDPKAKNAHLPQNIQCINESAVNAMHNLMPKLIEMAS

Sequences:

>Translated_229_residues
MKNLVILSGAGISAESGIKTFRDANGLWEGHDIMEVASPYGWKKNPQKVLDFYNQRCRQLFEVYPNKAHKTLAELEKHYQ
VNIITQNVDDLHERAGSSCILHLHGELLSVRSEKDPHLVYRWEKDLNLGDLAQDKVQLRPDIVWFGEAVPLLKEAISLVK
QAHVLIIIGTSLQVYPAASLYTHAHKDALIYYIDPKAKNAHLPQNIQCINESAVNAMHNLMPKLIEMAS
>Mature_229_residues
MKNLVILSGAGISAESGIKTFRDANGLWEGHDIMEVASPYGWKKNPQKVLDFYNQRCRQLFEVYPNKAHKTLAELEKHYQ
VNIITQNVDDLHERAGSSCILHLHGELLSVRSEKDPHLVYRWEKDLNLGDLAQDKVQLRPDIVWFGEAVPLLKEAISLVK
QAHVLIIIGTSLQVYPAASLYTHAHKDALIYYIDPKAKNAHLPQNIQCINESAVNAMHNLMPKLIEMAS

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=196, Percent_Identity=35.7142857142857, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI6912664, Length=208, Percent_Identity=33.6538461538462, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI13787215, Length=208, Percent_Identity=33.6538461538462, Blast_Score=114, Evalue=6e-26,
Organism=Escherichia coli, GI308199517, Length=189, Percent_Identity=44.973544973545, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 25909; Mature: 25909

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLVILSGAGISAESGIKTFRDANGLWEGHDIMEVASPYGWKKNPQKVLDFYNQRCRQL
CCCEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FEVYPNKAHKTLAELEKHYQVNIITQNVDDLHERAGSSCILHLHGELLSVRSEKDPHLVY
HHHCCCHHHHHHHHHHHCEEEEEEECCHHHHHHHCCCEEEEEECCHHHHHCCCCCCEEEE
RWEKDLNLGDLAQDKVQLRPDIVWFGEAVPLLKEAISLVKQAHVLIIIGTSLQVYPAASL
EECCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCEEEEECCCEEEEECHHH
YTHAHKDALIYYIDPKAKNAHLPQNIQCINESAVNAMHNLMPKLIEMAS
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNLVILSGAGISAESGIKTFRDANGLWEGHDIMEVASPYGWKKNPQKVLDFYNQRCRQL
CCCEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FEVYPNKAHKTLAELEKHYQVNIITQNVDDLHERAGSSCILHLHGELLSVRSEKDPHLVY
HHHCCCHHHHHHHHHHHCEEEEEEECCHHHHHHHCCCEEEEEECCHHHHHCCCCCCEEEE
RWEKDLNLGDLAQDKVQLRPDIVWFGEAVPLLKEAISLVKQAHVLIIIGTSLQVYPAASL
EECCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCEEEEECCCEEEEECHHH
YTHAHKDALIYYIDPKAKNAHLPQNIQCINESAVNAMHNLMPKLIEMAS
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]