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The map label for this gene is trmE [H]

Identifier: 109946790

GI number: 109946790

Start: 142790

End: 144142

Strand: Reverse

Name: trmE [H]

Synonym: Hac_0167

Alternate gene names: 109946790

Gene position: 144142-142790 (Counterclockwise)

Preceding gene: 109946791

Following gene: 109946757

Centisome position: 9.28

GC content: 37.84

Gene sequence:

>1353_bases
ATGAATGACACCATTGCCGCTGTCGCTACCCCATTGGGTAAAGGAGCGATTAGTATTGTTAAAATCAGCGGCAATAACGC
TTTAAACATTCTCAAACAACTCACCAAAAAACAAGACTTCACCCCTAGATACGCTTATGTGTGCGATATTTTTTCTAATA
ATATTTTATTGGACAAGGCGTTAGCGATTTATTTCAAAGCCCCTTATAGTTTCACCGGTGAAGATGTGTGCGAAATCCAA
TGCCACGGAAGCCCCCTTTTAACTCAAAACATCCTTCAAGCTTGTTTGAATTTAGGAGCTAGGCTTGCTCAAGCGGGGGA
ATTCAGTAAAAAAGCCTTTTTAAACCATAAAATGGATTTGAGCGAGATTGAAGCGAGTATCCGACTTATCCTATGTGAAG
ATGAAAGCGTTTTAAACGCCCTAGCCAGGCAACTTAAGGGTGAATTAAAAATCTTTATTGAAGAAGCTAGAAATAATCTT
TTGAAACTTCTAGCGAGCTCAGAAGTTTTGATTGATTATAGCGAAGAAGACATTCCTAGCGATTTTTTAAACGAAGTGTC
TCTAAGCCTAGAAAAACAAATCGCCTCTTTTAAGGACTTATTGGATTTTTCTAACATGCAAAAACAAAAAAATAAGGGGC
ATGCTTTAAGCATTATTGGCAAACCTAATGCGGGCAAAAGCTCCTTACTAAACGCCATGCTTTTAGAAGAAAGGGCTTTG
GTGAGCGACATTAAAGGCACAACAAGGGACACCATAGAAGAAGTCATTGAATTGCAAGGGCATAAGGTACGCTTGATTGA
CACTGCCGGCATTAGAGAGAGCGTGGATAAAATAGAGCGATTGGGGATTGAAAAAAGCCTTAAGAGTTTAGAAAATTGCG
ACATTATTTTGAGCGTGTTTGATCTTTCTAAACCCCTAGAAAAAGAAGATTTTAATATCATTGACGCTCTTAATCGCACT
AAAAAGCCTTGTATTGTTGTTTTGAATAAAAATGATTTATCGCCCAAGCTAGAGCTTGAAGTTTTAAAATCCCATCTTCA
AATCCCTTACTCCATGCTAGAGACAAACACCCTTAATTCCAAAGCTTGCTTGAAAGACTTAAGCCAAAAAATCAGCGCTT
TTTTCCCCAAACTAGACACTCAAAACAAGCTTTTACTCACTTCCCTAGCCCAAAAAAACGCCCTAGAAAACGCCATTACT
GAATTACAAAACGCTAAAAACCATTTAGAAACTTTAGAGCTTTTTTCTTACCACATTTTAAGCGCAATAGAAAACTTGAA
CTTGCTCACTCGCCCTTATGAAACTAGCCAAATGCTTGATAGCATGTTCAGTGAATTTTGTTTAGGAAAATGA

Upstream 100 bases:

>100_bases
ATCGCTTTAAAAAAATTAAGGAAAGCGTTCCCCAACAAATATGTCTCCATCAAAACCAACTTAAACGATGAAAAATGCAT
CGTCATTAACGACTTCCACC

Downstream 100 bases:

>100_bases
AACGCCTAAAAAAGGGGTTTTAGCATTATTTAAAAATTCATTATTAATGTTTAATGAGAAAAAACCAAAATTTTTTAGAA
TAGTTGTTTTAAAAAGCAAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNDTIAAVATPLGKGAISIVKISGNNALNILKQLTKKQDFTPRYAYVCDIFSNNILLDKALAIYFKAPYSFTGEDVCEIQ
CHGSPLLTQNILQACLNLGARLAQAGEFSKKAFLNHKMDLSEIEASIRLILCEDESVLNALARQLKGELKIFIEEARNNL
LKLLASSEVLIDYSEEDIPSDFLNEVSLSLEKQIASFKDLLDFSNMQKQKNKGHALSIIGKPNAGKSSLLNAMLLEERAL
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESVDKIERLGIEKSLKSLENCDIILSVFDLSKPLEKEDFNIIDALNRT
KKPCIVVLNKNDLSPKLELEVLKSHLQIPYSMLETNTLNSKACLKDLSQKISAFFPKLDTQNKLLLTSLAQKNALENAIT
ELQNAKNHLETLELFSYHILSAIENLNLLTRPYETSQMLDSMFSEFCLGK

Sequences:

>Translated_450_residues
MNDTIAAVATPLGKGAISIVKISGNNALNILKQLTKKQDFTPRYAYVCDIFSNNILLDKALAIYFKAPYSFTGEDVCEIQ
CHGSPLLTQNILQACLNLGARLAQAGEFSKKAFLNHKMDLSEIEASIRLILCEDESVLNALARQLKGELKIFIEEARNNL
LKLLASSEVLIDYSEEDIPSDFLNEVSLSLEKQIASFKDLLDFSNMQKQKNKGHALSIIGKPNAGKSSLLNAMLLEERAL
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESVDKIERLGIEKSLKSLENCDIILSVFDLSKPLEKEDFNIIDALNRT
KKPCIVVLNKNDLSPKLELEVLKSHLQIPYSMLETNTLNSKACLKDLSQKISAFFPKLDTQNKLLLTSLAQKNALENAIT
ELQNAKNHLETLELFSYHILSAIENLNLLTRPYETSQMLDSMFSEFCLGK
>Mature_450_residues
MNDTIAAVATPLGKGAISIVKISGNNALNILKQLTKKQDFTPRYAYVCDIFSNNILLDKALAIYFKAPYSFTGEDVCEIQ
CHGSPLLTQNILQACLNLGARLAQAGEFSKKAFLNHKMDLSEIEASIRLILCEDESVLNALARQLKGELKIFIEEARNNL
LKLLASSEVLIDYSEEDIPSDFLNEVSLSLEKQIASFKDLLDFSNMQKQKNKGHALSIIGKPNAGKSSLLNAMLLEERAL
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESVDKIERLGIEKSLKSLENCDIILSVFDLSKPLEKEDFNIIDALNRT
KKPCIVVLNKNDLSPKLELEVLKSHLQIPYSMLETNTLNSKACLKDLSQKISAFFPKLDTQNKLLLTSLAQKNALENAIT
ELQNAKNHLETLELFSYHILSAIENLNLLTRPYETSQMLDSMFSEFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=31.8965517241379, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI193082993, Length=455, Percent_Identity=31.6483516483516, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=30.0403225806452, Blast_Score=183, Evalue=4e-46,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=33.982683982684, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI87082120, Length=120, Percent_Identity=35.8333333333333, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=29.4117647058824, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=32.3828920570265, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI45550813, Length=304, Percent_Identity=33.2236842105263, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50235; Mature: 50235

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTIAAVATPLGKGAISIVKISGNNALNILKQLTKKQDFTPRYAYVCDIFSNNILLDKA
CCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHEEHHHCCCEEHHHH
LAIYFKAPYSFTGEDVCEIQCHGSPLLTQNILQACLNLGARLAQAGEFSKKAFLNHKMDL
HHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
SEIEASIRLILCEDESVLNALARQLKGELKIFIEEARNNLLKLLASSEVLIDYSEEDIPS
HHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCH
DFLNEVSLSLEKQIASFKDLLDFSNMQKQKNKGHALSIIGKPNAGKSSLLNAMLLEERAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESVDKIERLGIEKSLKSLENCDIILSVF
HHHHCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEHH
DLSKPLEKEDFNIIDALNRTKKPCIVVLNKNDLSPKLELEVLKSHLQIPYSMLETNTLNS
HCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCH
KACLKDLSQKISAFFPKLDTQNKLLLTSLAQKNALENAITELQNAKNHLETLELFSYHIL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIENLNLLTRPYETSQMLDSMFSEFCLGK
HHHHHCHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDTIAAVATPLGKGAISIVKISGNNALNILKQLTKKQDFTPRYAYVCDIFSNNILLDKA
CCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHEEHHHCCCEEHHHH
LAIYFKAPYSFTGEDVCEIQCHGSPLLTQNILQACLNLGARLAQAGEFSKKAFLNHKMDL
HHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
SEIEASIRLILCEDESVLNALARQLKGELKIFIEEARNNLLKLLASSEVLIDYSEEDIPS
HHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCH
DFLNEVSLSLEKQIASFKDLLDFSNMQKQKNKGHALSIIGKPNAGKSSLLNAMLLEERAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESVDKIERLGIEKSLKSLENCDIILSVF
HHHHCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEHH
DLSKPLEKEDFNIIDALNRTKKPCIVVLNKNDLSPKLELEVLKSHLQIPYSMLETNTLNS
HCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCH
KACLKDLSQKISAFFPKLDTQNKLLLTSLAQKNALENAITELQNAKNHLETLELFSYHIL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIENLNLLTRPYETSQMLDSMFSEFCLGK
HHHHHCHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA