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The map label for this gene is miaA

Identifier: 109946661

GI number: 109946661

Start: 25891

End: 26691

Strand: Reverse

Name: miaA

Synonym: Hac_0022

Alternate gene names: 109946661

Gene position: 26691-25891 (Counterclockwise)

Preceding gene: 109946662

Following gene: 109946660

Centisome position: 1.72

GC content: 37.2

Gene sequence:

>801_bases
TTGAGTATTTATAAAGATATTAATATCGCTTCGGCTAAACCCAGCCTAAAAGAGCGAAAAAATATCAAACATTACGCCCT
AGACTGCCTTAACATTGATGAAAAAAATAACGCCCCCCTTTTTAAAACCCTTTTAGAAGACGCCATGAAAGTATCCCAAA
AAGAGATTTTACTCATTGTGGGGGGGAGCAGTTTTTACCTCAAATCCATTTTAGAAGGTTTGAGCAGCATGCCAAAACTT
AATAACGATCAAATTTTAAAAATAGAGCGAGAAATTGCCACCCTGACTAACCCTTACGCCTTTTTAAAATCCATTGACCC
TAACATGGCTTTTAAAATTCATTCAAACGACACTTACCGCATTCATAAGGCTTTAGAAATCTTTTATGCCACCCATACGC
CCCCAAGCGAGTATTTTAAAAAAAATCCCAAAAAGCCCTTTGAGCATGCCATAACGCTATTCGCTCTTTGTATTGAAAAA
ACCGTACTCCATAACCGCATCAAACAGCGCACTAAAAACATGCTTGATTGCGGTCTTATTGAAGAAATCAAAGCCCTTTA
TACCCAATACCCTAAAGATTCACAGCCTTTTAAAGCCATAGGCGTTAAAGAGAGCATTCTTTATTTAGAAAAACAGCTCA
CTTTAAAAGAATTAGAAGAAGCGATTGTCTCTAACACCATAAAATTAGCCAAGCGGCAAAACACTTTCAATAAAACCCAA
TTCAACAACCTTTATGTGGGGAGCGTTGAAGAAGTTAGGCATGCGATTTTAAAGCGCTCAAAAAGCGGTATTAAAGAATA
G

Upstream 100 bases:

>100_bases
GAAACTTATCGCGCTTCTAGGGCCTAGCGGGAGCGGGAAAAGCGCTCTTTCTATTGAACTAGCCCAAACATTAGACGCAG
AAATCTTTTCTTTGGACTCT

Downstream 100 bases:

>100_bases
CCCATGAACACGCAAATCTCACCCAATCAAGTTATCCCTATTTTTATGAGTTTTGATAAAAATTACGTGCTAGGGGCAGG
TGTGAGCCTTTATTCTTTAC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSIYKDINIASAKPSLKERKNIKHYALDCLNIDEKNNAPLFKTLLEDAMKVSQKEILLIVGGSSFYLKSILEGLSSMPKL
NNDQILKIEREIATLTNPYAFLKSIDPNMAFKIHSNDTYRIHKALEIFYATHTPPSEYFKKNPKKPFEHAITLFALCIEK
TVLHNRIKQRTKNMLDCGLIEEIKALYTQYPKDSQPFKAIGVKESILYLEKQLTLKELEEAIVSNTIKLAKRQNTFNKTQ
FNNLYVGSVEEVRHAILKRSKSGIKE

Sequences:

>Translated_266_residues
MSIYKDINIASAKPSLKERKNIKHYALDCLNIDEKNNAPLFKTLLEDAMKVSQKEILLIVGGSSFYLKSILEGLSSMPKL
NNDQILKIEREIATLTNPYAFLKSIDPNMAFKIHSNDTYRIHKALEIFYATHTPPSEYFKKNPKKPFEHAITLFALCIEK
TVLHNRIKQRTKNMLDCGLIEEIKALYTQYPKDSQPFKAIGVKESILYLEKQLTLKELEEAIVSNTIKLAKRQNTFNKTQ
FNNLYVGSVEEVRHAILKRSKSGIKE
>Mature_265_residues
SIYKDINIASAKPSLKERKNIKHYALDCLNIDEKNNAPLFKTLLEDAMKVSQKEILLIVGGSSFYLKSILEGLSSMPKLN
NDQILKIEREIATLTNPYAFLKSIDPNMAFKIHSNDTYRIHKALEIFYATHTPPSEYFKKNPKKPFEHAITLFALCIEKT
VLHNRIKQRTKNMLDCGLIEEIKALYTQYPKDSQPFKAIGVKESILYLEKQLTLKELEEAIVSNTIKLAKRQNTFNKTQF
NNLYVGSVEEVRHAILKRSKSGIKE

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=283, Percent_Identity=25.4416961130742, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1790613, Length=265, Percent_Identity=29.4339622641509, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25144712, Length=238, Percent_Identity=25.2100840336134, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324848, Length=256, Percent_Identity=30.859375, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24649830, Length=267, Percent_Identity=25.4681647940075, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_HELAH (Q17ZN6)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_663889.1
- ProteinModelPortal:   Q17ZN6
- SMR:   Q17ZN6
- STRING:   Q17ZN6
- GeneID:   4176766
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0022
- NMPDR:   fig|382638.8.peg.22
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   KEVRHAI
- ProtClustDB:   PRK00091
- BioCyc:   HACI382638:HAC_0022-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 30580; Mature: 30449

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYKDINIASAKPSLKERKNIKHYALDCLNIDEKNNAPLFKTLLEDAMKVSQKEILLIV
CCCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
GGSSFYLKSILEGLSSMPKLNNDQILKIEREIATLTNPYAFLKSIDPNMAFKIHSNDTYR
CCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHH
IHKALEIFYATHTPPSEYFKKNPKKPFEHAITLFALCIEKTVLHNRIKQRTKNMLDCGLI
HHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EEIKALYTQYPKDSQPFKAIGVKESILYLEKQLTLKELEEAIVSNTIKLAKRQNTFNKTQ
HHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNNLYVGSVEEVRHAILKRSKSGIKE
CCCEEECCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIYKDINIASAKPSLKERKNIKHYALDCLNIDEKNNAPLFKTLLEDAMKVSQKEILLIV
CCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
GGSSFYLKSILEGLSSMPKLNNDQILKIEREIATLTNPYAFLKSIDPNMAFKIHSNDTYR
CCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHH
IHKALEIFYATHTPPSEYFKKNPKKPFEHAITLFALCIEKTVLHNRIKQRTKNMLDCGLI
HHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EEIKALYTQYPKDSQPFKAIGVKESILYLEKQLTLKELEEAIVSNTIKLAKRQNTFNKTQ
HHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNNLYVGSVEEVRHAILKRSKSGIKE
CCCEEECCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA